Inter-MAR association contributes to transcriptionally active looping events in human beta-globin gene cluster.

Matrix attachment regions (MARs) are important in chromatin organization and gene regulation. Although it is known that there are a number of MAR elements in the beta-globin gene cluster, it is unclear that how these MAR elements are involved in regulating beta-globin genes expression. Here, we repo...

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Autores principales:	Li Wang, Li-Jun Di, Xiang Lv, Wei Zheng, Zheng Xue, Zhi-Chen Guo, De-Pei Liu, Chi-Chuan Liang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f31a1751681e44189fa7f5978f43533a
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spelling	oai:doaj.org-article:f31a1751681e44189fa7f5978f43533a2021-11-25T06:17:01ZInter-MAR association contributes to transcriptionally active looping events in human beta-globin gene cluster.1932-620310.1371/journal.pone.0004629https://doaj.org/article/f31a1751681e44189fa7f5978f43533a2009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19247486/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Matrix attachment regions (MARs) are important in chromatin organization and gene regulation. Although it is known that there are a number of MAR elements in the beta-globin gene cluster, it is unclear that how these MAR elements are involved in regulating beta-globin genes expression. Here, we report the identification of a new MAR element at the LCR (locus control region) of human beta-globin gene cluster and the detection of the inter-MAR association within the beta-globin gene cluster. Also, we demonstrate that SATB1, a protein factor that has been implicated in the formation of network like higher order chromatin structures at some gene loci, takes part in beta-globin specific inter-MAR association through binding the specific MARs. Knocking down of SATB1 obviously reduces the binding of SATB1 to the MARs and diminishes the frequency of the inter-MAR association. As a result, the ACH establishment and the alpha-like globin genes and beta-like globin genes expressions are affected either. In summary, our results suggest that SATB1 is a regulatory factor of hemoglobin genes, especially the early differentiation genes at least through affecting the higher order chromatin structure.Li WangLi-Jun DiXiang LvWei ZhengZheng XueZhi-Chen GuoDe-Pei LiuChi-Chuan LiangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 2, p e4629 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Li Wang Li-Jun Di Xiang Lv Wei Zheng Zheng Xue Zhi-Chen Guo De-Pei Liu Chi-Chuan Liang Inter-MAR association contributes to transcriptionally active looping events in human beta-globin gene cluster.
description	Matrix attachment regions (MARs) are important in chromatin organization and gene regulation. Although it is known that there are a number of MAR elements in the beta-globin gene cluster, it is unclear that how these MAR elements are involved in regulating beta-globin genes expression. Here, we report the identification of a new MAR element at the LCR (locus control region) of human beta-globin gene cluster and the detection of the inter-MAR association within the beta-globin gene cluster. Also, we demonstrate that SATB1, a protein factor that has been implicated in the formation of network like higher order chromatin structures at some gene loci, takes part in beta-globin specific inter-MAR association through binding the specific MARs. Knocking down of SATB1 obviously reduces the binding of SATB1 to the MARs and diminishes the frequency of the inter-MAR association. As a result, the ACH establishment and the alpha-like globin genes and beta-like globin genes expressions are affected either. In summary, our results suggest that SATB1 is a regulatory factor of hemoglobin genes, especially the early differentiation genes at least through affecting the higher order chromatin structure.
format	article
author	Li Wang Li-Jun Di Xiang Lv Wei Zheng Zheng Xue Zhi-Chen Guo De-Pei Liu Chi-Chuan Liang
author_facet	Li Wang Li-Jun Di Xiang Lv Wei Zheng Zheng Xue Zhi-Chen Guo De-Pei Liu Chi-Chuan Liang
author_sort	Li Wang
title	Inter-MAR association contributes to transcriptionally active looping events in human beta-globin gene cluster.
title_short	Inter-MAR association contributes to transcriptionally active looping events in human beta-globin gene cluster.
title_full	Inter-MAR association contributes to transcriptionally active looping events in human beta-globin gene cluster.
title_fullStr	Inter-MAR association contributes to transcriptionally active looping events in human beta-globin gene cluster.
title_full_unstemmed	Inter-MAR association contributes to transcriptionally active looping events in human beta-globin gene cluster.
title_sort	inter-mar association contributes to transcriptionally active looping events in human beta-globin gene cluster.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/f31a1751681e44189fa7f5978f43533a
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Inter-MAR association contributes to transcriptionally active looping events in human beta-globin gene cluster.

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