Conserved microsatellites in ants enable population genetic and colony pedigree studies across a wide range of species.

Broadly applicable polymorphic genetic markers are essential tools for population genetics, and different types of markers have been developed for this purpose. Microsatellites have been employed as particularly polymorphic markers for over 20 years. However, PCR primers for microsatellite loci are...

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Autores principales:	Ian A Butler, Kimberly Siletti, Peter R Oxley, Daniel J C Kronauer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f37580e1833c455392ccc390dab0845a
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spelling	oai:doaj.org-article:f37580e1833c455392ccc390dab0845a2021-11-25T05:59:47ZConserved microsatellites in ants enable population genetic and colony pedigree studies across a wide range of species.1932-620310.1371/journal.pone.0107334https://doaj.org/article/f37580e1833c455392ccc390dab0845a2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0107334https://doaj.org/toc/1932-6203Broadly applicable polymorphic genetic markers are essential tools for population genetics, and different types of markers have been developed for this purpose. Microsatellites have been employed as particularly polymorphic markers for over 20 years. However, PCR primers for microsatellite loci are often not useful outside the species for which they were designed. This implies that a new set of loci has to be identified and primers developed for every new study species. To overcome this constraint, we identified 45 conserved microsatellite loci based on the eight currently available ant genomes and designed primers for PCR amplification. Among these loci, we chose 24 for in-depth study in six species covering six different ant subfamilies. On average, 11.16 of these 24 loci were polymorphic and in Hardy-Weinberg equilibrium in any given species. The average number of alleles for these polymorphic loci within single populations of the different species was 4.59. This set of genetic markers will thus be useful for population genetic and colony pedigree studies across a wide range of ant species, supplementing the markers available for previously studied species and greatly facilitating the study of the many ant species lacking genetic markers. Our study shows that it is possible to develop microsatellite loci that are both conserved over a broad range of taxa, yet polymorphic within species. This should encourage researchers to develop similar tools for other large taxonomic groups.Ian A ButlerKimberly SilettiPeter R OxleyDaniel J C KronauerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e107334 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ian A Butler Kimberly Siletti Peter R Oxley Daniel J C Kronauer Conserved microsatellites in ants enable population genetic and colony pedigree studies across a wide range of species.
description	Broadly applicable polymorphic genetic markers are essential tools for population genetics, and different types of markers have been developed for this purpose. Microsatellites have been employed as particularly polymorphic markers for over 20 years. However, PCR primers for microsatellite loci are often not useful outside the species for which they were designed. This implies that a new set of loci has to be identified and primers developed for every new study species. To overcome this constraint, we identified 45 conserved microsatellite loci based on the eight currently available ant genomes and designed primers for PCR amplification. Among these loci, we chose 24 for in-depth study in six species covering six different ant subfamilies. On average, 11.16 of these 24 loci were polymorphic and in Hardy-Weinberg equilibrium in any given species. The average number of alleles for these polymorphic loci within single populations of the different species was 4.59. This set of genetic markers will thus be useful for population genetic and colony pedigree studies across a wide range of ant species, supplementing the markers available for previously studied species and greatly facilitating the study of the many ant species lacking genetic markers. Our study shows that it is possible to develop microsatellite loci that are both conserved over a broad range of taxa, yet polymorphic within species. This should encourage researchers to develop similar tools for other large taxonomic groups.
format	article
author	Ian A Butler Kimberly Siletti Peter R Oxley Daniel J C Kronauer
author_facet	Ian A Butler Kimberly Siletti Peter R Oxley Daniel J C Kronauer
author_sort	Ian A Butler
title	Conserved microsatellites in ants enable population genetic and colony pedigree studies across a wide range of species.
title_short	Conserved microsatellites in ants enable population genetic and colony pedigree studies across a wide range of species.
title_full	Conserved microsatellites in ants enable population genetic and colony pedigree studies across a wide range of species.
title_fullStr	Conserved microsatellites in ants enable population genetic and colony pedigree studies across a wide range of species.
title_full_unstemmed	Conserved microsatellites in ants enable population genetic and colony pedigree studies across a wide range of species.
title_sort	conserved microsatellites in ants enable population genetic and colony pedigree studies across a wide range of species.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/f37580e1833c455392ccc390dab0845a
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Conserved microsatellites in ants enable population genetic and colony pedigree studies across a wide range of species.

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