Cell differentiation of pluripotent tissue sheets immobilized on supported membranes displaying cadherin-11.
Investigating cohesive tissue sheets in controlled cultures still poses a challenge since the complex intercellular interactions are difficult to mimic in in vitro models. We used supported lipid membranes functionalized by the adhesive part of the extracellular domain of the cell adhesion molecule...
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2013
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oai:doaj.org-article:f34af24a65a94323b2eb4c0e800be3322021-11-18T07:57:59ZCell differentiation of pluripotent tissue sheets immobilized on supported membranes displaying cadherin-11.1932-620310.1371/journal.pone.0054749https://doaj.org/article/f34af24a65a94323b2eb4c0e800be3322013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23424619/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Investigating cohesive tissue sheets in controlled cultures still poses a challenge since the complex intercellular interactions are difficult to mimic in in vitro models. We used supported lipid membranes functionalized by the adhesive part of the extracellular domain of the cell adhesion molecule cadherin-11 for the immobilization of pluripotent tissue sheets, the animal cap isolated from Xenopus laevis blastula stage embryos. Cadherin-11 was bound via histidine tag to lipid membranes with chelator head groups. In the first step, quantitative functionalization of the membranes with cadherin-11 was confirmed by quartz crystal microbalance and high energy specular X-ray reflectivity. In the next step, animal cap tissue sheets induced to neural crest cell fate were cultured on the membranes functionalized with cadherin-11. The adhesion of cells within the cohesive tissue was significantly dependent on changes in lateral densities of cadherin-11. The formation of filopodia and lamellipodia in the cohesive tissue verified the viability and sustainability of the culture over several hours. The expression of the transcription factor slug in externally induced tissue demonstrated the applicability of lipid membranes displaying adhesive molecules for controlled differentiation of cohesive pluripotent tissue sheets.Alexander KörnerChristina DeichmannFernanda F RossettiAlmut KöhlerOleg V KonovalovDoris WedlichMotomu TanakaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e54749 (2013) |
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DOAJ |
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DOAJ |
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EN |
| topic |
Medicine R Science Q |
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Medicine R Science Q Alexander Körner Christina Deichmann Fernanda F Rossetti Almut Köhler Oleg V Konovalov Doris Wedlich Motomu Tanaka Cell differentiation of pluripotent tissue sheets immobilized on supported membranes displaying cadherin-11. |
| description |
Investigating cohesive tissue sheets in controlled cultures still poses a challenge since the complex intercellular interactions are difficult to mimic in in vitro models. We used supported lipid membranes functionalized by the adhesive part of the extracellular domain of the cell adhesion molecule cadherin-11 for the immobilization of pluripotent tissue sheets, the animal cap isolated from Xenopus laevis blastula stage embryos. Cadherin-11 was bound via histidine tag to lipid membranes with chelator head groups. In the first step, quantitative functionalization of the membranes with cadherin-11 was confirmed by quartz crystal microbalance and high energy specular X-ray reflectivity. In the next step, animal cap tissue sheets induced to neural crest cell fate were cultured on the membranes functionalized with cadherin-11. The adhesion of cells within the cohesive tissue was significantly dependent on changes in lateral densities of cadherin-11. The formation of filopodia and lamellipodia in the cohesive tissue verified the viability and sustainability of the culture over several hours. The expression of the transcription factor slug in externally induced tissue demonstrated the applicability of lipid membranes displaying adhesive molecules for controlled differentiation of cohesive pluripotent tissue sheets. |
| format |
article |
| author |
Alexander Körner Christina Deichmann Fernanda F Rossetti Almut Köhler Oleg V Konovalov Doris Wedlich Motomu Tanaka |
| author_facet |
Alexander Körner Christina Deichmann Fernanda F Rossetti Almut Köhler Oleg V Konovalov Doris Wedlich Motomu Tanaka |
| author_sort |
Alexander Körner |
| title |
Cell differentiation of pluripotent tissue sheets immobilized on supported membranes displaying cadherin-11. |
| title_short |
Cell differentiation of pluripotent tissue sheets immobilized on supported membranes displaying cadherin-11. |
| title_full |
Cell differentiation of pluripotent tissue sheets immobilized on supported membranes displaying cadherin-11. |
| title_fullStr |
Cell differentiation of pluripotent tissue sheets immobilized on supported membranes displaying cadherin-11. |
| title_full_unstemmed |
Cell differentiation of pluripotent tissue sheets immobilized on supported membranes displaying cadherin-11. |
| title_sort |
cell differentiation of pluripotent tissue sheets immobilized on supported membranes displaying cadherin-11. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/f34af24a65a94323b2eb4c0e800be332 |
| work_keys_str_mv |
AT alexanderkorner celldifferentiationofpluripotenttissuesheetsimmobilizedonsupportedmembranesdisplayingcadherin11 AT christinadeichmann celldifferentiationofpluripotenttissuesheetsimmobilizedonsupportedmembranesdisplayingcadherin11 AT fernandafrossetti celldifferentiationofpluripotenttissuesheetsimmobilizedonsupportedmembranesdisplayingcadherin11 AT almutkohler celldifferentiationofpluripotenttissuesheetsimmobilizedonsupportedmembranesdisplayingcadherin11 AT olegvkonovalov celldifferentiationofpluripotenttissuesheetsimmobilizedonsupportedmembranesdisplayingcadherin11 AT doriswedlich celldifferentiationofpluripotenttissuesheetsimmobilizedonsupportedmembranesdisplayingcadherin11 AT motomutanaka celldifferentiationofpluripotenttissuesheetsimmobilizedonsupportedmembranesdisplayingcadherin11 |
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