High-throughput format for the phenotyping of fungi on solid substrates

Abstract Filamentous fungi naturally grow on solid surfaces, yet most genetic and biochemical analyses are still performed in liquid cultures. Here, we report a multiplexing platform using high-throughput photometric continuous reading that allows parallel quantification of hyphal growth and reporte...

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Autores principales:	David Cánovas, Lena Studt, Ana T. Marcos, Joseph Strauss
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f34dc1b99622416aaa8d472f3a449d08
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spelling	oai:doaj.org-article:f34dc1b99622416aaa8d472f3a449d082021-12-02T11:52:15ZHigh-throughput format for the phenotyping of fungi on solid substrates10.1038/s41598-017-03598-92045-2322https://doaj.org/article/f34dc1b99622416aaa8d472f3a449d082017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03598-9https://doaj.org/toc/2045-2322Abstract Filamentous fungi naturally grow on solid surfaces, yet most genetic and biochemical analyses are still performed in liquid cultures. Here, we report a multiplexing platform using high-throughput photometric continuous reading that allows parallel quantification of hyphal growth and reporter gene expression directly on solid medium, thereby mimicking natural environmental conditions. Using this system, we have quantified fungal growth and expression of secondary metabolite GFP-based reporter genes in saprophytic Aspergillus and phytopathogenic Fusarium species in response to different nutrients, stress conditions and epigenetic modifiers. With this method, we provide not only novel insights into the characteristic of fungal growth but also into the metabolic and time-dependent regulation of secondary metabolite gene expression.David CánovasLena StudtAna T. MarcosJoseph StraussNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q David Cánovas Lena Studt Ana T. Marcos Joseph Strauss High-throughput format for the phenotyping of fungi on solid substrates
description	Abstract Filamentous fungi naturally grow on solid surfaces, yet most genetic and biochemical analyses are still performed in liquid cultures. Here, we report a multiplexing platform using high-throughput photometric continuous reading that allows parallel quantification of hyphal growth and reporter gene expression directly on solid medium, thereby mimicking natural environmental conditions. Using this system, we have quantified fungal growth and expression of secondary metabolite GFP-based reporter genes in saprophytic Aspergillus and phytopathogenic Fusarium species in response to different nutrients, stress conditions and epigenetic modifiers. With this method, we provide not only novel insights into the characteristic of fungal growth but also into the metabolic and time-dependent regulation of secondary metabolite gene expression.
format	article
author	David Cánovas Lena Studt Ana T. Marcos Joseph Strauss
author_facet	David Cánovas Lena Studt Ana T. Marcos Joseph Strauss
author_sort	David Cánovas
title	High-throughput format for the phenotyping of fungi on solid substrates
title_short	High-throughput format for the phenotyping of fungi on solid substrates
title_full	High-throughput format for the phenotyping of fungi on solid substrates
title_fullStr	High-throughput format for the phenotyping of fungi on solid substrates
title_full_unstemmed	High-throughput format for the phenotyping of fungi on solid substrates
title_sort	high-throughput format for the phenotyping of fungi on solid substrates
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f34dc1b99622416aaa8d472f3a449d08
work_keys_str_mv	AT davidcanovas highthroughputformatforthephenotypingoffungionsolidsubstrates AT lenastudt highthroughputformatforthephenotypingoffungionsolidsubstrates AT anatmarcos highthroughputformatforthephenotypingoffungionsolidsubstrates AT josephstrauss highthroughputformatforthephenotypingoffungionsolidsubstrates
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High-throughput format for the phenotyping of fungi on solid substrates

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