Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States.

Salmonella enterica subspecies enterica serotype Dublin is a host-adapted serotype in cattle, associated with enteritis and systemic disease. The primary clinical manifestation of Salmonella Dublin infection in cattle, especially calves, is respiratory disease. While rare in humans, it can cause sev...

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Autores principales:	Mariela E Srednik, Kristina Lantz, Jessica A Hicks, Brenda R Morningstar-Shaw, Tonya A Mackie, Linda K Schlater
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f2fdeda4b687467f8a963b94c19861d2
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spelling	oai:doaj.org-article:f2fdeda4b687467f8a963b94c19861d22021-12-02T20:08:08ZAntimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States.1932-620310.1371/journal.pone.0249617https://doaj.org/article/f2fdeda4b687467f8a963b94c19861d22021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0249617https://doaj.org/toc/1932-6203Salmonella enterica subspecies enterica serotype Dublin is a host-adapted serotype in cattle, associated with enteritis and systemic disease. The primary clinical manifestation of Salmonella Dublin infection in cattle, especially calves, is respiratory disease. While rare in humans, it can cause severe illness, including bacteremia, with hospitalization and death. In the United States, S. Dublin has become one of the most multidrug-resistant serotypes. The objective of this study was to characterize S. Dublin isolates from sick cattle by analyzing phenotypic and genotypic antimicrobial resistance (AMR) profiles, the presence of plasmids, and phylogenetic relationships. S. Dublin isolates (n = 140) were selected from submissions to the NVSL for Salmonella serotyping (2014-2017) from 21 states. Isolates were tested for susceptibility against 14 class-representative antimicrobial drugs. Resistance profiles were determined using the ABRicate with Resfinder and NCBI databases, AMRFinder and PointFinder. Plasmids were detected using ABRicate with PlasmidFinder. Phylogeny was determined using vSNP. We found 98% of the isolates were resistant to more than 4 antimicrobials. Only 1 isolate was pan-susceptible and had no predicted AMR genes. All S. Dublin isolates were susceptible to azithromycin and meropenem. They showed 96% resistance to sulfonamides, 97% to tetracyclines, 95% to aminoglycosides and 85% to beta-lactams. The most common AMR genes were: sulf2 and tetA (98.6%), aph(6)-Id (97.9%), aph(3'')-Ib, (97.1%), floR (94.3%), and blaCMY-2 (85.7%). All quinolone resistant isolates presented mutations in gyrA. Ten plasmid types were identified among all isolates with IncA/C2, IncX1, and IncFII(S) being the most frequent. The S. Dublin isolates show low genomic genetic diversity. This study provided antimicrobial susceptibility and genomic insight into S. Dublin clinical isolates from cattle in the U.S. Further sequence analysis integrating food and human origin S. Dublin isolates may provide valuable insight on increased virulence observed in humans.Mariela E SrednikKristina LantzJessica A HicksBrenda R Morningstar-ShawTonya A MackieLinda K SchlaterPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0249617 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mariela E Srednik Kristina Lantz Jessica A Hicks Brenda R Morningstar-Shaw Tonya A Mackie Linda K Schlater Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States.
description	Salmonella enterica subspecies enterica serotype Dublin is a host-adapted serotype in cattle, associated with enteritis and systemic disease. The primary clinical manifestation of Salmonella Dublin infection in cattle, especially calves, is respiratory disease. While rare in humans, it can cause severe illness, including bacteremia, with hospitalization and death. In the United States, S. Dublin has become one of the most multidrug-resistant serotypes. The objective of this study was to characterize S. Dublin isolates from sick cattle by analyzing phenotypic and genotypic antimicrobial resistance (AMR) profiles, the presence of plasmids, and phylogenetic relationships. S. Dublin isolates (n = 140) were selected from submissions to the NVSL for Salmonella serotyping (2014-2017) from 21 states. Isolates were tested for susceptibility against 14 class-representative antimicrobial drugs. Resistance profiles were determined using the ABRicate with Resfinder and NCBI databases, AMRFinder and PointFinder. Plasmids were detected using ABRicate with PlasmidFinder. Phylogeny was determined using vSNP. We found 98% of the isolates were resistant to more than 4 antimicrobials. Only 1 isolate was pan-susceptible and had no predicted AMR genes. All S. Dublin isolates were susceptible to azithromycin and meropenem. They showed 96% resistance to sulfonamides, 97% to tetracyclines, 95% to aminoglycosides and 85% to beta-lactams. The most common AMR genes were: sulf2 and tetA (98.6%), aph(6)-Id (97.9%), aph(3'')-Ib, (97.1%), floR (94.3%), and blaCMY-2 (85.7%). All quinolone resistant isolates presented mutations in gyrA. Ten plasmid types were identified among all isolates with IncA/C2, IncX1, and IncFII(S) being the most frequent. The S. Dublin isolates show low genomic genetic diversity. This study provided antimicrobial susceptibility and genomic insight into S. Dublin clinical isolates from cattle in the U.S. Further sequence analysis integrating food and human origin S. Dublin isolates may provide valuable insight on increased virulence observed in humans.
format	article
author	Mariela E Srednik Kristina Lantz Jessica A Hicks Brenda R Morningstar-Shaw Tonya A Mackie Linda K Schlater
author_facet	Mariela E Srednik Kristina Lantz Jessica A Hicks Brenda R Morningstar-Shaw Tonya A Mackie Linda K Schlater
author_sort	Mariela E Srednik
title	Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States.
title_short	Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States.
title_full	Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States.
title_fullStr	Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States.
title_full_unstemmed	Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States.
title_sort	antimicrobial resistance and genomic characterization of salmonella dublin isolates in cattle from the united states.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f2fdeda4b687467f8a963b94c19861d2
work_keys_str_mv	AT marielaesrednik antimicrobialresistanceandgenomiccharacterizationofsalmonelladublinisolatesincattlefromtheunitedstates AT kristinalantz antimicrobialresistanceandgenomiccharacterizationofsalmonelladublinisolatesincattlefromtheunitedstates AT jessicaahicks antimicrobialresistanceandgenomiccharacterizationofsalmonelladublinisolatesincattlefromtheunitedstates AT brendarmorningstarshaw antimicrobialresistanceandgenomiccharacterizationofsalmonelladublinisolatesincattlefromtheunitedstates AT tonyaamackie antimicrobialresistanceandgenomiccharacterizationofsalmonelladublinisolatesincattlefromtheunitedstates AT lindakschlater antimicrobialresistanceandgenomiccharacterizationofsalmonelladublinisolatesincattlefromtheunitedstates
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Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States.

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