Molecular imaging of myogenic stem/progenitor cells with [18F]-FHBG PET/CT system in SCID mice model of post-infarction heart

Abstract Preclinical and clinical studies have shown that stem cells can promote the regeneration of damaged tissues, but therapeutic protocols need better quality control to confirm the location and number of transplanted cells. This study describes in vivo imaging while assessing reporter gene exp...

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Autores principales:	Weronika Wargocka-Matuszewska, Katarzyna Fiedorowicz, Anna Rugowska, Karolina Bednarowicz, Agnieszka Zimna, Łukasz Cheda, Paulina Hamankiewicz, Krzysztof Kilian, Michał Fiedorowicz, Monika Drabik, Natalia Rozwadowska, Zbigniew Rogulski, Maciej Kurpisz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f33b98080133421aafe01e382c326a3b
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spelling	oai:doaj.org-article:f33b98080133421aafe01e382c326a3b2021-12-02T16:56:43ZMolecular imaging of myogenic stem/progenitor cells with [18F]-FHBG PET/CT system in SCID mice model of post-infarction heart10.1038/s41598-021-98861-52045-2322https://doaj.org/article/f33b98080133421aafe01e382c326a3b2021-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98861-5https://doaj.org/toc/2045-2322Abstract Preclinical and clinical studies have shown that stem cells can promote the regeneration of damaged tissues, but therapeutic protocols need better quality control to confirm the location and number of transplanted cells. This study describes in vivo imaging while assessing reporter gene expression by its binding to a radiolabelled molecule to the respective receptor expressed in target cells. Five mice underwent human skeletal muscle-derived stem/progenitor cell (huSkMDS/PC EF1-HSV-TK) intracardial transplantation after induction of myocardial infarction (MI). The metabolic parameters of control and post-infarction stem progenitor cell-implanted mice were monitored using 2-deoxy-18F-fluorodeoxyglucose ([18F]-FDG) before and after double promotor/reporter probe imaging with 9-(4-18F-fluoro-3-[hydroxymethyl]butyl)guanine ([18F]-FHBG) using positron emission tomography (PET) combined with computed tomography (CT). Standardized uptake values (SUVs) were then calculated based on set regions of interest (ROIs). Experimental animals were euthanized after magnetic resonance imaging (MRI). Molecular [18F]-FHBG imaging of myogenic stem/progenitor cells in control and post-infarction mice confirmed the survival and proliferation of transplanted cells, as shown by an increased or stable signal from the PET apparatus throughout the 5 weeks of monitoring. huSkMDS/PC EF1-HSV-TK transplantation improved cardiac metabolic ([18F]-FDG with PET) and haemodynamic (MRI) parameters. In vivo PET/CT and MRI revealed that the precise use of a promotor/reporter probe incorporated into stem/progenitor cells may improve non-invasive monitoring of targeted cellular therapy in the cardiovascular system.Weronika Wargocka-MatuszewskaKatarzyna FiedorowiczAnna RugowskaKarolina BednarowiczAgnieszka ZimnaŁukasz ChedaPaulina HamankiewiczKrzysztof KilianMichał FiedorowiczMonika DrabikNatalia RozwadowskaZbigniew RogulskiMaciej KurpiszNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Weronika Wargocka-Matuszewska Katarzyna Fiedorowicz Anna Rugowska Karolina Bednarowicz Agnieszka Zimna Łukasz Cheda Paulina Hamankiewicz Krzysztof Kilian Michał Fiedorowicz Monika Drabik Natalia Rozwadowska Zbigniew Rogulski Maciej Kurpisz Molecular imaging of myogenic stem/progenitor cells with [18F]-FHBG PET/CT system in SCID mice model of post-infarction heart
description	Abstract Preclinical and clinical studies have shown that stem cells can promote the regeneration of damaged tissues, but therapeutic protocols need better quality control to confirm the location and number of transplanted cells. This study describes in vivo imaging while assessing reporter gene expression by its binding to a radiolabelled molecule to the respective receptor expressed in target cells. Five mice underwent human skeletal muscle-derived stem/progenitor cell (huSkMDS/PC EF1-HSV-TK) intracardial transplantation after induction of myocardial infarction (MI). The metabolic parameters of control and post-infarction stem progenitor cell-implanted mice were monitored using 2-deoxy-18F-fluorodeoxyglucose ([18F]-FDG) before and after double promotor/reporter probe imaging with 9-(4-18F-fluoro-3-[hydroxymethyl]butyl)guanine ([18F]-FHBG) using positron emission tomography (PET) combined with computed tomography (CT). Standardized uptake values (SUVs) were then calculated based on set regions of interest (ROIs). Experimental animals were euthanized after magnetic resonance imaging (MRI). Molecular [18F]-FHBG imaging of myogenic stem/progenitor cells in control and post-infarction mice confirmed the survival and proliferation of transplanted cells, as shown by an increased or stable signal from the PET apparatus throughout the 5 weeks of monitoring. huSkMDS/PC EF1-HSV-TK transplantation improved cardiac metabolic ([18F]-FDG with PET) and haemodynamic (MRI) parameters. In vivo PET/CT and MRI revealed that the precise use of a promotor/reporter probe incorporated into stem/progenitor cells may improve non-invasive monitoring of targeted cellular therapy in the cardiovascular system.
format	article
author	Weronika Wargocka-Matuszewska Katarzyna Fiedorowicz Anna Rugowska Karolina Bednarowicz Agnieszka Zimna Łukasz Cheda Paulina Hamankiewicz Krzysztof Kilian Michał Fiedorowicz Monika Drabik Natalia Rozwadowska Zbigniew Rogulski Maciej Kurpisz
author_facet	Weronika Wargocka-Matuszewska Katarzyna Fiedorowicz Anna Rugowska Karolina Bednarowicz Agnieszka Zimna Łukasz Cheda Paulina Hamankiewicz Krzysztof Kilian Michał Fiedorowicz Monika Drabik Natalia Rozwadowska Zbigniew Rogulski Maciej Kurpisz
author_sort	Weronika Wargocka-Matuszewska
title	Molecular imaging of myogenic stem/progenitor cells with [18F]-FHBG PET/CT system in SCID mice model of post-infarction heart
title_short	Molecular imaging of myogenic stem/progenitor cells with [18F]-FHBG PET/CT system in SCID mice model of post-infarction heart
title_full	Molecular imaging of myogenic stem/progenitor cells with [18F]-FHBG PET/CT system in SCID mice model of post-infarction heart
title_fullStr	Molecular imaging of myogenic stem/progenitor cells with [18F]-FHBG PET/CT system in SCID mice model of post-infarction heart
title_full_unstemmed	Molecular imaging of myogenic stem/progenitor cells with [18F]-FHBG PET/CT system in SCID mice model of post-infarction heart
title_sort	molecular imaging of myogenic stem/progenitor cells with [18f]-fhbg pet/ct system in scid mice model of post-infarction heart
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f33b98080133421aafe01e382c326a3b
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Molecular imaging of myogenic stem/progenitor cells with [18F]-FHBG PET/CT system in SCID mice model of post-infarction heart

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