Transcriptome Differences in Response Mechanisms to Low-Nitrogen Stress in Two Wheat Varieties
Nitrogen plays a crucial role in wheat growth and development. Here, we analyzed the tolerance of wheat strains XM26 and LM23 to low-nitrogen stress using a chlorate sensitivity experiment. Subsequently, we performed transcriptome analyses of both varieties exposed to low-nitrogen (LN) and normal (C...
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2021
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oai:doaj.org-article:f31adfbcab0e4c6b998bc3d6360ce9842021-11-25T17:54:58ZTranscriptome Differences in Response Mechanisms to Low-Nitrogen Stress in Two Wheat Varieties10.3390/ijms2222122781422-00671661-6596https://doaj.org/article/f31adfbcab0e4c6b998bc3d6360ce9842021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12278https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Nitrogen plays a crucial role in wheat growth and development. Here, we analyzed the tolerance of wheat strains XM26 and LM23 to low-nitrogen stress using a chlorate sensitivity experiment. Subsequently, we performed transcriptome analyses of both varieties exposed to low-nitrogen (LN) and normal (CK) treatments. Compared with those under CK treatment, 3534 differentially expressed genes (DEGs) were detected in XM26 in roots and shoots under LN treatment (<i>p</i> < 0.05, and |log2FC| > 1). A total of 3584 DEGs were detected in LM23. A total of 3306 DEGs, including 863 DEGs in roots and 2443 DEGs in shoots, were specifically expressed in XM26 or showed huge differences between XM26 and LM23 (log2FC ratio > 3). These were selected for gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses. The calcium-mediated plant–pathogen interaction, MAPK signaling, and phosphatidylinositol signaling pathways were enriched in XM26 but not in LM23. We also verified the expression of important genes involved in these pathways in the two varieties using qRT-PCR. A total of 156 transcription factors were identified among the DEGs, and their expression patterns were different between the two varieties. Our findings suggest that calcium-related pathways play different roles in the two varieties, eliciting different tolerances to low-nitrogen stress.Huishu YanHuawei ShiChengmei HuMingzhao LuoChengjie XuShuguang WangNing LiWensi TangYongbin ZhouChunxiao WangZhaoshi XuJun ChenYouzhi MaDaizhen SunMing ChenMDPI AGarticlewheatnitrogen stress tolerancetranscriptomecalciumBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12278, p 12278 (2021) |
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| topic |
wheat nitrogen stress tolerance transcriptome calcium Biology (General) QH301-705.5 Chemistry QD1-999 |
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wheat nitrogen stress tolerance transcriptome calcium Biology (General) QH301-705.5 Chemistry QD1-999 Huishu Yan Huawei Shi Chengmei Hu Mingzhao Luo Chengjie Xu Shuguang Wang Ning Li Wensi Tang Yongbin Zhou Chunxiao Wang Zhaoshi Xu Jun Chen Youzhi Ma Daizhen Sun Ming Chen Transcriptome Differences in Response Mechanisms to Low-Nitrogen Stress in Two Wheat Varieties |
| description |
Nitrogen plays a crucial role in wheat growth and development. Here, we analyzed the tolerance of wheat strains XM26 and LM23 to low-nitrogen stress using a chlorate sensitivity experiment. Subsequently, we performed transcriptome analyses of both varieties exposed to low-nitrogen (LN) and normal (CK) treatments. Compared with those under CK treatment, 3534 differentially expressed genes (DEGs) were detected in XM26 in roots and shoots under LN treatment (<i>p</i> < 0.05, and |log2FC| > 1). A total of 3584 DEGs were detected in LM23. A total of 3306 DEGs, including 863 DEGs in roots and 2443 DEGs in shoots, were specifically expressed in XM26 or showed huge differences between XM26 and LM23 (log2FC ratio > 3). These were selected for gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses. The calcium-mediated plant–pathogen interaction, MAPK signaling, and phosphatidylinositol signaling pathways were enriched in XM26 but not in LM23. We also verified the expression of important genes involved in these pathways in the two varieties using qRT-PCR. A total of 156 transcription factors were identified among the DEGs, and their expression patterns were different between the two varieties. Our findings suggest that calcium-related pathways play different roles in the two varieties, eliciting different tolerances to low-nitrogen stress. |
| format |
article |
| author |
Huishu Yan Huawei Shi Chengmei Hu Mingzhao Luo Chengjie Xu Shuguang Wang Ning Li Wensi Tang Yongbin Zhou Chunxiao Wang Zhaoshi Xu Jun Chen Youzhi Ma Daizhen Sun Ming Chen |
| author_facet |
Huishu Yan Huawei Shi Chengmei Hu Mingzhao Luo Chengjie Xu Shuguang Wang Ning Li Wensi Tang Yongbin Zhou Chunxiao Wang Zhaoshi Xu Jun Chen Youzhi Ma Daizhen Sun Ming Chen |
| author_sort |
Huishu Yan |
| title |
Transcriptome Differences in Response Mechanisms to Low-Nitrogen Stress in Two Wheat Varieties |
| title_short |
Transcriptome Differences in Response Mechanisms to Low-Nitrogen Stress in Two Wheat Varieties |
| title_full |
Transcriptome Differences in Response Mechanisms to Low-Nitrogen Stress in Two Wheat Varieties |
| title_fullStr |
Transcriptome Differences in Response Mechanisms to Low-Nitrogen Stress in Two Wheat Varieties |
| title_full_unstemmed |
Transcriptome Differences in Response Mechanisms to Low-Nitrogen Stress in Two Wheat Varieties |
| title_sort |
transcriptome differences in response mechanisms to low-nitrogen stress in two wheat varieties |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/f31adfbcab0e4c6b998bc3d6360ce984 |
| work_keys_str_mv |
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