Metal exposure from additive manufacturing and its effect on the nasal lavage fluid proteome - a pilot study.

The use of metal additive manufacturing (AM) is steadily increasing and is an emerging concern regarding occupational exposure. In this study, non-invasive sampled nasal lavage fluid (NLF) from the upper airways was collected from metal AM operators at the beginning and end of a workweek during two...

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Autores principales:	Maria Assenhöj, Liam J Ward, Bijar Ghafouri, Pål Graff, Stefan A Ljunggren
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f2fce3edbff6422eab2eb3c5fe8719c6
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spelling	oai:doaj.org-article:f2fce3edbff6422eab2eb3c5fe8719c62021-12-02T20:14:51ZMetal exposure from additive manufacturing and its effect on the nasal lavage fluid proteome - a pilot study.1932-620310.1371/journal.pone.0256746https://doaj.org/article/f2fce3edbff6422eab2eb3c5fe8719c62021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256746https://doaj.org/toc/1932-6203The use of metal additive manufacturing (AM) is steadily increasing and is an emerging concern regarding occupational exposure. In this study, non-invasive sampled nasal lavage fluid (NLF) from the upper airways was collected from metal AM operators at the beginning and end of a workweek during two consecutive years with preventive interventions in the occupational setting in-between (n = 5 year 1, n = 9 year 2). During year one, NLF was also collected from welders (n = 6) from the same company to get a comparison with a traditional manufacturing technique with known exposure and health risks. The samples were investigated using untargeted proteomics, as well as using multi-immunoassay to analyze a panel of 71 inflammatory protein markers. NLF in AM operators from year 1 showed decreased levels of Immunoglobulin J and WAP four-disulfide core domain protein 2 and increased levels of Golgi membrane protein 1, Uteroglobin and Protein S100-A6 at the end of the workweek. At year two, after preventive interventions, there were no significant differences at the end of the workweek. In welders, Annexin A1 and Protein S100-A6 were increased at the end of the workweek. The analysis of 71 inflammatory biomarkers showed no significant differences between the beginning and the end of workweek year 1 in AM operators. We identified several proteins of interest in the AM operators that could serve as possible markers for exposure in future studies with a larger cohort for validation.Maria AssenhöjLiam J WardBijar GhafouriPål GraffStefan A LjunggrenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0256746 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria Assenhöj Liam J Ward Bijar Ghafouri Pål Graff Stefan A Ljunggren Metal exposure from additive manufacturing and its effect on the nasal lavage fluid proteome - a pilot study.
description	The use of metal additive manufacturing (AM) is steadily increasing and is an emerging concern regarding occupational exposure. In this study, non-invasive sampled nasal lavage fluid (NLF) from the upper airways was collected from metal AM operators at the beginning and end of a workweek during two consecutive years with preventive interventions in the occupational setting in-between (n = 5 year 1, n = 9 year 2). During year one, NLF was also collected from welders (n = 6) from the same company to get a comparison with a traditional manufacturing technique with known exposure and health risks. The samples were investigated using untargeted proteomics, as well as using multi-immunoassay to analyze a panel of 71 inflammatory protein markers. NLF in AM operators from year 1 showed decreased levels of Immunoglobulin J and WAP four-disulfide core domain protein 2 and increased levels of Golgi membrane protein 1, Uteroglobin and Protein S100-A6 at the end of the workweek. At year two, after preventive interventions, there were no significant differences at the end of the workweek. In welders, Annexin A1 and Protein S100-A6 were increased at the end of the workweek. The analysis of 71 inflammatory biomarkers showed no significant differences between the beginning and the end of workweek year 1 in AM operators. We identified several proteins of interest in the AM operators that could serve as possible markers for exposure in future studies with a larger cohort for validation.
format	article
author	Maria Assenhöj Liam J Ward Bijar Ghafouri Pål Graff Stefan A Ljunggren
author_facet	Maria Assenhöj Liam J Ward Bijar Ghafouri Pål Graff Stefan A Ljunggren
author_sort	Maria Assenhöj
title	Metal exposure from additive manufacturing and its effect on the nasal lavage fluid proteome - a pilot study.
title_short	Metal exposure from additive manufacturing and its effect on the nasal lavage fluid proteome - a pilot study.
title_full	Metal exposure from additive manufacturing and its effect on the nasal lavage fluid proteome - a pilot study.
title_fullStr	Metal exposure from additive manufacturing and its effect on the nasal lavage fluid proteome - a pilot study.
title_full_unstemmed	Metal exposure from additive manufacturing and its effect on the nasal lavage fluid proteome - a pilot study.
title_sort	metal exposure from additive manufacturing and its effect on the nasal lavage fluid proteome - a pilot study.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f2fce3edbff6422eab2eb3c5fe8719c6
work_keys_str_mv	AT mariaassenhoj metalexposurefromadditivemanufacturinganditseffectonthenasallavagefluidproteomeapilotstudy AT liamjward metalexposurefromadditivemanufacturinganditseffectonthenasallavagefluidproteomeapilotstudy AT bijarghafouri metalexposurefromadditivemanufacturinganditseffectonthenasallavagefluidproteomeapilotstudy AT palgraff metalexposurefromadditivemanufacturinganditseffectonthenasallavagefluidproteomeapilotstudy AT stefanaljunggren metalexposurefromadditivemanufacturinganditseffectonthenasallavagefluidproteomeapilotstudy
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Metal exposure from additive manufacturing and its effect on the nasal lavage fluid proteome - a pilot study.

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