Recording mobile DNA in the gut microbiota using an Escherichia coli CRISPR-Cas spacer acquisition platform

Horizontal gene transfer (HGT) in complex bacterial communities has been mostly studied using metagenomic analyses. Here, the authors develop an E. coli CRISPR-Cas spacer acquisition platform that allows real-time recording of HGT events at nucleotide-resolution, identifying diverse DNA transfer eve...

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Autores principales:	Christian Munck, Ravi U. Sheth, Daniel E. Freedberg, Harris H. Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/f36c8c6e1cf94ca1940242b3347cd40a
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id	oai:doaj.org-article:f36c8c6e1cf94ca1940242b3347cd40a
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spelling	oai:doaj.org-article:f36c8c6e1cf94ca1940242b3347cd40a2021-12-02T17:32:13ZRecording mobile DNA in the gut microbiota using an Escherichia coli CRISPR-Cas spacer acquisition platform10.1038/s41467-019-14012-52041-1723https://doaj.org/article/f36c8c6e1cf94ca1940242b3347cd40a2020-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-14012-5https://doaj.org/toc/2041-1723Horizontal gene transfer (HGT) in complex bacterial communities has been mostly studied using metagenomic analyses. Here, the authors develop an E. coli CRISPR-Cas spacer acquisition platform that allows real-time recording of HGT events at nucleotide-resolution, identifying diverse DNA transfer events in human clinical fecal samples.Christian MunckRavi U. ShethDaniel E. FreedbergHarris H. WangNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Christian Munck Ravi U. Sheth Daniel E. Freedberg Harris H. Wang Recording mobile DNA in the gut microbiota using an Escherichia coli CRISPR-Cas spacer acquisition platform
description	Horizontal gene transfer (HGT) in complex bacterial communities has been mostly studied using metagenomic analyses. Here, the authors develop an E. coli CRISPR-Cas spacer acquisition platform that allows real-time recording of HGT events at nucleotide-resolution, identifying diverse DNA transfer events in human clinical fecal samples.
format	article
author	Christian Munck Ravi U. Sheth Daniel E. Freedberg Harris H. Wang
author_facet	Christian Munck Ravi U. Sheth Daniel E. Freedberg Harris H. Wang
author_sort	Christian Munck
title	Recording mobile DNA in the gut microbiota using an Escherichia coli CRISPR-Cas spacer acquisition platform
title_short	Recording mobile DNA in the gut microbiota using an Escherichia coli CRISPR-Cas spacer acquisition platform
title_full	Recording mobile DNA in the gut microbiota using an Escherichia coli CRISPR-Cas spacer acquisition platform
title_fullStr	Recording mobile DNA in the gut microbiota using an Escherichia coli CRISPR-Cas spacer acquisition platform
title_full_unstemmed	Recording mobile DNA in the gut microbiota using an Escherichia coli CRISPR-Cas spacer acquisition platform
title_sort	recording mobile dna in the gut microbiota using an escherichia coli crispr-cas spacer acquisition platform
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/f36c8c6e1cf94ca1940242b3347cd40a
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Recording mobile DNA in the gut microbiota using an Escherichia coli CRISPR-Cas spacer acquisition platform

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