The transcriptional activator of the bfp operon in EPEC (PerA) interacts with the RNA polymerase alpha subunit

Abstract Enteropathogenic E. coli virulence genes are under the control of various regulators, one of which is PerA, an AraC/XylS-like regulator. PerA directly promotes its own expression and that of the bfp operon encoding the genes involved in the biogenesis of the bundle-forming pilus (BFP); it a...

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Autores principales:	Cristina Lara-Ochoa, Fabiola González-Lara, Luis E. Romero-González, Juan B. Jaramillo-Rodríguez, Sergio I. Vázquez-Arellano, Abraham Medrano-López, Lilia Cedillo-Ramírez, Ygnacio Martínez-Laguna, Jorge A. Girón, Ernesto Pérez-Rueda, José Luis Puente, J. Antonio Ibarra
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f3043a289ee647efa797cb6b0aca6621
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spelling	oai:doaj.org-article:f3043a289ee647efa797cb6b0aca66212021-12-02T18:27:49ZThe transcriptional activator of the bfp operon in EPEC (PerA) interacts with the RNA polymerase alpha subunit10.1038/s41598-021-87586-02045-2322https://doaj.org/article/f3043a289ee647efa797cb6b0aca66212021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87586-0https://doaj.org/toc/2045-2322Abstract Enteropathogenic E. coli virulence genes are under the control of various regulators, one of which is PerA, an AraC/XylS-like regulator. PerA directly promotes its own expression and that of the bfp operon encoding the genes involved in the biogenesis of the bundle-forming pilus (BFP); it also activates PerC expression, which in turn stimulates locus of enterocyte effacement (LEE) activation through the LEE-encoded regulator Ler. Monomeric PerA directly binds to the per and bfp regulatory regions; however, it is not known whether interactions between PerA and the RNA polymerase (RNAP) are needed to activate gene transcription as has been observed for other AraC-like regulators. Results showed that PerA interacts with the alpha subunit of the RNAP polymerase and that it is necessary for the genetic and phenotypic expression of bfpA. Furthermore, an in silico analysis shows that PerA might be interacting with specific alpha subunit amino acids residues highlighting the direction of future experiments.Cristina Lara-OchoaFabiola González-LaraLuis E. Romero-GonzálezJuan B. Jaramillo-RodríguezSergio I. Vázquez-ArellanoAbraham Medrano-LópezLilia Cedillo-RamírezYgnacio Martínez-LagunaJorge A. GirónErnesto Pérez-RuedaJosé Luis PuenteJ. Antonio IbarraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cristina Lara-Ochoa Fabiola González-Lara Luis E. Romero-González Juan B. Jaramillo-Rodríguez Sergio I. Vázquez-Arellano Abraham Medrano-López Lilia Cedillo-Ramírez Ygnacio Martínez-Laguna Jorge A. Girón Ernesto Pérez-Rueda José Luis Puente J. Antonio Ibarra The transcriptional activator of the bfp operon in EPEC (PerA) interacts with the RNA polymerase alpha subunit
description	Abstract Enteropathogenic E. coli virulence genes are under the control of various regulators, one of which is PerA, an AraC/XylS-like regulator. PerA directly promotes its own expression and that of the bfp operon encoding the genes involved in the biogenesis of the bundle-forming pilus (BFP); it also activates PerC expression, which in turn stimulates locus of enterocyte effacement (LEE) activation through the LEE-encoded regulator Ler. Monomeric PerA directly binds to the per and bfp regulatory regions; however, it is not known whether interactions between PerA and the RNA polymerase (RNAP) are needed to activate gene transcription as has been observed for other AraC-like regulators. Results showed that PerA interacts with the alpha subunit of the RNAP polymerase and that it is necessary for the genetic and phenotypic expression of bfpA. Furthermore, an in silico analysis shows that PerA might be interacting with specific alpha subunit amino acids residues highlighting the direction of future experiments.
format	article
author	Cristina Lara-Ochoa Fabiola González-Lara Luis E. Romero-González Juan B. Jaramillo-Rodríguez Sergio I. Vázquez-Arellano Abraham Medrano-López Lilia Cedillo-Ramírez Ygnacio Martínez-Laguna Jorge A. Girón Ernesto Pérez-Rueda José Luis Puente J. Antonio Ibarra
author_facet	Cristina Lara-Ochoa Fabiola González-Lara Luis E. Romero-González Juan B. Jaramillo-Rodríguez Sergio I. Vázquez-Arellano Abraham Medrano-López Lilia Cedillo-Ramírez Ygnacio Martínez-Laguna Jorge A. Girón Ernesto Pérez-Rueda José Luis Puente J. Antonio Ibarra
author_sort	Cristina Lara-Ochoa
title	The transcriptional activator of the bfp operon in EPEC (PerA) interacts with the RNA polymerase alpha subunit
title_short	The transcriptional activator of the bfp operon in EPEC (PerA) interacts with the RNA polymerase alpha subunit
title_full	The transcriptional activator of the bfp operon in EPEC (PerA) interacts with the RNA polymerase alpha subunit
title_fullStr	The transcriptional activator of the bfp operon in EPEC (PerA) interacts with the RNA polymerase alpha subunit
title_full_unstemmed	The transcriptional activator of the bfp operon in EPEC (PerA) interacts with the RNA polymerase alpha subunit
title_sort	transcriptional activator of the bfp operon in epec (pera) interacts with the rna polymerase alpha subunit
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f3043a289ee647efa797cb6b0aca6621
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The transcriptional activator of the bfp operon in EPEC (PerA) interacts with the RNA polymerase alpha subunit

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