Effects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo

Currently, the mortality rate in Saudi Arabia’s ICUs is increasing due to the spread of Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria. This study was carried out to evaluate the ability of biologically synthesized zinc oxide nanoparticles (ZnO-NPs) using Aspergillus niger to overcome...

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Autores principales:	Elsayim Rasha, Manal M. Alkhulaifi, Monerah AlOthman, Ibrahim Khalid, Elnagar Doaa, Khatab Alaa, Manal A. Awad, Mohnad Abdalla
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	zinc oxide nanoparticle antimicrobial resistance biological synthesis carbapenem-resistant Klebsiella pneumoniae (KPC) Aspergillus niger wound recovery Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f33df42150f441c69467d43529c1e2dd
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spelling	oai:doaj.org-article:f33df42150f441c69467d43529c1e2dd2021-11-16T07:32:05ZEffects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo2235-298810.3389/fcimb.2021.748739https://doaj.org/article/f33df42150f441c69467d43529c1e2dd2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.748739/fullhttps://doaj.org/toc/2235-2988Currently, the mortality rate in Saudi Arabia’s ICUs is increasing due to the spread of Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria. This study was carried out to evaluate the ability of biologically synthesized zinc oxide nanoparticles (ZnO-NPs) using Aspergillus niger to overcome carbapenem-resistant K. pneumoniae (KPC) in vitro and in vivo. ZnO-NPs were synthesized via a biological method and characterized using UV–Vis spectroscopy, Zetasizer and zeta potential analyses, x-ray diffraction spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy (SEM), and energy-dispersive x-ray spectroscopy (EDX). In vitro sensitivity of KPC to ZnO-NPs was identified using the well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by a macro-dilution method. The morphological alteration of KPC cells after ZnO-NPs treatment was observed by SEM. The in vivo susceptibility of KPC cells to ZnO-NPs ointment was evaluated using wound healing in experimental rats. The chemical characterization findings showed the formation, stability, shape, and size of the synthesized nanoparticles. The MIC and MBC were 0.7 and 1.8 mg/ml, respectively. The in vivo results displayed reduced inflammation and wound re-epithelialization of KPC-infected rats. These findings demonstrated that ZnO-NPs have great potential to be developed as antibacterial agents.Elsayim RashaManal M. AlkhulaifiMonerah AlOthmanIbrahim KhalidElnagar DoaaKhatab AlaaManal A. AwadMohnad AbdallaFrontiers Media S.A.articlezinc oxide nanoparticleantimicrobial resistancebiological synthesiscarbapenem-resistant Klebsiella pneumoniae (KPC)Aspergillus nigerwound recoveryMicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	zinc oxide nanoparticle antimicrobial resistance biological synthesis carbapenem-resistant Klebsiella pneumoniae (KPC) Aspergillus niger wound recovery Microbiology QR1-502
spellingShingle	zinc oxide nanoparticle antimicrobial resistance biological synthesis carbapenem-resistant Klebsiella pneumoniae (KPC) Aspergillus niger wound recovery Microbiology QR1-502 Elsayim Rasha Manal M. Alkhulaifi Monerah AlOthman Ibrahim Khalid Elnagar Doaa Khatab Alaa Manal A. Awad Mohnad Abdalla Effects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo
description	Currently, the mortality rate in Saudi Arabia’s ICUs is increasing due to the spread of Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria. This study was carried out to evaluate the ability of biologically synthesized zinc oxide nanoparticles (ZnO-NPs) using Aspergillus niger to overcome carbapenem-resistant K. pneumoniae (KPC) in vitro and in vivo. ZnO-NPs were synthesized via a biological method and characterized using UV–Vis spectroscopy, Zetasizer and zeta potential analyses, x-ray diffraction spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy (SEM), and energy-dispersive x-ray spectroscopy (EDX). In vitro sensitivity of KPC to ZnO-NPs was identified using the well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by a macro-dilution method. The morphological alteration of KPC cells after ZnO-NPs treatment was observed by SEM. The in vivo susceptibility of KPC cells to ZnO-NPs ointment was evaluated using wound healing in experimental rats. The chemical characterization findings showed the formation, stability, shape, and size of the synthesized nanoparticles. The MIC and MBC were 0.7 and 1.8 mg/ml, respectively. The in vivo results displayed reduced inflammation and wound re-epithelialization of KPC-infected rats. These findings demonstrated that ZnO-NPs have great potential to be developed as antibacterial agents.
format	article
author	Elsayim Rasha Manal M. Alkhulaifi Monerah AlOthman Ibrahim Khalid Elnagar Doaa Khatab Alaa Manal A. Awad Mohnad Abdalla
author_facet	Elsayim Rasha Manal M. Alkhulaifi Monerah AlOthman Ibrahim Khalid Elnagar Doaa Khatab Alaa Manal A. Awad Mohnad Abdalla
author_sort	Elsayim Rasha
title	Effects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo
title_short	Effects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo
title_full	Effects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo
title_fullStr	Effects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo
title_full_unstemmed	Effects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo
title_sort	effects of zinc oxide nanoparticles synthesized using aspergillus niger on carbapenem-resistant klebsiella pneumonia in vitro and in vivo
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f33df42150f441c69467d43529c1e2dd
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Effects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo

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