Efficient Control of Zika Virus Infection Induced by a Non-Replicating Adenovector Encoding Zika Virus NS1/NS2 Antigens Fused to the MHC Class II-Associated Invariant Chain

It is generally believed that a successful Zika virus (ZIKV) vaccine should induce neutralizing antibodies against the ZIKV envelope (E) protein to efficiently halt viral infection. However, E-specific neutralizing antibodies have been implicated in a phenomenon called antibody-dependent enhancement...

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Autores principales:	Loulieta Nazerai, Søren Buus, Anette Stryhn, Allan Randrup Thomsen, Jan Pravsgaard Christensen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Zika virus vaccine NS1/NS2 CD8 T cell response MHC-II invariant chain Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f35c58cfd4ae4020b4a0433eafa35e0c
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spelling	oai:doaj.org-article:f35c58cfd4ae4020b4a0433eafa35e0c2021-11-25T19:13:24ZEfficient Control of Zika Virus Infection Induced by a Non-Replicating Adenovector Encoding Zika Virus NS1/NS2 Antigens Fused to the MHC Class II-Associated Invariant Chain10.3390/v131122151999-4915https://doaj.org/article/f35c58cfd4ae4020b4a0433eafa35e0c2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2215https://doaj.org/toc/1999-4915It is generally believed that a successful Zika virus (ZIKV) vaccine should induce neutralizing antibodies against the ZIKV envelope (E) protein to efficiently halt viral infection. However, E-specific neutralizing antibodies have been implicated in a phenomenon called antibody-dependent enhancement, which represents an ongoing concern in the flavivirus-vaccinology field. In this report, we investigated the vaccination potential of replication-deficient adenoviral vectors encoding the ZIKV non-structural proteins 1 and 2 (NS1/NS2) and employed the strategy of linking the antigens to the MHC-II associated invariant chain (li) to improve immunogenicity and by inference, the level of protection. We demonstrated that li-linkage enhanced the production of anti-NS1 antibodies and induced an accelerated and prolonged polyfunctional CD8 T cell response in mice, which ultimately resulted in a high degree of protection against ZIKV infection of the CNS.Loulieta NazeraiSøren BuusAnette StryhnAllan Randrup ThomsenJan Pravsgaard ChristensenMDPI AGarticleZika virusvaccineNS1/NS2CD8 T cell responseMHC-II invariant chainMicrobiologyQR1-502ENViruses, Vol 13, Iss 2215, p 2215 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Zika virus vaccine NS1/NS2 CD8 T cell response MHC-II invariant chain Microbiology QR1-502
spellingShingle	Zika virus vaccine NS1/NS2 CD8 T cell response MHC-II invariant chain Microbiology QR1-502 Loulieta Nazerai Søren Buus Anette Stryhn Allan Randrup Thomsen Jan Pravsgaard Christensen Efficient Control of Zika Virus Infection Induced by a Non-Replicating Adenovector Encoding Zika Virus NS1/NS2 Antigens Fused to the MHC Class II-Associated Invariant Chain
description	It is generally believed that a successful Zika virus (ZIKV) vaccine should induce neutralizing antibodies against the ZIKV envelope (E) protein to efficiently halt viral infection. However, E-specific neutralizing antibodies have been implicated in a phenomenon called antibody-dependent enhancement, which represents an ongoing concern in the flavivirus-vaccinology field. In this report, we investigated the vaccination potential of replication-deficient adenoviral vectors encoding the ZIKV non-structural proteins 1 and 2 (NS1/NS2) and employed the strategy of linking the antigens to the MHC-II associated invariant chain (li) to improve immunogenicity and by inference, the level of protection. We demonstrated that li-linkage enhanced the production of anti-NS1 antibodies and induced an accelerated and prolonged polyfunctional CD8 T cell response in mice, which ultimately resulted in a high degree of protection against ZIKV infection of the CNS.
format	article
author	Loulieta Nazerai Søren Buus Anette Stryhn Allan Randrup Thomsen Jan Pravsgaard Christensen
author_facet	Loulieta Nazerai Søren Buus Anette Stryhn Allan Randrup Thomsen Jan Pravsgaard Christensen
author_sort	Loulieta Nazerai
title	Efficient Control of Zika Virus Infection Induced by a Non-Replicating Adenovector Encoding Zika Virus NS1/NS2 Antigens Fused to the MHC Class II-Associated Invariant Chain
title_short	Efficient Control of Zika Virus Infection Induced by a Non-Replicating Adenovector Encoding Zika Virus NS1/NS2 Antigens Fused to the MHC Class II-Associated Invariant Chain
title_full	Efficient Control of Zika Virus Infection Induced by a Non-Replicating Adenovector Encoding Zika Virus NS1/NS2 Antigens Fused to the MHC Class II-Associated Invariant Chain
title_fullStr	Efficient Control of Zika Virus Infection Induced by a Non-Replicating Adenovector Encoding Zika Virus NS1/NS2 Antigens Fused to the MHC Class II-Associated Invariant Chain
title_full_unstemmed	Efficient Control of Zika Virus Infection Induced by a Non-Replicating Adenovector Encoding Zika Virus NS1/NS2 Antigens Fused to the MHC Class II-Associated Invariant Chain
title_sort	efficient control of zika virus infection induced by a non-replicating adenovector encoding zika virus ns1/ns2 antigens fused to the mhc class ii-associated invariant chain
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f35c58cfd4ae4020b4a0433eafa35e0c
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Efficient Control of Zika Virus Infection Induced by a Non-Replicating Adenovector Encoding Zika Virus NS1/NS2 Antigens Fused to the MHC Class II-Associated Invariant Chain

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