Cytotoxicity of five fluoroquinolone and two nonsteroidal anti-inflammatory benzalkonium chloride-free ophthalmic solutions in four corneoconjunctival cell lines

Masahiko Ayaki1, Atsuo Iwasawa2, Mitsutaka Soda3, Shigeo Yaguchi3, Ryohei Koide41Department of Ophthalmology, Saitama National Hospital, Saitama, Japan; 2Department of Clinical Pathology, Showa University Fujigaoka Hospital, Yokohama, Japan; 3Department of Ophthalmology, Showa University Fujigaoka R...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Masahiko Ayaki, Atsuo Iwasawa, Mitsutaka Soda, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/f3389f0b480b44af81d355d135db9b0b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f3389f0b480b44af81d355d135db9b0b
record_format	dspace
spelling	oai:doaj.org-article:f3389f0b480b44af81d355d135db9b0b2021-12-02T00:44:48ZCytotoxicity of five fluoroquinolone and two nonsteroidal anti-inflammatory benzalkonium chloride-free ophthalmic solutions in four corneoconjunctival cell lines1177-54671177-5483https://doaj.org/article/f3389f0b480b44af81d355d135db9b0b2010-09-01T00:00:00Zhttp://www.dovepress.com/cytotoxicity-of-five-fluoroquinolone-and-two-nonsteroidal-anti-inflamm-a5302https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Masahiko Ayaki1, Atsuo Iwasawa2, Mitsutaka Soda3, Shigeo Yaguchi3, Ryohei Koide41Department of Ophthalmology, Saitama National Hospital, Saitama, Japan; 2Department of Clinical Pathology, Showa University Fujigaoka Hospital, Yokohama, Japan; 3Department of Ophthalmology, Showa University Fujigaoka Rehabilitation Hospital, Yokohama, Japan; 4Department of Ophthalmology, Showa University School of Medicine, Tokyo, JapanPurpose: Epithelial disorders after eye surgery can result in visual deterioration and patient discomfort. Such disorders may be caused by drug toxicity. In the present study, we evaluated the toxicity of ophthalmic solutions, with or without benzalkonium chloride (BAK) as the preservative, used for postoperative care.Methods: A range of commercially available antibiotic and anti-inflammatory ophthalmic solutions used postoperatively (ie, levofloxacin, moxifloxacin, gatifloxacin, norfloxacin, tosufloxacin, dibekacin, cefmenoxime, diclofenac, bromfenac, pranoprofen, betamethasone, and fluoromethorone) were assessed in three corneal cell lines and one conjunctival cell line. All antibiotic solutions were BAK free. Cell viability was determined with the 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay after cells had been exposed to the drugs for 48 h. The effects of preservatives on cell viability were also determined. Toxicity was compared using the cell viability score (CVS).Results: Based on results of the MTT assay and CVS, the order of cell viability after exposure to the antibiotic solutions was cefmenoxime &ge; tosufloxicin &ge; dibekacin &ge; levofloxacin &ge; norfloxacin = gatifloxacin = moxifloxacin. For the anti-inflammatory solutions, the order of cell viability was betamethasone &ge; betamethasone + fradiomycin &gt; preservative-free diclofenac &ge; preservative-free bromfenac &gt;&gt; 0.02% fluoromethorone &ge; 0.1% fluoromethorone = diclofenac + preservative = bromfenac + preservative = pranoprofen. The anti-inflammatory drugs were more toxic than the antibiotics. The toxicity of antibiotic drugs against ocular surface cells was dependent on the pharmaceutical components of the solution, whereas that of the anti-inflammatory drugs was dependent on both the pharmaceutical components and the preservatives.Conclusion: Postoperative drug-induced epitheliopathy may be caused primarily by anti-inflammatory drugs. CVS is useful in comparing the cytotoxicity of different drugs.Keywords: toxicity, preservative, cornea, eye Masahiko AyakiAtsuo IwasawaMitsutaka Sodaet alDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2010, Iss default, Pp 1019-1024 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Masahiko Ayaki Atsuo Iwasawa Mitsutaka Soda et al Cytotoxicity of five fluoroquinolone and two nonsteroidal anti-inflammatory benzalkonium chloride-free ophthalmic solutions in four corneoconjunctival cell lines
