Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d

Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d

Cas13d is a class 2 type VI-D CRISPR-Cas RNA-guided RNase. Here the authors present the high-resolution crystal structure of the uncultured Ruminococcus sp. Cas13d (UrCas13d)-crRNA complex and by combining structural, mutational and biochemical studies provide mechanistic insights into the CRISPR-Ca...

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Autores principales: Bo Zhang, Yangmiao Ye, Weiwei Ye, Vanja Perčulija, Han Jiang, Yiyang Chen, Yu Li, Jing Chen, Jinying Lin, Siqi Wang, Qi Chen, Yu-San Han, Songying Ouyang
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/f30e478b0a24490ca6dcfdd16c1b148d
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Sumario:Cas13d is a class 2 type VI-D CRISPR-Cas RNA-guided RNase. Here the authors present the high-resolution crystal structure of the uncultured Ruminococcus sp. Cas13d (UrCas13d)-crRNA complex and by combining structural, mutational and biochemical studies provide mechanistic insights into the CRISPR-Cas13d system.

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