Improved detection of prostate cancer using a magneto-nanosensor assay for serum circulating autoantibodies.

<h4>Purpose</h4>To develop a magneto-nanosensor (MNS) based multiplex assay to measure protein and autoantibody biomarkers from human serum for prostate cancer (CaP) diagnosis.<h4>Materials and methods</h4>A 4-panel MNS autoantibody assay and a MNS protein assay were develope...

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Autores principales:	Lingyun Xu, Jung-Rok Lee, Shiying Hao, Xuefeng Bruce Ling, James D Brooks, Shan X Wang, Sanjiv Sam Gambhir
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f35b50268bee436a98c0ece7c039aa61
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spelling	oai:doaj.org-article:f35b50268bee436a98c0ece7c039aa612021-12-02T20:15:52ZImproved detection of prostate cancer using a magneto-nanosensor assay for serum circulating autoantibodies.1932-620310.1371/journal.pone.0221051https://doaj.org/article/f35b50268bee436a98c0ece7c039aa612019-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0221051https://doaj.org/toc/1932-6203<h4>Purpose</h4>To develop a magneto-nanosensor (MNS) based multiplex assay to measure protein and autoantibody biomarkers from human serum for prostate cancer (CaP) diagnosis.<h4>Materials and methods</h4>A 4-panel MNS autoantibody assay and a MNS protein assay were developed and optimized in our labs. Using these assays, serum concentration of six biomarkers including prostate-specific antigen (PSA) protein, free/total PSA ratio, as well as four autoantibodies against Parkinson disease 7 (PARK7), TAR DNA-binding protein 43 (TARDBP), Talin 1 (TLN1), and Caldesmon 1 (CALD1) and were analyzed. Human serum samples from 99 patients (50 with non-cancer and 49 with clinically localized CaP) were evaluated.<h4>Results</h4>The MNS assay showed excellent performance characteristics and no cross-reactivity. All autoantibody assays showed a statistically significant difference between CaP and non-cancer samples except for PARK7. The most significant difference was the combination of the four autoantibodies as a panel in addition to the free/total PSA ratio. This combination had the highest area under the curve (AUC)- 0.916 in ROC analysis.<h4>Conclusions</h4>Our results suggest that this autoantibody panel along with PSA and free PSA have potential to segregate patients without cancer from those with prostate cancer with higher sensitivity and specificity than PSA alone.Lingyun XuJung-Rok LeeShiying HaoXuefeng Bruce LingJames D BrooksShan X WangSanjiv Sam GambhirPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 14, Iss 8, p e0221051 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lingyun Xu Jung-Rok Lee Shiying Hao Xuefeng Bruce Ling James D Brooks Shan X Wang Sanjiv Sam Gambhir Improved detection of prostate cancer using a magneto-nanosensor assay for serum circulating autoantibodies.
description	<h4>Purpose</h4>To develop a magneto-nanosensor (MNS) based multiplex assay to measure protein and autoantibody biomarkers from human serum for prostate cancer (CaP) diagnosis.<h4>Materials and methods</h4>A 4-panel MNS autoantibody assay and a MNS protein assay were developed and optimized in our labs. Using these assays, serum concentration of six biomarkers including prostate-specific antigen (PSA) protein, free/total PSA ratio, as well as four autoantibodies against Parkinson disease 7 (PARK7), TAR DNA-binding protein 43 (TARDBP), Talin 1 (TLN1), and Caldesmon 1 (CALD1) and were analyzed. Human serum samples from 99 patients (50 with non-cancer and 49 with clinically localized CaP) were evaluated.<h4>Results</h4>The MNS assay showed excellent performance characteristics and no cross-reactivity. All autoantibody assays showed a statistically significant difference between CaP and non-cancer samples except for PARK7. The most significant difference was the combination of the four autoantibodies as a panel in addition to the free/total PSA ratio. This combination had the highest area under the curve (AUC)- 0.916 in ROC analysis.<h4>Conclusions</h4>Our results suggest that this autoantibody panel along with PSA and free PSA have potential to segregate patients without cancer from those with prostate cancer with higher sensitivity and specificity than PSA alone.
format	article
author	Lingyun Xu Jung-Rok Lee Shiying Hao Xuefeng Bruce Ling James D Brooks Shan X Wang Sanjiv Sam Gambhir
author_facet	Lingyun Xu Jung-Rok Lee Shiying Hao Xuefeng Bruce Ling James D Brooks Shan X Wang Sanjiv Sam Gambhir
author_sort	Lingyun Xu
title	Improved detection of prostate cancer using a magneto-nanosensor assay for serum circulating autoantibodies.
title_short	Improved detection of prostate cancer using a magneto-nanosensor assay for serum circulating autoantibodies.
title_full	Improved detection of prostate cancer using a magneto-nanosensor assay for serum circulating autoantibodies.
title_fullStr	Improved detection of prostate cancer using a magneto-nanosensor assay for serum circulating autoantibodies.
title_full_unstemmed	Improved detection of prostate cancer using a magneto-nanosensor assay for serum circulating autoantibodies.
title_sort	improved detection of prostate cancer using a magneto-nanosensor assay for serum circulating autoantibodies.
publisher	Public Library of Science (PLoS)
publishDate	2019
url	https://doaj.org/article/f35b50268bee436a98c0ece7c039aa61
work_keys_str_mv	AT lingyunxu improveddetectionofprostatecancerusingamagnetonanosensorassayforserumcirculatingautoantibodies AT jungroklee improveddetectionofprostatecancerusingamagnetonanosensorassayforserumcirculatingautoantibodies AT shiyinghao improveddetectionofprostatecancerusingamagnetonanosensorassayforserumcirculatingautoantibodies AT xuefengbruceling improveddetectionofprostatecancerusingamagnetonanosensorassayforserumcirculatingautoantibodies AT jamesdbrooks improveddetectionofprostatecancerusingamagnetonanosensorassayforserumcirculatingautoantibodies AT shanxwang improveddetectionofprostatecancerusingamagnetonanosensorassayforserumcirculatingautoantibodies AT sanjivsamgambhir improveddetectionofprostatecancerusingamagnetonanosensorassayforserumcirculatingautoantibodies
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Improved detection of prostate cancer using a magneto-nanosensor assay for serum circulating autoantibodies.

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