Antigenic analysis of monoclonal antibodies against different epitopes of σB protein of avian reovirus.

<h4>Background</h4>Avian reovirus (ARV) causes arthritis, tenosynovitis, runting-stunting syndrome (RSS), malabsorption syndrome (MAS) and immunosuppression in chickens. σB is one of the major structural proteins of ARV, which is able to induce group-specific antibodies against the virus...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chun-hong Yin, Li-ting Qin, Mei-yu Sun, Yu-long Gao, Xiao-le Qi, Hong-lei Gao, Yong-qiang Wang, Xiao-mei Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f33761dde83a4e5eaf033c9652861f23
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f33761dde83a4e5eaf033c9652861f23
record_format	dspace
spelling	oai:doaj.org-article:f33761dde83a4e5eaf033c9652861f232021-11-18T08:44:23ZAntigenic analysis of monoclonal antibodies against different epitopes of σB protein of avian reovirus.1932-620310.1371/journal.pone.0081533https://doaj.org/article/f33761dde83a4e5eaf033c9652861f232013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24312314/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Avian reovirus (ARV) causes arthritis, tenosynovitis, runting-stunting syndrome (RSS), malabsorption syndrome (MAS) and immunosuppression in chickens. σB is one of the major structural proteins of ARV, which is able to induce group-specific antibodies against the virus.<h4>Methods and results</h4>The present study described the identification of two linear B-cell epitopes in ARV σB through expressing a set of partially overlapping and consecutive truncated peptides spanning σB screened with two monoclonal antibodies (mAbs) 1F4 and 1H3-1.The data indicated that (21)KTPACW(26) (epitope A) and (32)WDTVTFH(38) (epitope B) were minimal determinants of the linear B cell epitopes. Antibodies present in the serum of ARV-positive chickens recognized the minimal linear epitopes in Western blot analyses. By sequence alignment analysis, we determined that the epitopes A and B were not conserved among ARV, duck reovirus (DRV) and turkey reovirus (TRV) strains. Western blot assays, confirmed that epitopes A and B were ARV-specific epitopes, and they could not react with the corresponding peptides of DRV and TRV.<h4>Conclusions and significance</h4>We identified (21)KTPACW(26) and (32)WDTVTFH(38) as σB -specific epitopes recognized by mAbs 1F4 and 1H3-1, respectively. The results in this study may have potential applications in development of diagnostic techniques and epitope-based marker vaccines against ARV groups.Chun-hong YinLi-ting QinMei-yu SunYu-long GaoXiao-le QiHong-lei GaoYong-qiang WangXiao-mei WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e81533 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chun-hong Yin Li-ting Qin Mei-yu Sun Yu-long Gao Xiao-le Qi Hong-lei Gao Yong-qiang Wang Xiao-mei Wang Antigenic analysis of monoclonal antibodies against different epitopes of σB protein of avian reovirus.
description	<h4>Background</h4>Avian reovirus (ARV) causes arthritis, tenosynovitis, runting-stunting syndrome (RSS), malabsorption syndrome (MAS) and immunosuppression in chickens. σB is one of the major structural proteins of ARV, which is able to induce group-specific antibodies against the virus.<h4>Methods and results</h4>The present study described the identification of two linear B-cell epitopes in ARV σB through expressing a set of partially overlapping and consecutive truncated peptides spanning σB screened with two monoclonal antibodies (mAbs) 1F4 and 1H3-1.The data indicated that (21)KTPACW(26) (epitope A) and (32)WDTVTFH(38) (epitope B) were minimal determinants of the linear B cell epitopes. Antibodies present in the serum of ARV-positive chickens recognized the minimal linear epitopes in Western blot analyses. By sequence alignment analysis, we determined that the epitopes A and B were not conserved among ARV, duck reovirus (DRV) and turkey reovirus (TRV) strains. Western blot assays, confirmed that epitopes A and B were ARV-specific epitopes, and they could not react with the corresponding peptides of DRV and TRV.<h4>Conclusions and significance</h4>We identified (21)KTPACW(26) and (32)WDTVTFH(38) as σB -specific epitopes recognized by mAbs 1F4 and 1H3-1, respectively. The results in this study may have potential applications in development of diagnostic techniques and epitope-based marker vaccines against ARV groups.
format	article
author	Chun-hong Yin Li-ting Qin Mei-yu Sun Yu-long Gao Xiao-le Qi Hong-lei Gao Yong-qiang Wang Xiao-mei Wang
author_facet	Chun-hong Yin Li-ting Qin Mei-yu Sun Yu-long Gao Xiao-le Qi Hong-lei Gao Yong-qiang Wang Xiao-mei Wang
author_sort	Chun-hong Yin
title	Antigenic analysis of monoclonal antibodies against different epitopes of σB protein of avian reovirus.
title_short	Antigenic analysis of monoclonal antibodies against different epitopes of σB protein of avian reovirus.
title_full	Antigenic analysis of monoclonal antibodies against different epitopes of σB protein of avian reovirus.
title_fullStr	Antigenic analysis of monoclonal antibodies against different epitopes of σB protein of avian reovirus.
title_full_unstemmed	Antigenic analysis of monoclonal antibodies against different epitopes of σB protein of avian reovirus.
title_sort	antigenic analysis of monoclonal antibodies against different epitopes of σb protein of avian reovirus.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/f33761dde83a4e5eaf033c9652861f23
work_keys_str_mv	AT chunhongyin antigenicanalysisofmonoclonalantibodiesagainstdifferentepitopesofsbproteinofavianreovirus AT litingqin antigenicanalysisofmonoclonalantibodiesagainstdifferentepitopesofsbproteinofavianreovirus AT meiyusun antigenicanalysisofmonoclonalantibodiesagainstdifferentepitopesofsbproteinofavianreovirus AT yulonggao antigenicanalysisofmonoclonalantibodiesagainstdifferentepitopesofsbproteinofavianreovirus AT xiaoleqi antigenicanalysisofmonoclonalantibodiesagainstdifferentepitopesofsbproteinofavianreovirus AT hongleigao antigenicanalysisofmonoclonalantibodiesagainstdifferentepitopesofsbproteinofavianreovirus AT yongqiangwang antigenicanalysisofmonoclonalantibodiesagainstdifferentepitopesofsbproteinofavianreovirus AT xiaomeiwang antigenicanalysisofmonoclonalantibodiesagainstdifferentepitopesofsbproteinofavianreovirus
_version_	1718421419167055872

Antigenic analysis of monoclonal antibodies against different epitopes of σB protein of avian reovirus.

Ejemplares similares