Identification and Characterization of Nine Novel X-Chromosomal Short Tandem Repeats on Xp21.1, Xq21.31, and Xq23 Regions

The application of X-chromosomal short tandem repeats (X-STRs) has been recognized as a powerful tool in complex kinship testing. To support further development of X-STR analysis in forensic use, we identified nine novel X-STRs, which could be clustered into three linkage groups on Xp21.1, Xq21.31,...

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Autores principales:	Qinrui Yang, Jinglei Qian, Chengchen Shao, Yining Yao, Zhihan Zhou, Hongmei Xu, Qiqun Tang, Xiaoqin Qian, Jianhui Xie
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	X-chromosomal STRs multiplex PCR recombination linkage kinship testing Genetics QH426-470
Acceso en línea:	https://doaj.org/article/f3441016464d47538a897065d942622f
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spelling	oai:doaj.org-article:f3441016464d47538a897065d942622f2021-11-17T06:04:40ZIdentification and Characterization of Nine Novel X-Chromosomal Short Tandem Repeats on Xp21.1, Xq21.31, and Xq23 Regions1664-802110.3389/fgene.2021.784605https://doaj.org/article/f3441016464d47538a897065d942622f2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.784605/fullhttps://doaj.org/toc/1664-8021The application of X-chromosomal short tandem repeats (X-STRs) has been recognized as a powerful tool in complex kinship testing. To support further development of X-STR analysis in forensic use, we identified nine novel X-STRs, which could be clustered into three linkage groups on Xp21.1, Xq21.31, and Xq23. A multiplex PCR system was built based on the electrophoresis. A total of 198 unrelated Shanghai Han samples along with 168 samples from 43 families was collected to investigate the genetic polymorphism and forensic parameters of the nine loci. Allele numbers ranged from 5 to 12, and amplicon sizes ranged from 146 to 477 bp. The multiplex showed high values for the combined power of discrimination (0.99997977 in males and 0.99999999 in females) and combined mean exclusion chances (0.99997918 and 0.99997821 in trios, 0.99984939 in duos, and 0.99984200 in deficiency cases). The linkage between all pairs of loci was estimated via Kosambi mapping function and linkage disequilibrium test, and further investigated through the family study. The data from 43 families strongly demonstrated an independent transmission between LGs and a tight linkage among loci within the same LG. All these results support that the newly described X-STRs and the multiplex system are highly promising for further forensic use.Qinrui YangJinglei QianChengchen ShaoYining YaoZhihan ZhouHongmei XuQiqun TangXiaoqin QianJianhui XieFrontiers Media S.A.articleX-chromosomal STRsmultiplex PCRrecombinationlinkagekinship testingGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	X-chromosomal STRs multiplex PCR recombination linkage kinship testing Genetics QH426-470
spellingShingle	X-chromosomal STRs multiplex PCR recombination linkage kinship testing Genetics QH426-470 Qinrui Yang Jinglei Qian Chengchen Shao Yining Yao Zhihan Zhou Hongmei Xu Qiqun Tang Xiaoqin Qian Jianhui Xie Identification and Characterization of Nine Novel X-Chromosomal Short Tandem Repeats on Xp21.1, Xq21.31, and Xq23 Regions
description	The application of X-chromosomal short tandem repeats (X-STRs) has been recognized as a powerful tool in complex kinship testing. To support further development of X-STR analysis in forensic use, we identified nine novel X-STRs, which could be clustered into three linkage groups on Xp21.1, Xq21.31, and Xq23. A multiplex PCR system was built based on the electrophoresis. A total of 198 unrelated Shanghai Han samples along with 168 samples from 43 families was collected to investigate the genetic polymorphism and forensic parameters of the nine loci. Allele numbers ranged from 5 to 12, and amplicon sizes ranged from 146 to 477 bp. The multiplex showed high values for the combined power of discrimination (0.99997977 in males and 0.99999999 in females) and combined mean exclusion chances (0.99997918 and 0.99997821 in trios, 0.99984939 in duos, and 0.99984200 in deficiency cases). The linkage between all pairs of loci was estimated via Kosambi mapping function and linkage disequilibrium test, and further investigated through the family study. The data from 43 families strongly demonstrated an independent transmission between LGs and a tight linkage among loci within the same LG. All these results support that the newly described X-STRs and the multiplex system are highly promising for further forensic use.
format	article
author	Qinrui Yang Jinglei Qian Chengchen Shao Yining Yao Zhihan Zhou Hongmei Xu Qiqun Tang Xiaoqin Qian Jianhui Xie
author_facet	Qinrui Yang Jinglei Qian Chengchen Shao Yining Yao Zhihan Zhou Hongmei Xu Qiqun Tang Xiaoqin Qian Jianhui Xie
author_sort	Qinrui Yang
title	Identification and Characterization of Nine Novel X-Chromosomal Short Tandem Repeats on Xp21.1, Xq21.31, and Xq23 Regions
title_short	Identification and Characterization of Nine Novel X-Chromosomal Short Tandem Repeats on Xp21.1, Xq21.31, and Xq23 Regions
title_full	Identification and Characterization of Nine Novel X-Chromosomal Short Tandem Repeats on Xp21.1, Xq21.31, and Xq23 Regions
title_fullStr	Identification and Characterization of Nine Novel X-Chromosomal Short Tandem Repeats on Xp21.1, Xq21.31, and Xq23 Regions
title_full_unstemmed	Identification and Characterization of Nine Novel X-Chromosomal Short Tandem Repeats on Xp21.1, Xq21.31, and Xq23 Regions
title_sort	identification and characterization of nine novel x-chromosomal short tandem repeats on xp21.1, xq21.31, and xq23 regions
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f3441016464d47538a897065d942622f
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Identification and Characterization of Nine Novel X-Chromosomal Short Tandem Repeats on Xp21.1, Xq21.31, and Xq23 Regions

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