Linalool effect on mitochondrial bioenergetics rat liver

Background: The linalool (2,6-dimethyl-2 ,7-octadiene-6-ol) is a monoterpene present in more tan 200 types of plants, and it is the major component of several essential oils. The linalool has a wide range of potential pharmacological properties, such as antiseptic, anxiolytic, anti-inflammatory, an...

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Autores principales:	Yenny BUENO-DUARTE, Stelia Carolina MÉNDEZ-SÁNCHEZ
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2015
Materias:	Linalool mitochondria enzymes oxygen consumption Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/f35671fe57e14422903e579f45c38cdc
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spelling	oai:doaj.org-article:f35671fe57e14422903e579f45c38cdc2021-11-19T04:10:30ZLinalool effect on mitochondrial bioenergetics rat liver0121-40042145-2660https://doaj.org/article/f35671fe57e14422903e579f45c38cdc2015-07-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/19740https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660 Background: The linalool (2,6-dimethyl-2 ,7-octadiene-6-ol) is a monoterpene present in more tan 200 types of plants, and it is the major component of several essential oils. The linalool has a wide range of potential pharmacological properties, such as antiseptic, anxiolytic, anti-inflammatory, antifungal, antimicrobial and proapoptotic in different tumor cell lines. However, its primary use is associated as an odorant molecule or fragrance in topical products, in cosmetics products and fragrance industry. Objective: In this study the effect of linalool on the bioenergetics of isolated rat liver mitochondria was evaluated. Methods: Linalool in concentrations between (5-100μg/mL) was evaluated in isolated mitochondria of male albino Wistar rats, according to the method described by Voss et al (1961). Initially, the oxygen consumption by intact mitochondria was determined by polarographic method using an oxygraph. Subsequently, the effect of the compound on mitochondrial respiratory chain enzymes was evaluated, by using polarographic and spectrophotometric methods. Results: The results obtained indicated that linalool, at concentrations of 5, 10, 50 and 100μg/mL, affects the rate of oxygen consumption in state 3 and 4 of mitochondria energized with glutamate-malate and succinate. However, the inhibitory effect was greater when using glutamate-malate as substrate. For its part, the analysis of the enzyme activities of the mitochondrial respiratory chain showed that linalool (50μg/mL) inhibits complex I, III, and ATPase activity. Conclusions: linalool inhibits complex I, complex III and ATPase, this inhibition could increase the generation of reactive oxygen species. Yenny BUENO-DUARTEStelia Carolina MÉNDEZ-SÁNCHEZUniversidad de AntioquiaarticleLinaloolmitochondriaenzymesoxygen consumptionFood processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 22, Iss 1 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Linalool mitochondria enzymes oxygen consumption Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	Linalool mitochondria enzymes oxygen consumption Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Yenny BUENO-DUARTE Stelia Carolina MÉNDEZ-SÁNCHEZ Linalool effect on mitochondrial bioenergetics rat liver
description	Background: The linalool (2,6-dimethyl-2 ,7-octadiene-6-ol) is a monoterpene present in more tan 200 types of plants, and it is the major component of several essential oils. The linalool has a wide range of potential pharmacological properties, such as antiseptic, anxiolytic, anti-inflammatory, antifungal, antimicrobial and proapoptotic in different tumor cell lines. However, its primary use is associated as an odorant molecule or fragrance in topical products, in cosmetics products and fragrance industry. Objective: In this study the effect of linalool on the bioenergetics of isolated rat liver mitochondria was evaluated. Methods: Linalool in concentrations between (5-100μg/mL) was evaluated in isolated mitochondria of male albino Wistar rats, according to the method described by Voss et al (1961). Initially, the oxygen consumption by intact mitochondria was determined by polarographic method using an oxygraph. Subsequently, the effect of the compound on mitochondrial respiratory chain enzymes was evaluated, by using polarographic and spectrophotometric methods. Results: The results obtained indicated that linalool, at concentrations of 5, 10, 50 and 100μg/mL, affects the rate of oxygen consumption in state 3 and 4 of mitochondria energized with glutamate-malate and succinate. However, the inhibitory effect was greater when using glutamate-malate as substrate. For its part, the analysis of the enzyme activities of the mitochondrial respiratory chain showed that linalool (50μg/mL) inhibits complex I, III, and ATPase activity. Conclusions: linalool inhibits complex I, complex III and ATPase, this inhibition could increase the generation of reactive oxygen species.
format	article
author	Yenny BUENO-DUARTE Stelia Carolina MÉNDEZ-SÁNCHEZ
author_facet	Yenny BUENO-DUARTE Stelia Carolina MÉNDEZ-SÁNCHEZ
author_sort	Yenny BUENO-DUARTE
title	Linalool effect on mitochondrial bioenergetics rat liver
title_short	Linalool effect on mitochondrial bioenergetics rat liver
title_full	Linalool effect on mitochondrial bioenergetics rat liver
title_fullStr	Linalool effect on mitochondrial bioenergetics rat liver
title_full_unstemmed	Linalool effect on mitochondrial bioenergetics rat liver
title_sort	linalool effect on mitochondrial bioenergetics rat liver
publisher	Universidad de Antioquia
publishDate	2015
url	https://doaj.org/article/f35671fe57e14422903e579f45c38cdc
work_keys_str_mv	AT yennybuenoduarte linalooleffectonmitochondrialbioenergeticsratliver AT steliacarolinamendezsanchez linalooleffectonmitochondrialbioenergeticsratliver
_version_	1718420528340926464

Linalool effect on mitochondrial bioenergetics rat liver

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