Genetic diversity of sago palm (Metroxylon sagu) accessions based on plastid cpDNA matK gene as DNA barcoding

Abstract. Abbas B, Tjolli I, Munarti. 2020. Genetic diversity of sago palm (Metroxylon sagu) accessions based on plastid cpDNA matK gene as DNA barcoding. Biodiversitas 21: 219-225. Metroxylon sagu Rottb is one of the plant species including in the Palmae family that deposits starch in the trunk. Sa...

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Autores principales:	Barahima Abbas, IHWAN TJOLLI, MUNARTI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	dna barcoding, genetic diversity, matk gene, plastid cpdna, sago palm Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f310867d666548328451938cb98ab644
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spelling	oai:doaj.org-article:f310867d666548328451938cb98ab6442021-11-21T22:09:24ZGenetic diversity of sago palm (Metroxylon sagu) accessions based on plastid cpDNA matK gene as DNA barcoding1412-033X2085-472210.13057/biodiv/d210128https://doaj.org/article/f310867d666548328451938cb98ab6442019-12-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4780https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Abbas B, Tjolli I, Munarti. 2020. Genetic diversity of sago palm (Metroxylon sagu) accessions based on plastid cpDNA matK gene as DNA barcoding. Biodiversitas 21: 219-225. Metroxylon sagu Rottb is one of the plant species including in the Palmae family that deposits starch in the trunk. Sago palm was reported has high capability to produce a large amount of starch and large variation starch qualities which were mostly influenced by genetic factors. The objectives of this study are revealed sequence chloroplast DNA (cpDNA) associated with matK genes for the genetic diversity identification of sago palm accessions. Plant materials used in the studies were derived from Sago Research Center (SRC) collection. DNA extraction has adopted the procedure of Plant Genomic DNA Mini Kit. Polymerase chain reaction (PCR) was performed by using primer sets of MatK-1RKIM-f and MatK-3FKIM-r. DNA PCR product was sequenced by the 1st Base Asia, Singapore. Results of the study showed that the cpDNA sequence associated with matK genes in the genome of sago palm showed differences among accessions. Molecular diversities of sago palm accessions based on matK gene showed sago palm accessions separated into two genotypes. Genotype-1 incorporated of ten individuals of 15 accessions and genotype-2 incorporated five individuals of 15 accessions. A mutation site and deletion site occurred in the sequences of matK gene of the Genotype-2. Ten of the 15 sequences of the matK gene (belong to Genotype-1) were registered in the GenBank, NCBI as DNA barcoding and authenticity of sago palm germplasm from Papua islands, Indonesia territorials.Barahima AbbasIHWAN TJOLLIMUNARTIMBI & UNS Soloarticledna barcoding, genetic diversity, matk gene, plastid cpdna, sago palmBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 1 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	dna barcoding, genetic diversity, matk gene, plastid cpdna, sago palm Biology (General) QH301-705.5
spellingShingle	dna barcoding, genetic diversity, matk gene, plastid cpdna, sago palm Biology (General) QH301-705.5 Barahima Abbas IHWAN TJOLLI MUNARTI Genetic diversity of sago palm (Metroxylon sagu) accessions based on plastid cpDNA matK gene as DNA barcoding
description	Abstract. Abbas B, Tjolli I, Munarti. 2020. Genetic diversity of sago palm (Metroxylon sagu) accessions based on plastid cpDNA matK gene as DNA barcoding. Biodiversitas 21: 219-225. Metroxylon sagu Rottb is one of the plant species including in the Palmae family that deposits starch in the trunk. Sago palm was reported has high capability to produce a large amount of starch and large variation starch qualities which were mostly influenced by genetic factors. The objectives of this study are revealed sequence chloroplast DNA (cpDNA) associated with matK genes for the genetic diversity identification of sago palm accessions. Plant materials used in the studies were derived from Sago Research Center (SRC) collection. DNA extraction has adopted the procedure of Plant Genomic DNA Mini Kit. Polymerase chain reaction (PCR) was performed by using primer sets of MatK-1RKIM-f and MatK-3FKIM-r. DNA PCR product was sequenced by the 1st Base Asia, Singapore. Results of the study showed that the cpDNA sequence associated with matK genes in the genome of sago palm showed differences among accessions. Molecular diversities of sago palm accessions based on matK gene showed sago palm accessions separated into two genotypes. Genotype-1 incorporated of ten individuals of 15 accessions and genotype-2 incorporated five individuals of 15 accessions. A mutation site and deletion site occurred in the sequences of matK gene of the Genotype-2. Ten of the 15 sequences of the matK gene (belong to Genotype-1) were registered in the GenBank, NCBI as DNA barcoding and authenticity of sago palm germplasm from Papua islands, Indonesia territorials.
format	article
author	Barahima Abbas IHWAN TJOLLI MUNARTI
author_facet	Barahima Abbas IHWAN TJOLLI MUNARTI
author_sort	Barahima Abbas
title	Genetic diversity of sago palm (Metroxylon sagu) accessions based on plastid cpDNA matK gene as DNA barcoding
title_short	Genetic diversity of sago palm (Metroxylon sagu) accessions based on plastid cpDNA matK gene as DNA barcoding
title_full	Genetic diversity of sago palm (Metroxylon sagu) accessions based on plastid cpDNA matK gene as DNA barcoding
title_fullStr	Genetic diversity of sago palm (Metroxylon sagu) accessions based on plastid cpDNA matK gene as DNA barcoding
title_full_unstemmed	Genetic diversity of sago palm (Metroxylon sagu) accessions based on plastid cpDNA matK gene as DNA barcoding
title_sort	genetic diversity of sago palm (metroxylon sagu) accessions based on plastid cpdna matk gene as dna barcoding
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/f310867d666548328451938cb98ab644
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Genetic diversity of sago palm (Metroxylon sagu) accessions based on plastid cpDNA matK gene as DNA barcoding

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