Short Communication: Weak delineation of Styrax species growing in North Sumatra, Indonesia by matK + rbcL gene

Susilowati A, Hendalastuti H, Kholibrina CR, Ramadhani R. 2017. Weak delineation of Styrax species growing in North Sumatra, Indonesia by matK + rbcL gene. Biodiversitas 18: 1270-1274. The aim of DNA barcoding is to enable precise identification of species from analysis of a unique DNA sequence of a...

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Autores principales:	ARIDA SUSILOWATI, HENTI HENDALASTUTI, CUT RIZLANI KHOLIBRINA, RINI RAMADHANI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2017
Materias:	barcoding mat k rbcl gene styrax kemenyan Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f365ce3f7ed24fd3b131da6aa757c3ce
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spelling	oai:doaj.org-article:f365ce3f7ed24fd3b131da6aa757c3ce2021-11-16T13:56:34ZShort Communication: Weak delineation of Styrax species growing in North Sumatra, Indonesia by matK + rbcL gene1412-033X2085-472210.13057/biodiv/d180353https://doaj.org/article/f365ce3f7ed24fd3b131da6aa757c3ce2017-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2053https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Susilowati A, Hendalastuti H, Kholibrina CR, Ramadhani R. 2017. Weak delineation of Styrax species growing in North Sumatra, Indonesia by matK + rbcL gene. Biodiversitas 18: 1270-1274. The aim of DNA barcoding is to enable precise identification of species from analysis of a unique DNA sequence of a target gene. The present study was undertaken to develop barcodes for different species of Styrax L. growing in North Sumatra, Indonesia. Four putative species of Styrax (each represented by specimens collected from two districts in North Sumatra) were evaluated using two regions in the plastid genome (matK and rbcL) in order to discriminate them at species level. Results showed that matK yielded 891 bp after alignment, however there was no precise identification to species level. The matK gene only differentiated the four recognized species into two groups, each group consisted of two of the species. The rbcL gene yielded 588 bp and showed no variation between species: that is,it determined all the currently recognized species to be of one and the same haplotype. Furthermore, combined matK + rbcL gave a similar grouping result to that for the matK gene considered alone. Considering the overall performance of these loci, we suggest matK + rbcL is not strong enough to determine Styrax growing in North Sumatra to the species level as distinguished on morphological grounds. These findings show the necessity of finding other candidate genes or markers that can potentially be helpful in delineating the various species of Styrax growing in North Sumatra, as well as other related Styrax genera.ARIDA SUSILOWATIHENTI HENDALASTUTICUT RIZLANI KHOLIBRINARINI RAMADHANIMBI & UNS Soloarticlebarcodingmat k rbcl genestyraxkemenyanBiology (General)QH301-705.5ENBiodiversitas, Vol 18, Iss 3, Pp 1270-1274 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	barcoding mat k rbcl gene styrax kemenyan Biology (General) QH301-705.5
spellingShingle	barcoding mat k rbcl gene styrax kemenyan Biology (General) QH301-705.5 ARIDA SUSILOWATI HENTI HENDALASTUTI CUT RIZLANI KHOLIBRINA RINI RAMADHANI Short Communication: Weak delineation of Styrax species growing in North Sumatra, Indonesia by matK + rbcL gene
description	Susilowati A, Hendalastuti H, Kholibrina CR, Ramadhani R. 2017. Weak delineation of Styrax species growing in North Sumatra, Indonesia by matK + rbcL gene. Biodiversitas 18: 1270-1274. The aim of DNA barcoding is to enable precise identification of species from analysis of a unique DNA sequence of a target gene. The present study was undertaken to develop barcodes for different species of Styrax L. growing in North Sumatra, Indonesia. Four putative species of Styrax (each represented by specimens collected from two districts in North Sumatra) were evaluated using two regions in the plastid genome (matK and rbcL) in order to discriminate them at species level. Results showed that matK yielded 891 bp after alignment, however there was no precise identification to species level. The matK gene only differentiated the four recognized species into two groups, each group consisted of two of the species. The rbcL gene yielded 588 bp and showed no variation between species: that is,it determined all the currently recognized species to be of one and the same haplotype. Furthermore, combined matK + rbcL gave a similar grouping result to that for the matK gene considered alone. Considering the overall performance of these loci, we suggest matK + rbcL is not strong enough to determine Styrax growing in North Sumatra to the species level as distinguished on morphological grounds. These findings show the necessity of finding other candidate genes or markers that can potentially be helpful in delineating the various species of Styrax growing in North Sumatra, as well as other related Styrax genera.
format	article
author	ARIDA SUSILOWATI HENTI HENDALASTUTI CUT RIZLANI KHOLIBRINA RINI RAMADHANI
author_facet	ARIDA SUSILOWATI HENTI HENDALASTUTI CUT RIZLANI KHOLIBRINA RINI RAMADHANI
author_sort	ARIDA SUSILOWATI
title	Short Communication: Weak delineation of Styrax species growing in North Sumatra, Indonesia by matK + rbcL gene
title_short	Short Communication: Weak delineation of Styrax species growing in North Sumatra, Indonesia by matK + rbcL gene
title_full	Short Communication: Weak delineation of Styrax species growing in North Sumatra, Indonesia by matK + rbcL gene
title_fullStr	Short Communication: Weak delineation of Styrax species growing in North Sumatra, Indonesia by matK + rbcL gene
title_full_unstemmed	Short Communication: Weak delineation of Styrax species growing in North Sumatra, Indonesia by matK + rbcL gene
title_sort	short communication: weak delineation of styrax species growing in north sumatra, indonesia by matk + rbcl gene
publisher	MBI & UNS Solo
publishDate	2017
url	https://doaj.org/article/f365ce3f7ed24fd3b131da6aa757c3ce
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Short Communication: Weak delineation of Styrax species growing in North Sumatra, Indonesia by matK + rbcL gene

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