Granuloma encapsulation is a key factor for containing tuberculosis infection in minipigs.

A transthoracic infection involving a low dose of Mycobacterium tuberculosis has been used to establish a new model of infection in minipigs. The 20-week monitoring period showed a marked Th1 response and poor humoral response for the whole infection. A detailed histopathological analysis was perfor...

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Autores principales: Olga Gil, Ivan Díaz, Cristina Vilaplana, Gustavo Tapia, Jorge Díaz, María Fort, Neus Cáceres, Sergio Pinto, Joan Caylà, Leigh Corner, Mariano Domingo, Pere-Joan Cardona
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Publicado: Public Library of Science (PLoS) 2010
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spelling oai:doaj.org-article:f3771f4bc1be48948f022a43d9ba87052021-11-25T06:24:46ZGranuloma encapsulation is a key factor for containing tuberculosis infection in minipigs.1932-620310.1371/journal.pone.0010030https://doaj.org/article/f3771f4bc1be48948f022a43d9ba87052010-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20386605/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203A transthoracic infection involving a low dose of Mycobacterium tuberculosis has been used to establish a new model of infection in minipigs. The 20-week monitoring period showed a marked Th1 response and poor humoral response for the whole infection. A detailed histopathological analysis was performed after slicing the formalin-fixed whole lungs of each animal. All lesions were recorded and classified according to their microscopic aspect, their relationship with the intralobular connective network and their degree of maturity in order to obtain a dissemination ratio (DR) between recent and old lesions. CFU counts and evolution of the DR with time showed that the proposed model correlated with a contained infection, decreasing from week 9 onwards. These findings suggest that the infection induces an initial Th1 response, which is followed by local fibrosis and encapsulation of the granulomas, thereby decreasing the onset of new lesions. Two therapeutic strategies were applied in order to understand how they could influence the model. Thus, chemotherapy with isoniazid alone helped to decrease the total number of lesions, despite the increase in DR after week 9, with similar kinetics to those of the control group, whereas addition of a therapeutic M. tuberculosis fragment-based vaccine after chemotherapy increased the Th1 and humoral responses, as well as the number of lesions, but decreased the DR. By providing a local pulmonary structure similar to that in humans, the mini-pig model highlights new aspects that could be key to a better understanding tuberculosis infection control in humans.Olga GilIvan DíazCristina VilaplanaGustavo TapiaJorge DíazMaría FortNeus CáceresSergio PintoJoan CaylàLeigh CornerMariano DomingoPere-Joan CardonaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 4, p e10030 (2010)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Olga Gil
Ivan Díaz
Cristina Vilaplana
Gustavo Tapia
Jorge Díaz
María Fort
Neus Cáceres
Sergio Pinto
Joan Caylà
Leigh Corner
Mariano Domingo
Pere-Joan Cardona
Granuloma encapsulation is a key factor for containing tuberculosis infection in minipigs.
description A transthoracic infection involving a low dose of Mycobacterium tuberculosis has been used to establish a new model of infection in minipigs. The 20-week monitoring period showed a marked Th1 response and poor humoral response for the whole infection. A detailed histopathological analysis was performed after slicing the formalin-fixed whole lungs of each animal. All lesions were recorded and classified according to their microscopic aspect, their relationship with the intralobular connective network and their degree of maturity in order to obtain a dissemination ratio (DR) between recent and old lesions. CFU counts and evolution of the DR with time showed that the proposed model correlated with a contained infection, decreasing from week 9 onwards. These findings suggest that the infection induces an initial Th1 response, which is followed by local fibrosis and encapsulation of the granulomas, thereby decreasing the onset of new lesions. Two therapeutic strategies were applied in order to understand how they could influence the model. Thus, chemotherapy with isoniazid alone helped to decrease the total number of lesions, despite the increase in DR after week 9, with similar kinetics to those of the control group, whereas addition of a therapeutic M. tuberculosis fragment-based vaccine after chemotherapy increased the Th1 and humoral responses, as well as the number of lesions, but decreased the DR. By providing a local pulmonary structure similar to that in humans, the mini-pig model highlights new aspects that could be key to a better understanding tuberculosis infection control in humans.
format article
author Olga Gil
Ivan Díaz
Cristina Vilaplana
Gustavo Tapia
Jorge Díaz
María Fort
Neus Cáceres
Sergio Pinto
Joan Caylà
Leigh Corner
Mariano Domingo
Pere-Joan Cardona
author_facet Olga Gil
Ivan Díaz
Cristina Vilaplana
Gustavo Tapia
Jorge Díaz
María Fort
Neus Cáceres
Sergio Pinto
Joan Caylà
Leigh Corner
Mariano Domingo
Pere-Joan Cardona
author_sort Olga Gil
title Granuloma encapsulation is a key factor for containing tuberculosis infection in minipigs.
title_short Granuloma encapsulation is a key factor for containing tuberculosis infection in minipigs.
title_full Granuloma encapsulation is a key factor for containing tuberculosis infection in minipigs.
title_fullStr Granuloma encapsulation is a key factor for containing tuberculosis infection in minipigs.
title_full_unstemmed Granuloma encapsulation is a key factor for containing tuberculosis infection in minipigs.
title_sort granuloma encapsulation is a key factor for containing tuberculosis infection in minipigs.
publisher Public Library of Science (PLoS)
publishDate 2010
url https://doaj.org/article/f3771f4bc1be48948f022a43d9ba8705
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