Microfluidic Cell Retention Device for Perfusion of Mammalian Suspension Culture

Abstract Continuous production of biologics, a growing trend in the biopharmaceutical industry, requires a reliable and efficient cell retention device that also maintains cell viability. Current filtration methods, such as tangential flow filtration using hollow-fiber membranes, suffer from membran...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Taehong Kwon, Holly Prentice, Jonas De Oliveira, Nyasha Madziva, Majid Ebrahimi Warkiani, Jean-François P. Hamel, Jongyoon Han
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f3245607a2aa4041aa73c8e026a442cd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f3245607a2aa4041aa73c8e026a442cd
record_format	dspace
spelling	oai:doaj.org-article:f3245607a2aa4041aa73c8e026a442cd2021-12-02T11:53:00ZMicrofluidic Cell Retention Device for Perfusion of Mammalian Suspension Culture10.1038/s41598-017-06949-82045-2322https://doaj.org/article/f3245607a2aa4041aa73c8e026a442cd2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06949-8https://doaj.org/toc/2045-2322Abstract Continuous production of biologics, a growing trend in the biopharmaceutical industry, requires a reliable and efficient cell retention device that also maintains cell viability. Current filtration methods, such as tangential flow filtration using hollow-fiber membranes, suffer from membrane fouling, leading to significant reliability and productivity issues such as low cell viability, product retention, and an increased contamination risk associated with filter replacement. We introduce a novel cell retention device based on inertial sorting for perfusion culture of suspended mammalian cells. The device was characterized in terms of cell retention capacity, biocompatibility, scalability, and long-term reliability. This technology was demonstrated using a high concentration (>20 million cells/mL) perfusion culture of an IgG1-producing Chinese hamster ovary (CHO) cell line for 18–25 days. The device demonstrated reliable and clog-free cell retention, high IgG1 recovery (>99%) and cell viability (>97%). Lab-scale perfusion cultures (350 mL) were used to demonstrate the technology, which can be scaled-out with parallel devices to enable larger scale operation. The new cell retention device is thus ideal for rapid perfusion process development in a biomanufacturing workflow.Taehong KwonHolly PrenticeJonas De OliveiraNyasha MadzivaMajid Ebrahimi WarkianiJean-François P. HamelJongyoon HanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Taehong Kwon Holly Prentice Jonas De Oliveira Nyasha Madziva Majid Ebrahimi Warkiani Jean-François P. Hamel Jongyoon Han Microfluidic Cell Retention Device for Perfusion of Mammalian Suspension Culture
description	Abstract Continuous production of biologics, a growing trend in the biopharmaceutical industry, requires a reliable and efficient cell retention device that also maintains cell viability. Current filtration methods, such as tangential flow filtration using hollow-fiber membranes, suffer from membrane fouling, leading to significant reliability and productivity issues such as low cell viability, product retention, and an increased contamination risk associated with filter replacement. We introduce a novel cell retention device based on inertial sorting for perfusion culture of suspended mammalian cells. The device was characterized in terms of cell retention capacity, biocompatibility, scalability, and long-term reliability. This technology was demonstrated using a high concentration (>20 million cells/mL) perfusion culture of an IgG1-producing Chinese hamster ovary (CHO) cell line for 18–25 days. The device demonstrated reliable and clog-free cell retention, high IgG1 recovery (>99%) and cell viability (>97%). Lab-scale perfusion cultures (350 mL) were used to demonstrate the technology, which can be scaled-out with parallel devices to enable larger scale operation. The new cell retention device is thus ideal for rapid perfusion process development in a biomanufacturing workflow.
format	article
author	Taehong Kwon Holly Prentice Jonas De Oliveira Nyasha Madziva Majid Ebrahimi Warkiani Jean-François P. Hamel Jongyoon Han
author_facet	Taehong Kwon Holly Prentice Jonas De Oliveira Nyasha Madziva Majid Ebrahimi Warkiani Jean-François P. Hamel Jongyoon Han
author_sort	Taehong Kwon
title	Microfluidic Cell Retention Device for Perfusion of Mammalian Suspension Culture
title_short	Microfluidic Cell Retention Device for Perfusion of Mammalian Suspension Culture
title_full	Microfluidic Cell Retention Device for Perfusion of Mammalian Suspension Culture
title_fullStr	Microfluidic Cell Retention Device for Perfusion of Mammalian Suspension Culture
title_full_unstemmed	Microfluidic Cell Retention Device for Perfusion of Mammalian Suspension Culture
title_sort	microfluidic cell retention device for perfusion of mammalian suspension culture
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f3245607a2aa4041aa73c8e026a442cd
work_keys_str_mv	AT taehongkwon microfluidiccellretentiondeviceforperfusionofmammaliansuspensionculture AT hollyprentice microfluidiccellretentiondeviceforperfusionofmammaliansuspensionculture AT jonasdeoliveira microfluidiccellretentiondeviceforperfusionofmammaliansuspensionculture AT nyashamadziva microfluidiccellretentiondeviceforperfusionofmammaliansuspensionculture AT majidebrahimiwarkiani microfluidiccellretentiondeviceforperfusionofmammaliansuspensionculture AT jeanfrancoisphamel microfluidiccellretentiondeviceforperfusionofmammaliansuspensionculture AT jongyoonhan microfluidiccellretentiondeviceforperfusionofmammaliansuspensionculture
_version_	1718394961166073856

Microfluidic Cell Retention Device for Perfusion of Mammalian Suspension Culture

Ejemplares similares