Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells

Abstract The COS-7 (CV-1 in Origin with SV40 genes) cells are known as non-steroidogenic cells because they are derived from kidney cells and the kidney is defined as a non-steroidogenic organ. Therefore, COS-7 cells are used for transfection experiments to analyze the actions of functional molecule...

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Autores principales:	Mitsuki Nozaki, Shogo Haraguchi, Takuro Miyazaki, Daichi Shigeta, Noriko Kano, Xiao-Feng Lei, Joo-ri Kim-Kaneyama, Hiroyuki Minakata, Akira Miyazaki, Kazuyoshi Tsutsui
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f340fed7abf444b58fe307574375f127
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spelling	oai:doaj.org-article:f340fed7abf444b58fe307574375f1272021-12-02T15:08:07ZExpression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells10.1038/s41598-018-20226-22045-2322https://doaj.org/article/f340fed7abf444b58fe307574375f1272018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-20226-2https://doaj.org/toc/2045-2322Abstract The COS-7 (CV-1 in Origin with SV40 genes) cells are known as non-steroidogenic cells because they are derived from kidney cells and the kidney is defined as a non-steroidogenic organ. Therefore, COS-7 cells are used for transfection experiments to analyze the actions of functional molecules including steroids. However, a preliminary study suggested that COS-7 cells metabolize [3H]testosterone to [3H]androstenedione. These results suggest that COS-7 cells are able to metabolize steroids. Therefore, the present study investigated the expression of steroidogenic enzymes and the metabolism of steroids in COS-7 cells. RT-PCR analyses demonstrated the expressions of several kinds of steroidogenic enzymes, such as cytochrome P450 side-chain cleavage enzyme, 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase, cytochrome P450 7α-hydroxylase, cytochrome P450 17α-hydroxylase/17,20-lyase, 17β-hydroxysteroid dehydrogenase, 5α-reductase, cytochrome P450 21-hydroxylase, cytochrome P450 11β-hydroxylase, and cytochrome P450 aromatase in COS-7 cells. In addition, steroidogenic enzymes 3β-HSD, P4507α, 5α-reductase, P450c17, P450c21, P450c11β, and 17β-HSD actively metabolized various steroids in cultured COS-7 cells. Finally, we demonstrated that 17β-HSD activity toward androstenedione formation was greater than other steroidogenic enzyme activities. Our results provide new evidence that COS-7 cells express a series of steroidogenic enzyme mRNAs and actively metabolize a variety of steroids.Mitsuki NozakiShogo HaraguchiTakuro MiyazakiDaichi ShigetaNoriko KanoXiao-Feng LeiJoo-ri Kim-KaneyamaHiroyuki MinakataAkira MiyazakiKazuyoshi TsutsuiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mitsuki Nozaki Shogo Haraguchi Takuro Miyazaki Daichi Shigeta Noriko Kano Xiao-Feng Lei Joo-ri Kim-Kaneyama Hiroyuki Minakata Akira Miyazaki Kazuyoshi Tsutsui Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells
description	Abstract The COS-7 (CV-1 in Origin with SV40 genes) cells are known as non-steroidogenic cells because they are derived from kidney cells and the kidney is defined as a non-steroidogenic organ. Therefore, COS-7 cells are used for transfection experiments to analyze the actions of functional molecules including steroids. However, a preliminary study suggested that COS-7 cells metabolize [3H]testosterone to [3H]androstenedione. These results suggest that COS-7 cells are able to metabolize steroids. Therefore, the present study investigated the expression of steroidogenic enzymes and the metabolism of steroids in COS-7 cells. RT-PCR analyses demonstrated the expressions of several kinds of steroidogenic enzymes, such as cytochrome P450 side-chain cleavage enzyme, 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase, cytochrome P450 7α-hydroxylase, cytochrome P450 17α-hydroxylase/17,20-lyase, 17β-hydroxysteroid dehydrogenase, 5α-reductase, cytochrome P450 21-hydroxylase, cytochrome P450 11β-hydroxylase, and cytochrome P450 aromatase in COS-7 cells. In addition, steroidogenic enzymes 3β-HSD, P4507α, 5α-reductase, P450c17, P450c21, P450c11β, and 17β-HSD actively metabolized various steroids in cultured COS-7 cells. Finally, we demonstrated that 17β-HSD activity toward androstenedione formation was greater than other steroidogenic enzyme activities. Our results provide new evidence that COS-7 cells express a series of steroidogenic enzyme mRNAs and actively metabolize a variety of steroids.
format	article
author	Mitsuki Nozaki Shogo Haraguchi Takuro Miyazaki Daichi Shigeta Noriko Kano Xiao-Feng Lei Joo-ri Kim-Kaneyama Hiroyuki Minakata Akira Miyazaki Kazuyoshi Tsutsui
author_facet	Mitsuki Nozaki Shogo Haraguchi Takuro Miyazaki Daichi Shigeta Noriko Kano Xiao-Feng Lei Joo-ri Kim-Kaneyama Hiroyuki Minakata Akira Miyazaki Kazuyoshi Tsutsui
author_sort	Mitsuki Nozaki
title	Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells
title_short	Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells
title_full	Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells
title_fullStr	Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells
title_full_unstemmed	Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells
title_sort	expression of steroidogenic enzymes and metabolism of steroids in cos-7 cells known as non-steroidogenic cells
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/f340fed7abf444b58fe307574375f127
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Expression of steroidogenic enzymes and metabolism of steroids in COS-7 cells known as non-steroidogenic cells

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