Differential cohesin loading marks paired and unpaired regions of platypus sex chromosomes at prophase I

Abstract Cohesins are vital for chromosome organisation during meiosis and mitosis. In addition to the important function in sister chromatid cohesion, these complexes play key roles in meiotic recombination, DSB repair, homologous chromosome pairing and segregation. Egg-laying mammals (monotremes)...

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Autores principales:	Aaron E. Casey, Tasman J. Daish, Jose Luis Barbero, Frank Grützner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f309414e94e64661a206fc85982a2a5d
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spelling	oai:doaj.org-article:f309414e94e64661a206fc85982a2a5d2021-12-02T16:08:09ZDifferential cohesin loading marks paired and unpaired regions of platypus sex chromosomes at prophase I10.1038/s41598-017-04560-52045-2322https://doaj.org/article/f309414e94e64661a206fc85982a2a5d2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04560-5https://doaj.org/toc/2045-2322Abstract Cohesins are vital for chromosome organisation during meiosis and mitosis. In addition to the important function in sister chromatid cohesion, these complexes play key roles in meiotic recombination, DSB repair, homologous chromosome pairing and segregation. Egg-laying mammals (monotremes) feature an unusually complex sex chromosome system, which raises fundamental questions about organisation and segregation during meiosis. We discovered a dynamic and differential accumulation of cohesins on sex chromosomes during platypus prophase I and specific reorganisation of the sex chromosome complex around a large nucleolar body. Detailed analysis revealed a differential loading of SMC3 on the chromatin and chromosomal axis of XY shared regions compared with the chromatin and chromosomal axes of asynapsed X and Y regions during prophase I. At late prophase I, SMC3 accumulation is lost from both the chromatin and chromosome axes of the asynaptic regions of the chain and resolves into subnuclear compartments. This is the first report detailing unpaired DNA specific SMC3 accumulation during meiosis in any species and allows speculation on roles for cohesin in monotreme sex chromosome organisation and segregation.Aaron E. CaseyTasman J. DaishJose Luis BarberoFrank GrütznerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aaron E. Casey Tasman J. Daish Jose Luis Barbero Frank Grützner Differential cohesin loading marks paired and unpaired regions of platypus sex chromosomes at prophase I
description	Abstract Cohesins are vital for chromosome organisation during meiosis and mitosis. In addition to the important function in sister chromatid cohesion, these complexes play key roles in meiotic recombination, DSB repair, homologous chromosome pairing and segregation. Egg-laying mammals (monotremes) feature an unusually complex sex chromosome system, which raises fundamental questions about organisation and segregation during meiosis. We discovered a dynamic and differential accumulation of cohesins on sex chromosomes during platypus prophase I and specific reorganisation of the sex chromosome complex around a large nucleolar body. Detailed analysis revealed a differential loading of SMC3 on the chromatin and chromosomal axis of XY shared regions compared with the chromatin and chromosomal axes of asynapsed X and Y regions during prophase I. At late prophase I, SMC3 accumulation is lost from both the chromatin and chromosome axes of the asynaptic regions of the chain and resolves into subnuclear compartments. This is the first report detailing unpaired DNA specific SMC3 accumulation during meiosis in any species and allows speculation on roles for cohesin in monotreme sex chromosome organisation and segregation.
format	article
author	Aaron E. Casey Tasman J. Daish Jose Luis Barbero Frank Grützner
author_facet	Aaron E. Casey Tasman J. Daish Jose Luis Barbero Frank Grützner
author_sort	Aaron E. Casey
title	Differential cohesin loading marks paired and unpaired regions of platypus sex chromosomes at prophase I
title_short	Differential cohesin loading marks paired and unpaired regions of platypus sex chromosomes at prophase I
title_full	Differential cohesin loading marks paired and unpaired regions of platypus sex chromosomes at prophase I
title_fullStr	Differential cohesin loading marks paired and unpaired regions of platypus sex chromosomes at prophase I
title_full_unstemmed	Differential cohesin loading marks paired and unpaired regions of platypus sex chromosomes at prophase I
title_sort	differential cohesin loading marks paired and unpaired regions of platypus sex chromosomes at prophase i
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f309414e94e64661a206fc85982a2a5d
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Differential cohesin loading marks paired and unpaired regions of platypus sex chromosomes at prophase I

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