A novel bone-thinning technique for transcranial stimulation motor-evoked potentials in rats

Abstract Transcranial electrical stimulated motor-evoked potentials (tcMEPs) are widely used to evaluate motor function in humans, and even in animal studies, tcMEPs are used to evaluate neurological dysfunction. However, there is a dearth of reports on extended tcMEP recordings in both animal model...

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Autores principales:	Yuyo Maeda, Takashi Otsuka, Takafumi Mitsuhara, Takahito Okazaki, Louis Yuge, Masaaki Takeda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f2fea02302f44e57a00740b65ecd128e
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spelling	oai:doaj.org-article:f2fea02302f44e57a00740b65ecd128e2021-12-02T17:41:10ZA novel bone-thinning technique for transcranial stimulation motor-evoked potentials in rats10.1038/s41598-021-91780-52045-2322https://doaj.org/article/f2fea02302f44e57a00740b65ecd128e2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91780-5https://doaj.org/toc/2045-2322Abstract Transcranial electrical stimulated motor-evoked potentials (tcMEPs) are widely used to evaluate motor function in humans, and even in animal studies, tcMEPs are used to evaluate neurological dysfunction. However, there is a dearth of reports on extended tcMEP recordings in both animal models and humans. Therefore, this study examined a new technique for stably recording tcMEPs over several weeks in six healthy female Sprague–Dawley rats. We thinned the skull bone using the skull base and spinal surgery technique to reduce electrical resistance for electrical stimulation. tcMEPs were recorded on days 1, 7, 14, 21, and 28 after surgery. The onset latency and amplitude of tcMEPs from the hindlimbs were recorded and evaluated, and histological analysis was performed. Stable amplitude and onset latency could be recorded over several weeks, and histological analysis indicated no complications attributable to the procedure. Thus, our novel technique allows for less invasive, safer, easier, and more stable extended tcMEP recordings than previously reported techniques. The presently reported technique may be applied to the study of various nerve injury models in rats: specifically, to evaluate the degree of nerve dysfunction and recovery in spinal cord injury, cerebral infarction, and brain contusion models.Yuyo MaedaTakashi OtsukaTakafumi MitsuharaTakahito OkazakiLouis YugeMasaaki TakedaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuyo Maeda Takashi Otsuka Takafumi Mitsuhara Takahito Okazaki Louis Yuge Masaaki Takeda A novel bone-thinning technique for transcranial stimulation motor-evoked potentials in rats
description	Abstract Transcranial electrical stimulated motor-evoked potentials (tcMEPs) are widely used to evaluate motor function in humans, and even in animal studies, tcMEPs are used to evaluate neurological dysfunction. However, there is a dearth of reports on extended tcMEP recordings in both animal models and humans. Therefore, this study examined a new technique for stably recording tcMEPs over several weeks in six healthy female Sprague–Dawley rats. We thinned the skull bone using the skull base and spinal surgery technique to reduce electrical resistance for electrical stimulation. tcMEPs were recorded on days 1, 7, 14, 21, and 28 after surgery. The onset latency and amplitude of tcMEPs from the hindlimbs were recorded and evaluated, and histological analysis was performed. Stable amplitude and onset latency could be recorded over several weeks, and histological analysis indicated no complications attributable to the procedure. Thus, our novel technique allows for less invasive, safer, easier, and more stable extended tcMEP recordings than previously reported techniques. The presently reported technique may be applied to the study of various nerve injury models in rats: specifically, to evaluate the degree of nerve dysfunction and recovery in spinal cord injury, cerebral infarction, and brain contusion models.
format	article
author	Yuyo Maeda Takashi Otsuka Takafumi Mitsuhara Takahito Okazaki Louis Yuge Masaaki Takeda
author_facet	Yuyo Maeda Takashi Otsuka Takafumi Mitsuhara Takahito Okazaki Louis Yuge Masaaki Takeda
author_sort	Yuyo Maeda
title	A novel bone-thinning technique for transcranial stimulation motor-evoked potentials in rats
title_short	A novel bone-thinning technique for transcranial stimulation motor-evoked potentials in rats
title_full	A novel bone-thinning technique for transcranial stimulation motor-evoked potentials in rats
title_fullStr	A novel bone-thinning technique for transcranial stimulation motor-evoked potentials in rats
title_full_unstemmed	A novel bone-thinning technique for transcranial stimulation motor-evoked potentials in rats
title_sort	novel bone-thinning technique for transcranial stimulation motor-evoked potentials in rats
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f2fea02302f44e57a00740b65ecd128e
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A novel bone-thinning technique for transcranial stimulation motor-evoked potentials in rats

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