Newly developed Mg2+-selective fluorescent probe enables visualization of Mg2+ dynamics in mitochondria.

Mg(2+) plays important roles in numerous cellular functions. Mitochondria take part in intracellular Mg(2+) regulation and the Mg(2+) concentration in mitochondria affects the synthesis of ATP. However, there are few methods to observe Mg(2+) in mitochondria in intact cells. Here, we have developed...

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Autores principales:	Yutaka Shindo, Tomohiko Fujii, Hirokazu Komatsu, Daniel Citterio, Kohji Hotta, Koji Suzuki, Kotaro Oka
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f316dba6dca54b6c839af313e575728a
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spelling	oai:doaj.org-article:f316dba6dca54b6c839af313e575728a2021-11-18T06:47:52ZNewly developed Mg2+-selective fluorescent probe enables visualization of Mg2+ dynamics in mitochondria.1932-620310.1371/journal.pone.0023684https://doaj.org/article/f316dba6dca54b6c839af313e575728a2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21858208/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Mg(2+) plays important roles in numerous cellular functions. Mitochondria take part in intracellular Mg(2+) regulation and the Mg(2+) concentration in mitochondria affects the synthesis of ATP. However, there are few methods to observe Mg(2+) in mitochondria in intact cells. Here, we have developed a novel Mg(2+)-selective fluorescent probe, KMG-301, that is functional in mitochondria. This probe changes its fluorescence properties solely depending on the Mg(2+) concentration in mitochondria under physiologically normal conditions. Simultaneous measurements using this probe together with a probe for cytosolic Mg(2+), KMG-104, enabled us to compare the dynamics of Mg(2+) in the cytosol and in mitochondria. With this method, carbonyl cyanide p-(trifluoromethoxy) phenylhydrazone (FCCP)-induced Mg(2+) mobilization from mitochondria to the cytosol was visualized. Although a FCCP-induced decrease in the Mg(2+) concentration in mitochondria and an increase in the cytosol were observed both in differentiated PC12 cells and in hippocampal neurons, the time-courses of concentration changes varied with cell type. Moreover, the relationship between mitochondrial Mg(2+) and Parkinson's disease was analyzed in a cellular model of Parkinson's disease by using the 1-methyl-4-phenylpyridinium ion (MPP(+)). A gradual decrease in the Mg(2+) concentration in mitochondria was observed in response to MPP(+) in differentiated PC12 cells. These results indicate that KMG-301 is useful for investigating Mg(2+) dynamics in mitochondria. All animal procedures to obtain neurons from Wistar rats were approved by the ethical committee of Keio University (permit number is 09106-(1)).Yutaka ShindoTomohiko FujiiHirokazu KomatsuDaniel CitterioKohji HottaKoji SuzukiKotaro OkaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 8, p e23684 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yutaka Shindo Tomohiko Fujii Hirokazu Komatsu Daniel Citterio Kohji Hotta Koji Suzuki Kotaro Oka Newly developed Mg2+-selective fluorescent probe enables visualization of Mg2+ dynamics in mitochondria.
description	Mg(2+) plays important roles in numerous cellular functions. Mitochondria take part in intracellular Mg(2+) regulation and the Mg(2+) concentration in mitochondria affects the synthesis of ATP. However, there are few methods to observe Mg(2+) in mitochondria in intact cells. Here, we have developed a novel Mg(2+)-selective fluorescent probe, KMG-301, that is functional in mitochondria. This probe changes its fluorescence properties solely depending on the Mg(2+) concentration in mitochondria under physiologically normal conditions. Simultaneous measurements using this probe together with a probe for cytosolic Mg(2+), KMG-104, enabled us to compare the dynamics of Mg(2+) in the cytosol and in mitochondria. With this method, carbonyl cyanide p-(trifluoromethoxy) phenylhydrazone (FCCP)-induced Mg(2+) mobilization from mitochondria to the cytosol was visualized. Although a FCCP-induced decrease in the Mg(2+) concentration in mitochondria and an increase in the cytosol were observed both in differentiated PC12 cells and in hippocampal neurons, the time-courses of concentration changes varied with cell type. Moreover, the relationship between mitochondrial Mg(2+) and Parkinson's disease was analyzed in a cellular model of Parkinson's disease by using the 1-methyl-4-phenylpyridinium ion (MPP(+)). A gradual decrease in the Mg(2+) concentration in mitochondria was observed in response to MPP(+) in differentiated PC12 cells. These results indicate that KMG-301 is useful for investigating Mg(2+) dynamics in mitochondria. All animal procedures to obtain neurons from Wistar rats were approved by the ethical committee of Keio University (permit number is 09106-(1)).
format	article
author	Yutaka Shindo Tomohiko Fujii Hirokazu Komatsu Daniel Citterio Kohji Hotta Koji Suzuki Kotaro Oka
author_facet	Yutaka Shindo Tomohiko Fujii Hirokazu Komatsu Daniel Citterio Kohji Hotta Koji Suzuki Kotaro Oka
author_sort	Yutaka Shindo
title	Newly developed Mg2+-selective fluorescent probe enables visualization of Mg2+ dynamics in mitochondria.
title_short	Newly developed Mg2+-selective fluorescent probe enables visualization of Mg2+ dynamics in mitochondria.
title_full	Newly developed Mg2+-selective fluorescent probe enables visualization of Mg2+ dynamics in mitochondria.
title_fullStr	Newly developed Mg2+-selective fluorescent probe enables visualization of Mg2+ dynamics in mitochondria.
title_full_unstemmed	Newly developed Mg2+-selective fluorescent probe enables visualization of Mg2+ dynamics in mitochondria.
title_sort	newly developed mg2+-selective fluorescent probe enables visualization of mg2+ dynamics in mitochondria.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/f316dba6dca54b6c839af313e575728a
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Newly developed Mg2+-selective fluorescent probe enables visualization of Mg2+ dynamics in mitochondria.

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