An Innovative In Vitro Open-Angle Glaucoma Model (IVOM) Shows Changes Induced by Increased Ocular Pressure and Oxidative Stress
Primary Open-Angle Glaucoma (POAG) is a neurodegenerative disease, and its clinical outcomes lead to visual field constriction and blindness. POAG’s etiology is very complex and its pathogenesis is mainly explained through both mechanical and vascular theories. The trabecular meshwork (TM), the most...
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2021
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oai:doaj.org-article:f340dc1b1f00484c8e015951ea5f2fa02021-11-25T17:53:37ZAn Innovative In Vitro Open-Angle Glaucoma Model (IVOM) Shows Changes Induced by Increased Ocular Pressure and Oxidative Stress10.3390/ijms2222121291422-00671661-6596https://doaj.org/article/f340dc1b1f00484c8e015951ea5f2fa02021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12129https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Primary Open-Angle Glaucoma (POAG) is a neurodegenerative disease, and its clinical outcomes lead to visual field constriction and blindness. POAG’s etiology is very complex and its pathogenesis is mainly explained through both mechanical and vascular theories. The trabecular meshwork (TM), the most sensitive tissue of the eye anterior segment to oxidative stress (OS), is the main tissue involved in early-stage POAG, characterized by an increase in pressure. Preclinical assessments of neuroprotective drugs on animal models have not always shown correspondence with human clinical studies. In addition, intra-ocular pressure management after a glaucoma diagnosis does not always prevent blindness. Recently, we have been developing an innovative in vitro 3Dadvanced human trabecular cell model on a millifluidicplatform as a tool to improve glaucoma studies. Herein, we analyze the effects of prolonged increased pressure alone and, in association with OS, on such in vitro platform. Moreover, we verify whethersuch damaged TM triggers apoptosis on neuron-like cells. The preliminary results show that TM cells are less sensitive to pressure elevation than OS, and OS-damaging effects were worsened by the pressure increase. The stressed TM releases harmful signals, which increase apoptosis stimuli on neuron-like cells, suggesting its pivotal role in the glaucoma cascade.Stefania VernazzaSara TirendiMario PassalacquaFrancesco PiacenteSonia ScarfìFrancesco OddoneAnna Maria BassiMDPI AGarticle3D modelglaucomatrabecular meshworkbioreactorBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12129, p 12129 (2021) |
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DOAJ |
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3D model glaucoma trabecular meshwork bioreactor Biology (General) QH301-705.5 Chemistry QD1-999 |
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3D model glaucoma trabecular meshwork bioreactor Biology (General) QH301-705.5 Chemistry QD1-999 Stefania Vernazza Sara Tirendi Mario Passalacqua Francesco Piacente Sonia Scarfì Francesco Oddone Anna Maria Bassi An Innovative In Vitro Open-Angle Glaucoma Model (IVOM) Shows Changes Induced by Increased Ocular Pressure and Oxidative Stress |
| description |
Primary Open-Angle Glaucoma (POAG) is a neurodegenerative disease, and its clinical outcomes lead to visual field constriction and blindness. POAG’s etiology is very complex and its pathogenesis is mainly explained through both mechanical and vascular theories. The trabecular meshwork (TM), the most sensitive tissue of the eye anterior segment to oxidative stress (OS), is the main tissue involved in early-stage POAG, characterized by an increase in pressure. Preclinical assessments of neuroprotective drugs on animal models have not always shown correspondence with human clinical studies. In addition, intra-ocular pressure management after a glaucoma diagnosis does not always prevent blindness. Recently, we have been developing an innovative in vitro 3Dadvanced human trabecular cell model on a millifluidicplatform as a tool to improve glaucoma studies. Herein, we analyze the effects of prolonged increased pressure alone and, in association with OS, on such in vitro platform. Moreover, we verify whethersuch damaged TM triggers apoptosis on neuron-like cells. The preliminary results show that TM cells are less sensitive to pressure elevation than OS, and OS-damaging effects were worsened by the pressure increase. The stressed TM releases harmful signals, which increase apoptosis stimuli on neuron-like cells, suggesting its pivotal role in the glaucoma cascade. |
| format |
article |
| author |
Stefania Vernazza Sara Tirendi Mario Passalacqua Francesco Piacente Sonia Scarfì Francesco Oddone Anna Maria Bassi |
| author_facet |
Stefania Vernazza Sara Tirendi Mario Passalacqua Francesco Piacente Sonia Scarfì Francesco Oddone Anna Maria Bassi |
| author_sort |
Stefania Vernazza |
| title |
An Innovative In Vitro Open-Angle Glaucoma Model (IVOM) Shows Changes Induced by Increased Ocular Pressure and Oxidative Stress |
| title_short |
An Innovative In Vitro Open-Angle Glaucoma Model (IVOM) Shows Changes Induced by Increased Ocular Pressure and Oxidative Stress |
| title_full |
An Innovative In Vitro Open-Angle Glaucoma Model (IVOM) Shows Changes Induced by Increased Ocular Pressure and Oxidative Stress |
| title_fullStr |
An Innovative In Vitro Open-Angle Glaucoma Model (IVOM) Shows Changes Induced by Increased Ocular Pressure and Oxidative Stress |
| title_full_unstemmed |
An Innovative In Vitro Open-Angle Glaucoma Model (IVOM) Shows Changes Induced by Increased Ocular Pressure and Oxidative Stress |
| title_sort |
innovative in vitro open-angle glaucoma model (ivom) shows changes induced by increased ocular pressure and oxidative stress |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/f340dc1b1f00484c8e015951ea5f2fa0 |
| work_keys_str_mv |
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