Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d

Cas13d is a class 2 type VI-D CRISPR-Cas RNA-guided RNase. Here the authors present the high-resolution crystal structure of the uncultured Ruminococcus sp. Cas13d (UrCas13d)-crRNA complex and by combining structural, mutational and biochemical studies provide mechanistic insights into the CRISPR-Ca...

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Autores principales:	Bo Zhang, Yangmiao Ye, Weiwei Ye, Vanja Perčulija, Han Jiang, Yiyang Chen, Yu Li, Jing Chen, Jinying Lin, Siqi Wang, Qi Chen, Yu-San Han, Songying Ouyang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/f30e478b0a24490ca6dcfdd16c1b148d
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spelling	oai:doaj.org-article:f30e478b0a24490ca6dcfdd16c1b148d2021-12-02T17:01:29ZTwo HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d10.1038/s41467-019-10507-32041-1723https://doaj.org/article/f30e478b0a24490ca6dcfdd16c1b148d2019-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10507-3https://doaj.org/toc/2041-1723Cas13d is a class 2 type VI-D CRISPR-Cas RNA-guided RNase. Here the authors present the high-resolution crystal structure of the uncultured Ruminococcus sp. Cas13d (UrCas13d)-crRNA complex and by combining structural, mutational and biochemical studies provide mechanistic insights into the CRISPR-Cas13d system.Bo ZhangYangmiao YeWeiwei YeVanja PerčulijaHan JiangYiyang ChenYu LiJing ChenJinying LinSiqi WangQi ChenYu-San HanSongying OuyangNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Bo Zhang Yangmiao Ye Weiwei Ye Vanja Perčulija Han Jiang Yiyang Chen Yu Li Jing Chen Jinying Lin Siqi Wang Qi Chen Yu-San Han Songying Ouyang Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d
description	Cas13d is a class 2 type VI-D CRISPR-Cas RNA-guided RNase. Here the authors present the high-resolution crystal structure of the uncultured Ruminococcus sp. Cas13d (UrCas13d)-crRNA complex and by combining structural, mutational and biochemical studies provide mechanistic insights into the CRISPR-Cas13d system.
format	article
author	Bo Zhang Yangmiao Ye Weiwei Ye Vanja Perčulija Han Jiang Yiyang Chen Yu Li Jing Chen Jinying Lin Siqi Wang Qi Chen Yu-San Han Songying Ouyang
author_facet	Bo Zhang Yangmiao Ye Weiwei Ye Vanja Perčulija Han Jiang Yiyang Chen Yu Li Jing Chen Jinying Lin Siqi Wang Qi Chen Yu-San Han Songying Ouyang
author_sort	Bo Zhang
title	Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d
title_short	Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d
title_full	Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d
title_fullStr	Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d
title_full_unstemmed	Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d
title_sort	two hepn domains dictate crispr rna maturation and target cleavage in cas13d
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/f30e478b0a24490ca6dcfdd16c1b148d
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Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d

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