Relationship between dental and periodontal health status and the salivary microbiome: bacterial diversity, co-occurrence networks and predictive models

Abstract The present study used 16S rRNA gene amplicon sequencing to assess the impact on salivary microbiome of different grades of dental and periodontal disease and the combination of both (hereinafter referred to as oral disease), in terms of bacterial diversity, co-occurrence network patterns a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	M. Relvas, A. Regueira-Iglesias, C. Balsa-Castro, F. Salazar, J. J. Pacheco, C. Cabral, C. Henriques, I. Tomás
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f2fd54817ae44fa180d44b5518cf5d8d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f2fd54817ae44fa180d44b5518cf5d8d
record_format	dspace
spelling	oai:doaj.org-article:f2fd54817ae44fa180d44b5518cf5d8d2021-12-02T14:01:22ZRelationship between dental and periodontal health status and the salivary microbiome: bacterial diversity, co-occurrence networks and predictive models10.1038/s41598-020-79875-x2045-2322https://doaj.org/article/f2fd54817ae44fa180d44b5518cf5d8d2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79875-xhttps://doaj.org/toc/2045-2322Abstract The present study used 16S rRNA gene amplicon sequencing to assess the impact on salivary microbiome of different grades of dental and periodontal disease and the combination of both (hereinafter referred to as oral disease), in terms of bacterial diversity, co-occurrence network patterns and predictive models. Our scale of overall oral health was used to produce a convenience sample of 81 patients from 270 who were initially recruited. Saliva samples were collected from each participant. Sequencing was performed in Illumina MiSeq with 2 × 300 bp reads, while the raw reads were processed according to the Mothur pipeline. The statistical analysis of the 16S rDNA sequencing data at the species level was conducted using the phyloseq, DESeq2, Microbiome, SpiecEasi, igraph, MixOmics packages. The simultaneous presence of dental and periodontal pathology has a potentiating effect on the richness and diversity of the salivary microbiota. The structure of the bacterial community in oral health differs from that present in dental, periodontal or oral disease, especially in high grades. Supragingival dental parameters influence the microbiota’s abundance more than subgingival periodontal parameters, with the former making a greater contribution to the impact that oral health has on the salivary microbiome. The possible keystone OTUs are different in the oral health and disease, and even these vary between dental and periodontal disease: half of them belongs to the core microbiome and are independent of the abundance parameters. The salivary microbiome, involving a considerable number of OTUs, shows an excellent discriminatory potential for distinguishing different grades of dental, periodontal or oral disease; considering the number of predictive OTUs, the best model is that which predicts the combined dental and periodontal status.M. RelvasA. Regueira-IglesiasC. Balsa-CastroF. SalazarJ. J. PachecoC. CabralC. HenriquesI. TomásNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-22 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. Relvas A. Regueira-Iglesias C. Balsa-Castro F. Salazar J. J. Pacheco C. Cabral C. Henriques I. Tomás Relationship between dental and periodontal health status and the salivary microbiome: bacterial diversity, co-occurrence networks and predictive models
description	Abstract The present study used 16S rRNA gene amplicon sequencing to assess the impact on salivary microbiome of different grades of dental and periodontal disease and the combination of both (hereinafter referred to as oral disease), in terms of bacterial diversity, co-occurrence network patterns and predictive models. Our scale of overall oral health was used to produce a convenience sample of 81 patients from 270 who were initially recruited. Saliva samples were collected from each participant. Sequencing was performed in Illumina MiSeq with 2 × 300 bp reads, while the raw reads were processed according to the Mothur pipeline. The statistical analysis of the 16S rDNA sequencing data at the species level was conducted using the phyloseq, DESeq2, Microbiome, SpiecEasi, igraph, MixOmics packages. The simultaneous presence of dental and periodontal pathology has a potentiating effect on the richness and diversity of the salivary microbiota. The structure of the bacterial community in oral health differs from that present in dental, periodontal or oral disease, especially in high grades. Supragingival dental parameters influence the microbiota’s abundance more than subgingival periodontal parameters, with the former making a greater contribution to the impact that oral health has on the salivary microbiome. The possible keystone OTUs are different in the oral health and disease, and even these vary between dental and periodontal disease: half of them belongs to the core microbiome and are independent of the abundance parameters. The salivary microbiome, involving a considerable number of OTUs, shows an excellent discriminatory potential for distinguishing different grades of dental, periodontal or oral disease; considering the number of predictive OTUs, the best model is that which predicts the combined dental and periodontal status.
format	article
author	M. Relvas A. Regueira-Iglesias C. Balsa-Castro F. Salazar J. J. Pacheco C. Cabral C. Henriques I. Tomás
author_facet	M. Relvas A. Regueira-Iglesias C. Balsa-Castro F. Salazar J. J. Pacheco C. Cabral C. Henriques I. Tomás
author_sort	M. Relvas
title	Relationship between dental and periodontal health status and the salivary microbiome: bacterial diversity, co-occurrence networks and predictive models
title_short	Relationship between dental and periodontal health status and the salivary microbiome: bacterial diversity, co-occurrence networks and predictive models
title_full	Relationship between dental and periodontal health status and the salivary microbiome: bacterial diversity, co-occurrence networks and predictive models
title_fullStr	Relationship between dental and periodontal health status and the salivary microbiome: bacterial diversity, co-occurrence networks and predictive models
title_full_unstemmed	Relationship between dental and periodontal health status and the salivary microbiome: bacterial diversity, co-occurrence networks and predictive models
title_sort	relationship between dental and periodontal health status and the salivary microbiome: bacterial diversity, co-occurrence networks and predictive models
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f2fd54817ae44fa180d44b5518cf5d8d
work_keys_str_mv	AT mrelvas relationshipbetweendentalandperiodontalhealthstatusandthesalivarymicrobiomebacterialdiversitycooccurrencenetworksandpredictivemodels AT aregueiraiglesias relationshipbetweendentalandperiodontalhealthstatusandthesalivarymicrobiomebacterialdiversitycooccurrencenetworksandpredictivemodels AT cbalsacastro relationshipbetweendentalandperiodontalhealthstatusandthesalivarymicrobiomebacterialdiversitycooccurrencenetworksandpredictivemodels AT fsalazar relationshipbetweendentalandperiodontalhealthstatusandthesalivarymicrobiomebacterialdiversitycooccurrencenetworksandpredictivemodels AT jjpacheco relationshipbetweendentalandperiodontalhealthstatusandthesalivarymicrobiomebacterialdiversitycooccurrencenetworksandpredictivemodels AT ccabral relationshipbetweendentalandperiodontalhealthstatusandthesalivarymicrobiomebacterialdiversitycooccurrencenetworksandpredictivemodels AT chenriques relationshipbetweendentalandperiodontalhealthstatusandthesalivarymicrobiomebacterialdiversitycooccurrencenetworksandpredictivemodels AT itomas relationshipbetweendentalandperiodontalhealthstatusandthesalivarymicrobiomebacterialdiversitycooccurrencenetworksandpredictivemodels
_version_	1718392177917165568

Relationship between dental and periodontal health status and the salivary microbiome: bacterial diversity, co-occurrence networks and predictive models

Ejemplares similares