Sequential Membrane Rupture and Vesiculation during Plasmodium berghei Gametocyte Egress from the Red Blood Cell

Abstract Malaria parasites alternate between intracellular and extracellular stages and successful egress from the host cell is crucial for continuation of the life cycle. We investigated egress of Plasmodium berghei gametocytes, an essential process taking place within a few minutes after uptake of...

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Autores principales:	Maria Andreadaki, Eric Hanssen, Elena Deligianni, Cyrille Claudet, Kai Wengelnik, Vanessa Mollard, Geoffrey I. McFadden, Manouk Abkarian, Catherine Braun-Breton, Inga Siden-Kiamos
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f364a0dae290472993a2c088f8fb673d
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spelling	oai:doaj.org-article:f364a0dae290472993a2c088f8fb673d2021-12-02T15:08:41ZSequential Membrane Rupture and Vesiculation during Plasmodium berghei Gametocyte Egress from the Red Blood Cell10.1038/s41598-018-21801-32045-2322https://doaj.org/article/f364a0dae290472993a2c088f8fb673d2018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21801-3https://doaj.org/toc/2045-2322Abstract Malaria parasites alternate between intracellular and extracellular stages and successful egress from the host cell is crucial for continuation of the life cycle. We investigated egress of Plasmodium berghei gametocytes, an essential process taking place within a few minutes after uptake of a blood meal by the mosquito. Egress entails the rupture of two membranes surrounding the parasite: the parasitophorous vacuole membrane (PVM), and the red blood cell membrane (RBCM). High-speed video microscopy of 56 events revealed that egress in both genders comprises four well-defined phases, although each event is slightly different. The first phase is swelling of the host cell, followed by rupture and immediate vesiculation of the PVM. These vesicles are extruded through a single stabilized pore of the RBCM, and the latter is subsequently vesiculated releasing the free gametes. The time from PVM vesiculation to completion of egress varies between events. These observations were supported by immunofluorescence microscopy using antibodies against proteins of the RBCM and PVM. The combined results reveal dynamic re-organization of the membranes and the cortical cytoskeleton of the erythrocyte during egress.Maria AndreadakiEric HanssenElena DeligianniCyrille ClaudetKai WengelnikVanessa MollardGeoffrey I. McFaddenManouk AbkarianCatherine Braun-BretonInga Siden-KiamosNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria Andreadaki Eric Hanssen Elena Deligianni Cyrille Claudet Kai Wengelnik Vanessa Mollard Geoffrey I. McFadden Manouk Abkarian Catherine Braun-Breton Inga Siden-Kiamos Sequential Membrane Rupture and Vesiculation during Plasmodium berghei Gametocyte Egress from the Red Blood Cell
description	Abstract Malaria parasites alternate between intracellular and extracellular stages and successful egress from the host cell is crucial for continuation of the life cycle. We investigated egress of Plasmodium berghei gametocytes, an essential process taking place within a few minutes after uptake of a blood meal by the mosquito. Egress entails the rupture of two membranes surrounding the parasite: the parasitophorous vacuole membrane (PVM), and the red blood cell membrane (RBCM). High-speed video microscopy of 56 events revealed that egress in both genders comprises four well-defined phases, although each event is slightly different. The first phase is swelling of the host cell, followed by rupture and immediate vesiculation of the PVM. These vesicles are extruded through a single stabilized pore of the RBCM, and the latter is subsequently vesiculated releasing the free gametes. The time from PVM vesiculation to completion of egress varies between events. These observations were supported by immunofluorescence microscopy using antibodies against proteins of the RBCM and PVM. The combined results reveal dynamic re-organization of the membranes and the cortical cytoskeleton of the erythrocyte during egress.
format	article
author	Maria Andreadaki Eric Hanssen Elena Deligianni Cyrille Claudet Kai Wengelnik Vanessa Mollard Geoffrey I. McFadden Manouk Abkarian Catherine Braun-Breton Inga Siden-Kiamos
author_facet	Maria Andreadaki Eric Hanssen Elena Deligianni Cyrille Claudet Kai Wengelnik Vanessa Mollard Geoffrey I. McFadden Manouk Abkarian Catherine Braun-Breton Inga Siden-Kiamos
author_sort	Maria Andreadaki
title	Sequential Membrane Rupture and Vesiculation during Plasmodium berghei Gametocyte Egress from the Red Blood Cell
title_short	Sequential Membrane Rupture and Vesiculation during Plasmodium berghei Gametocyte Egress from the Red Blood Cell
title_full	Sequential Membrane Rupture and Vesiculation during Plasmodium berghei Gametocyte Egress from the Red Blood Cell
title_fullStr	Sequential Membrane Rupture and Vesiculation during Plasmodium berghei Gametocyte Egress from the Red Blood Cell
title_full_unstemmed	Sequential Membrane Rupture and Vesiculation during Plasmodium berghei Gametocyte Egress from the Red Blood Cell
title_sort	sequential membrane rupture and vesiculation during plasmodium berghei gametocyte egress from the red blood cell
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/f364a0dae290472993a2c088f8fb673d
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Sequential Membrane Rupture and Vesiculation during Plasmodium berghei Gametocyte Egress from the Red Blood Cell

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