Lipid droplets contribute myogenic differentiation in C2C12 by promoting the remodeling of the acstin-filament

Abstract Lipid droplet (LD), a multi-functional organelle, is found in most eukaryotic cells. LDs participate in the regulation of many cellular processes including proliferation, stress, and apoptosis. Previous studies showed the athlete’s paradox that trained athletes accumulate LDs in their skele...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yanjie Tan, Yi Jin, Pengxiang Zhao, Jian Wu, Zhuqing Ren
Formato:	article
Lenguaje:	EN
Publicado:	Nature Publishing Group 2021
Materias:	Cytology QH573-671
Acceso en línea:	https://doaj.org/article/f3166beb05904d37b8e96a0d1eb6e8c8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f3166beb05904d37b8e96a0d1eb6e8c8
record_format	dspace
spelling	oai:doaj.org-article:f3166beb05904d37b8e96a0d1eb6e8c82021-11-28T12:04:18ZLipid droplets contribute myogenic differentiation in C2C12 by promoting the remodeling of the acstin-filament10.1038/s41419-021-04273-82041-4889https://doaj.org/article/f3166beb05904d37b8e96a0d1eb6e8c82021-11-01T00:00:00Zhttps://doi.org/10.1038/s41419-021-04273-8https://doaj.org/toc/2041-4889Abstract Lipid droplet (LD), a multi-functional organelle, is found in most eukaryotic cells. LDs participate in the regulation of many cellular processes including proliferation, stress, and apoptosis. Previous studies showed the athlete’s paradox that trained athletes accumulate LDs in their skeletal muscle. However, the impact of LDs on skeletal muscle and myogenesis is not clear. We discovered that C2C12 myoblast cells containing more LDs formed more multinucleated muscle fibers. We also discovered that LDs promoted cell migration and fusion by promoting actin-filaments remodeling. Mechanistically, two LD-proteins, Acyl-CoA synthetase long chain family member 3 (ACSL3) and lysophosphatidylcholine acyltransferase 1 (LPCAT1), medicated the recruitment of actinin proteins which contributed to actin-filaments formation on the surface of LDs. During remodeling, the actinin proteins on LDs surface translocated to actin-filaments via ARF1/COPI vesicles. Our study demonstrate LDs contribute to cell differentiation, which lead to new insight into the LD function.Yanjie TanYi JinPengxiang ZhaoJian WuZhuqing RenNature Publishing GrouparticleCytologyQH573-671ENCell Death and Disease, Vol 12, Iss 12, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cytology QH573-671
spellingShingle	Cytology QH573-671 Yanjie Tan Yi Jin Pengxiang Zhao Jian Wu Zhuqing Ren Lipid droplets contribute myogenic differentiation in C2C12 by promoting the remodeling of the acstin-filament
description	Abstract Lipid droplet (LD), a multi-functional organelle, is found in most eukaryotic cells. LDs participate in the regulation of many cellular processes including proliferation, stress, and apoptosis. Previous studies showed the athlete’s paradox that trained athletes accumulate LDs in their skeletal muscle. However, the impact of LDs on skeletal muscle and myogenesis is not clear. We discovered that C2C12 myoblast cells containing more LDs formed more multinucleated muscle fibers. We also discovered that LDs promoted cell migration and fusion by promoting actin-filaments remodeling. Mechanistically, two LD-proteins, Acyl-CoA synthetase long chain family member 3 (ACSL3) and lysophosphatidylcholine acyltransferase 1 (LPCAT1), medicated the recruitment of actinin proteins which contributed to actin-filaments formation on the surface of LDs. During remodeling, the actinin proteins on LDs surface translocated to actin-filaments via ARF1/COPI vesicles. Our study demonstrate LDs contribute to cell differentiation, which lead to new insight into the LD function.
format	article
author	Yanjie Tan Yi Jin Pengxiang Zhao Jian Wu Zhuqing Ren
author_facet	Yanjie Tan Yi Jin Pengxiang Zhao Jian Wu Zhuqing Ren
author_sort	Yanjie Tan
title	Lipid droplets contribute myogenic differentiation in C2C12 by promoting the remodeling of the acstin-filament
title_short	Lipid droplets contribute myogenic differentiation in C2C12 by promoting the remodeling of the acstin-filament
title_full	Lipid droplets contribute myogenic differentiation in C2C12 by promoting the remodeling of the acstin-filament
title_fullStr	Lipid droplets contribute myogenic differentiation in C2C12 by promoting the remodeling of the acstin-filament
title_full_unstemmed	Lipid droplets contribute myogenic differentiation in C2C12 by promoting the remodeling of the acstin-filament
title_sort	lipid droplets contribute myogenic differentiation in c2c12 by promoting the remodeling of the acstin-filament
publisher	Nature Publishing Group
publishDate	2021
url	https://doaj.org/article/f3166beb05904d37b8e96a0d1eb6e8c8
work_keys_str_mv	AT yanjietan lipiddropletscontributemyogenicdifferentiationinc2c12bypromotingtheremodelingoftheacstinfilament AT yijin lipiddropletscontributemyogenicdifferentiationinc2c12bypromotingtheremodelingoftheacstinfilament AT pengxiangzhao lipiddropletscontributemyogenicdifferentiationinc2c12bypromotingtheremodelingoftheacstinfilament AT jianwu lipiddropletscontributemyogenicdifferentiationinc2c12bypromotingtheremodelingoftheacstinfilament AT zhuqingren lipiddropletscontributemyogenicdifferentiationinc2c12bypromotingtheremodelingoftheacstinfilament
_version_	1718408234269671424

Lipid droplets contribute myogenic differentiation in C2C12 by promoting the remodeling of the acstin-filament

Ejemplares similares