The role of the st313-td gene in virulence of Salmonella Typhimurium ST313.

Multidrug-resistant Salmonella enterica serovar Typhimurium ST313 has emerged in sub-Saharan Africa causing severe infections in humans. Therefore, it has been speculated that this specific sequence type, ST313, carries factors associated with increased pathogenicity. We assessed the role in virulen...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ana Herrero-Fresno, Inke Wallrodt, Pimlapas Leekitcharoenphon, John Elmerdahl Olsen, Frank M Aarestrup, Rene S Hendriksen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f360241f46d4444faaf0c9f464f48bcf
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f360241f46d4444faaf0c9f464f48bcf
record_format	dspace
spelling	oai:doaj.org-article:f360241f46d4444faaf0c9f464f48bcf2021-11-18T08:38:57ZThe role of the st313-td gene in virulence of Salmonella Typhimurium ST313.1932-620310.1371/journal.pone.0084566https://doaj.org/article/f360241f46d4444faaf0c9f464f48bcf2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24404174/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Multidrug-resistant Salmonella enterica serovar Typhimurium ST313 has emerged in sub-Saharan Africa causing severe infections in humans. Therefore, it has been speculated that this specific sequence type, ST313, carries factors associated with increased pathogenicity. We assessed the role in virulence of a gene with a yet unknown function, st313-td, detected in ST313 through comparative genomics. Additionally, the structure of the genomic island ST313-GI, harbouring the gene was determined. The gene st313-td was cloned into wild type S. Typhimurium 4/74 (4/74-C) as well as knocked out in S. Typhimurium ST313 02-03/002 (Δst313-td) followed by complementation (02-03/002-C). Δst313-td was less virulent in mice following i.p. challenge than the wild type and this phenotype could be partly complemented in trans, indicating that st313-td plays a role during systemic infection. The gene st313-td was shown not to affect invasion of cultured epithelial cells, while the absence of the gene significantly affects uptake and intracellular survival within macrophages. The gene st313-td was proven to be strongly associated to invasiveness, harboured by 92.5% of S. Typhimurium blood isolates (n = 82) and 100% of S. Dublin strains (n = 50) analysed. On the contrary, S. Typhimurium isolates of animal and food origin (n = 82) did not carry st313-td. Six human, non-blood isolates of S. Typhimurium from Belarus, China and Nepal harboured the gene and belonged to sequence types ST398 and ST19. Our data showed a global presence of the st313-td gene and in other sequence types than ST313. The gene st313-td was shown to be expressed during logarithmic phase of growth in 14 selected Salmonella strains carrying the gene. This study reveals that st313-td plays a role in S. Typhimurium ST313 pathogenesis and adds another chapter to understanding of the virulence of S. Typhimurium and in particular of the emerging sequence type ST313.Ana Herrero-FresnoInke WallrodtPimlapas LeekitcharoenphonJohn Elmerdahl OlsenFrank M AarestrupRene S HendriksenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e84566 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ana Herrero-Fresno Inke Wallrodt Pimlapas Leekitcharoenphon John Elmerdahl Olsen Frank M Aarestrup Rene S Hendriksen The role of the st313-td gene in virulence of Salmonella Typhimurium ST313.
description	Multidrug-resistant Salmonella enterica serovar Typhimurium ST313 has emerged in sub-Saharan Africa causing severe infections in humans. Therefore, it has been speculated that this specific sequence type, ST313, carries factors associated with increased pathogenicity. We assessed the role in virulence of a gene with a yet unknown function, st313-td, detected in ST313 through comparative genomics. Additionally, the structure of the genomic island ST313-GI, harbouring the gene was determined. The gene st313-td was cloned into wild type S. Typhimurium 4/74 (4/74-C) as well as knocked out in S. Typhimurium ST313 02-03/002 (Δst313-td) followed by complementation (02-03/002-C). Δst313-td was less virulent in mice following i.p. challenge than the wild type and this phenotype could be partly complemented in trans, indicating that st313-td plays a role during systemic infection. The gene st313-td was shown not to affect invasion of cultured epithelial cells, while the absence of the gene significantly affects uptake and intracellular survival within macrophages. The gene st313-td was proven to be strongly associated to invasiveness, harboured by 92.5% of S. Typhimurium blood isolates (n = 82) and 100% of S. Dublin strains (n = 50) analysed. On the contrary, S. Typhimurium isolates of animal and food origin (n = 82) did not carry st313-td. Six human, non-blood isolates of S. Typhimurium from Belarus, China and Nepal harboured the gene and belonged to sequence types ST398 and ST19. Our data showed a global presence of the st313-td gene and in other sequence types than ST313. The gene st313-td was shown to be expressed during logarithmic phase of growth in 14 selected Salmonella strains carrying the gene. This study reveals that st313-td plays a role in S. Typhimurium ST313 pathogenesis and adds another chapter to understanding of the virulence of S. Typhimurium and in particular of the emerging sequence type ST313.
format	article
author	Ana Herrero-Fresno Inke Wallrodt Pimlapas Leekitcharoenphon John Elmerdahl Olsen Frank M Aarestrup Rene S Hendriksen
author_facet	Ana Herrero-Fresno Inke Wallrodt Pimlapas Leekitcharoenphon John Elmerdahl Olsen Frank M Aarestrup Rene S Hendriksen
author_sort	Ana Herrero-Fresno
title	The role of the st313-td gene in virulence of Salmonella Typhimurium ST313.
title_short	The role of the st313-td gene in virulence of Salmonella Typhimurium ST313.
title_full	The role of the st313-td gene in virulence of Salmonella Typhimurium ST313.
title_fullStr	The role of the st313-td gene in virulence of Salmonella Typhimurium ST313.
title_full_unstemmed	The role of the st313-td gene in virulence of Salmonella Typhimurium ST313.
title_sort	role of the st313-td gene in virulence of salmonella typhimurium st313.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/f360241f46d4444faaf0c9f464f48bcf
work_keys_str_mv	AT anaherrerofresno theroleofthest313tdgeneinvirulenceofsalmonellatyphimuriumst313 AT inkewallrodt theroleofthest313tdgeneinvirulenceofsalmonellatyphimuriumst313 AT pimlapasleekitcharoenphon theroleofthest313tdgeneinvirulenceofsalmonellatyphimuriumst313 AT johnelmerdahlolsen theroleofthest313tdgeneinvirulenceofsalmonellatyphimuriumst313 AT frankmaarestrup theroleofthest313tdgeneinvirulenceofsalmonellatyphimuriumst313 AT reneshendriksen theroleofthest313tdgeneinvirulenceofsalmonellatyphimuriumst313 AT anaherrerofresno roleofthest313tdgeneinvirulenceofsalmonellatyphimuriumst313 AT inkewallrodt roleofthest313tdgeneinvirulenceofsalmonellatyphimuriumst313 AT pimlapasleekitcharoenphon roleofthest313tdgeneinvirulenceofsalmonellatyphimuriumst313 AT johnelmerdahlolsen roleofthest313tdgeneinvirulenceofsalmonellatyphimuriumst313 AT frankmaarestrup roleofthest313tdgeneinvirulenceofsalmonellatyphimuriumst313 AT reneshendriksen roleofthest313tdgeneinvirulenceofsalmonellatyphimuriumst313
_version_	1718421537263976448

The role of the st313-td gene in virulence of Salmonella Typhimurium ST313.

Ejemplares similares