Metabolic Analysis of Schizochytrium Mutants With High DHA Content Achieved With ARTP Mutagenesis Combined With Iodoacetic Acid and Dehydroepiandrosterone Screening

High DHA production cost caused by low DHA titer and productivity of the current Schizochytrium strains is a bottleneck for its application in competition with traditional fish-oil based approach. In this study, atmospheric and room-temperature plasma with iodoacetic acid and dehydroepiandrosterone...

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Autores principales:	Lei Zeng, Yanqi Bi, Pengfei Guo, Yali Bi, Tiantian Wang, Liang Dong, Fangzhong Wang, Lei Chen, Weiwen Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	schizochytrium DHA metabolomic analysis ARTP mutagenesis iodoacetic Acid dehydroepiandrosterone Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/f36b5c4614e5410ba7e8188e9bf0fd3d
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spelling	oai:doaj.org-article:f36b5c4614e5410ba7e8188e9bf0fd3d2021-11-18T09:53:18ZMetabolic Analysis of Schizochytrium Mutants With High DHA Content Achieved With ARTP Mutagenesis Combined With Iodoacetic Acid and Dehydroepiandrosterone Screening2296-418510.3389/fbioe.2021.738052https://doaj.org/article/f36b5c4614e5410ba7e8188e9bf0fd3d2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.738052/fullhttps://doaj.org/toc/2296-4185High DHA production cost caused by low DHA titer and productivity of the current Schizochytrium strains is a bottleneck for its application in competition with traditional fish-oil based approach. In this study, atmospheric and room-temperature plasma with iodoacetic acid and dehydroepiandrosterone screening led to three mutants, 6–8, 6–16 and 6–23 all with increased growth and DHA accumulations. A LC/MS metabolomic analysis revealed the increased metabolism in PPP and EMP as well as the decreased TCA cycle might be relevant to the increased growth and DHA biosynthesis in the mutants. Finally, the mutant 6–23, which achieved the highest growth and DHA accumulation among all mutants, was evaluated in a 5 L fermentor. The results showed that the DHA concentration and productivity in mutant 6–23 were 41.4 g/L and 430.7 mg/L/h in fermentation for 96 h, respectively, which is the highest reported so far in literature. The study provides a novel strain improvement strategy for DHA-producing Schizochytrium.Lei ZengLei ZengLei ZengYanqi BiYanqi BiYanqi BiPengfei GuoPengfei GuoPengfei GuoYali BiYali BiYali BiTiantian WangTiantian WangTiantian WangLiang DongLiang DongLiang DongFangzhong WangFangzhong WangLei ChenLei ChenLei ChenWeiwen ZhangWeiwen ZhangWeiwen ZhangWeiwen ZhangFrontiers Media S.A.articleschizochytriumDHAmetabolomic analysisARTP mutagenesisiodoacetic AciddehydroepiandrosteroneBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	schizochytrium DHA metabolomic analysis ARTP mutagenesis iodoacetic Acid dehydroepiandrosterone Biotechnology TP248.13-248.65
spellingShingle	schizochytrium DHA metabolomic analysis ARTP mutagenesis iodoacetic Acid dehydroepiandrosterone Biotechnology TP248.13-248.65 Lei Zeng Lei Zeng Lei Zeng Yanqi Bi Yanqi Bi Yanqi Bi Pengfei Guo Pengfei Guo Pengfei Guo Yali Bi Yali Bi Yali Bi Tiantian Wang Tiantian Wang Tiantian Wang Liang Dong Liang Dong Liang Dong Fangzhong Wang Fangzhong Wang Lei Chen Lei Chen Lei Chen Weiwen Zhang Weiwen Zhang Weiwen Zhang Weiwen Zhang Metabolic Analysis of Schizochytrium Mutants With High DHA Content Achieved With ARTP Mutagenesis Combined With Iodoacetic Acid and Dehydroepiandrosterone Screening
description	High DHA production cost caused by low DHA titer and productivity of the current Schizochytrium strains is a bottleneck for its application in competition with traditional fish-oil based approach. In this study, atmospheric and room-temperature plasma with iodoacetic acid and dehydroepiandrosterone screening led to three mutants, 6–8, 6–16 and 6–23 all with increased growth and DHA accumulations. A LC/MS metabolomic analysis revealed the increased metabolism in PPP and EMP as well as the decreased TCA cycle might be relevant to the increased growth and DHA biosynthesis in the mutants. Finally, the mutant 6–23, which achieved the highest growth and DHA accumulation among all mutants, was evaluated in a 5 L fermentor. The results showed that the DHA concentration and productivity in mutant 6–23 were 41.4 g/L and 430.7 mg/L/h in fermentation for 96 h, respectively, which is the highest reported so far in literature. The study provides a novel strain improvement strategy for DHA-producing Schizochytrium.
format	article
author	Lei Zeng Lei Zeng Lei Zeng Yanqi Bi Yanqi Bi Yanqi Bi Pengfei Guo Pengfei Guo Pengfei Guo Yali Bi Yali Bi Yali Bi Tiantian Wang Tiantian Wang Tiantian Wang Liang Dong Liang Dong Liang Dong Fangzhong Wang Fangzhong Wang Lei Chen Lei Chen Lei Chen Weiwen Zhang Weiwen Zhang Weiwen Zhang Weiwen Zhang
author_facet	Lei Zeng Lei Zeng Lei Zeng Yanqi Bi Yanqi Bi Yanqi Bi Pengfei Guo Pengfei Guo Pengfei Guo Yali Bi Yali Bi Yali Bi Tiantian Wang Tiantian Wang Tiantian Wang Liang Dong Liang Dong Liang Dong Fangzhong Wang Fangzhong Wang Lei Chen Lei Chen Lei Chen Weiwen Zhang Weiwen Zhang Weiwen Zhang Weiwen Zhang
author_sort	Lei Zeng
title	Metabolic Analysis of Schizochytrium Mutants With High DHA Content Achieved With ARTP Mutagenesis Combined With Iodoacetic Acid and Dehydroepiandrosterone Screening
title_short	Metabolic Analysis of Schizochytrium Mutants With High DHA Content Achieved With ARTP Mutagenesis Combined With Iodoacetic Acid and Dehydroepiandrosterone Screening
title_full	Metabolic Analysis of Schizochytrium Mutants With High DHA Content Achieved With ARTP Mutagenesis Combined With Iodoacetic Acid and Dehydroepiandrosterone Screening
title_fullStr	Metabolic Analysis of Schizochytrium Mutants With High DHA Content Achieved With ARTP Mutagenesis Combined With Iodoacetic Acid and Dehydroepiandrosterone Screening
title_full_unstemmed	Metabolic Analysis of Schizochytrium Mutants With High DHA Content Achieved With ARTP Mutagenesis Combined With Iodoacetic Acid and Dehydroepiandrosterone Screening
title_sort	metabolic analysis of schizochytrium mutants with high dha content achieved with artp mutagenesis combined with iodoacetic acid and dehydroepiandrosterone screening
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f36b5c4614e5410ba7e8188e9bf0fd3d
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Metabolic Analysis of Schizochytrium Mutants With High DHA Content Achieved With ARTP Mutagenesis Combined With Iodoacetic Acid and Dehydroepiandrosterone Screening

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