description	Masahiko Ayaki1, Atsuo Iwasawa2, Mitsutaka Soda3, Shigeo Yaguchi3, Ryohei Koide41Department of Ophthalmology, Saitama National Hospital, Saitama, Japan; 2Department of Clinical Pathology, Showa University Fujigaoka Hospital, Yokohama, Japan; 3Department of Ophthalmology, Showa University Fujigaoka Rehabilitation Hospital, Yokohama, Japan; 4Department of Ophthalmology, Showa University School of Medicine, Tokyo, JapanPurpose: Epithelial disorders after eye surgery can result in visual deterioration and patient discomfort. Such disorders may be caused by drug toxicity. In the present study, we evaluated the toxicity of ophthalmic solutions, with or without benzalkonium chloride (BAK) as the preservative, used for postoperative care.Methods: A range of commercially available antibiotic and anti-inflammatory ophthalmic solutions used postoperatively (ie, levofloxacin, moxifloxacin, gatifloxacin, norfloxacin, tosufloxacin, dibekacin, cefmenoxime, diclofenac, bromfenac, pranoprofen, betamethasone, and fluoromethorone) were assessed in three corneal cell lines and one conjunctival cell line. All antibiotic solutions were BAK free. Cell viability was determined with the 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay after cells had been exposed to the drugs for 48 h. The effects of preservatives on cell viability were also determined. Toxicity was compared using the cell viability score (CVS).Results: Based on results of the MTT assay and CVS, the order of cell viability after exposure to the antibiotic solutions was cefmenoxime &ge; tosufloxicin &ge; dibekacin &ge; levofloxacin &ge; norfloxacin = gatifloxacin = moxifloxacin. For the anti-inflammatory solutions, the order of cell viability was betamethasone &ge; betamethasone + fradiomycin &gt; preservative-free diclofenac &ge; preservative-free bromfenac &gt;&gt; 0.02% fluoromethorone &ge; 0.1% fluoromethorone = diclofenac + preservative = bromfenac + preservative = pranoprofen. The anti-inflammatory drugs were more toxic than the antibiotics. The toxicity of antibiotic drugs against ocular surface cells was dependent on the pharmaceutical components of the solution, whereas that of the anti-inflammatory drugs was dependent on both the pharmaceutical components and the preservatives.Conclusion: Postoperative drug-induced epitheliopathy may be caused primarily by anti-inflammatory drugs. CVS is useful in comparing the cytotoxicity of different drugs.Keywords: toxicity, preservative, cornea, eye
format	article
author	Masahiko Ayaki Atsuo Iwasawa Mitsutaka Soda et al
author_facet	Masahiko Ayaki Atsuo Iwasawa Mitsutaka Soda et al
author_sort	Masahiko Ayaki
title	Cytotoxicity of five fluoroquinolone and two nonsteroidal anti-inflammatory benzalkonium chloride-free ophthalmic solutions in four corneoconjunctival cell lines
title_short	Cytotoxicity of five fluoroquinolone and two nonsteroidal anti-inflammatory benzalkonium chloride-free ophthalmic solutions in four corneoconjunctival cell lines
title_full	Cytotoxicity of five fluoroquinolone and two nonsteroidal anti-inflammatory benzalkonium chloride-free ophthalmic solutions in four corneoconjunctival cell lines
title_fullStr	Cytotoxicity of five fluoroquinolone and two nonsteroidal anti-inflammatory benzalkonium chloride-free ophthalmic solutions in four corneoconjunctival cell lines
title_full_unstemmed	Cytotoxicity of five fluoroquinolone and two nonsteroidal anti-inflammatory benzalkonium chloride-free ophthalmic solutions in four corneoconjunctival cell lines
title_sort	cytotoxicity of five fluoroquinolone and two nonsteroidal anti-inflammatory benzalkonium chloride-free ophthalmic solutions in four corneoconjunctival cell lines
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/f3389f0b480b44af81d355d135db9b0b
work_keys_str_mv	AT masahikoayaki cytotoxicityoffivefluoroquinoloneandtwononsteroidalantiinflammatorybenzalkoniumchloridefreeophthalmicsolutionsinfourcorneoconjunctivalcelllines AT atsuoiwasawa cytotoxicityoffivefluoroquinoloneandtwononsteroidalantiinflammatorybenzalkoniumchloridefreeophthalmicsolutionsinfourcorneoconjunctivalcelllines AT mitsutakasoda cytotoxicityoffivefluoroquinoloneandtwononsteroidalantiinflammatorybenzalkoniumchloridefreeophthalmicsolutionsinfourcorneoconjunctivalcelllines AT etal cytotoxicityoffivefluoroquinoloneandtwononsteroidalantiinflammatorybenzalkoniumchloridefreeophthalmicsolutionsinfourcorneoconjunctivalcelllines
_version_	1718403532369952768

Cytotoxicity of five fluoroquinolone and two nonsteroidal anti-inflammatory benzalkonium chloride-free ophthalmic solutions in four corneoconjunctival cell lines

Ejemplares similares