Conjugated C-6 hydroxylated bile acids in serum relate to human metabolic health and gut Clostridia species
Abstract Knowledge about in vivo effects of human circulating C-6 hydroxylated bile acids (BAs), also called muricholic acids, is sparse. It is unsettled if the gut microbiome might contribute to their biosynthesis. Here, we measured a range of serum BAs and related them to markers of human metaboli...
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Nature Portfolio
2021
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oai:doaj.org-article:f377899836e24ff2b751db0c731050412021-12-02T17:12:21ZConjugated C-6 hydroxylated bile acids in serum relate to human metabolic health and gut Clostridia species10.1038/s41598-021-91482-y2045-2322https://doaj.org/article/f377899836e24ff2b751db0c731050412021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91482-yhttps://doaj.org/toc/2045-2322Abstract Knowledge about in vivo effects of human circulating C-6 hydroxylated bile acids (BAs), also called muricholic acids, is sparse. It is unsettled if the gut microbiome might contribute to their biosynthesis. Here, we measured a range of serum BAs and related them to markers of human metabolic health and the gut microbiome. We examined 283 non-obese and obese Danish adults from the MetaHit study. Fasting concentrations of serum BAs were quantified using ultra-performance liquid chromatography-tandem mass-spectrometry. The gut microbiome was characterized with shotgun metagenomic sequencing and genome-scale metabolic modeling. We find that tauro- and glycohyocholic acid correlated inversely with body mass index (P = 4.1e-03, P = 1.9e-05, respectively), waist circumference (P = 0.017, P = 1.1e-04, respectively), body fat percentage (P = 2.5e-03, P = 2.3e-06, respectively), insulin resistance (P = 0.051, P = 4.6e-4, respectively), fasting concentrations of triglycerides (P = 0.06, P = 9.2e-4, respectively) and leptin (P = 0.067, P = 9.2e-4). Tauro- and glycohyocholic acids, and tauro-a-muricholic acid were directly linked with a distinct gut microbial community primarily composed of Clostridia species (P = 0.037, P = 0.013, P = 0.027, respectively). We conclude that serum conjugated C-6-hydroxylated BAs associate with measures of human metabolic health and gut communities of Clostridia species. The findings merit preclinical interventions and human feasibility studies to explore the therapeutic potential of these BAs in obesity and type 2 diabetes.Anders Ø PetersenHanna JulienneTuulia HyötyläinenPartho SenYong FanHelle Krogh PedersenSirkku JänttiTue H. HansenTrine NielsenTorben JørgensenTorben HansenPernille Neve MyersH. Bjørn NielsenS. Dusko EhrlichMatej OrešičOluf PedersenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
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DOAJ |
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DOAJ |
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EN |
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Medicine R Science Q |
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Medicine R Science Q Anders Ø Petersen Hanna Julienne Tuulia Hyötyläinen Partho Sen Yong Fan Helle Krogh Pedersen Sirkku Jäntti Tue H. Hansen Trine Nielsen Torben Jørgensen Torben Hansen Pernille Neve Myers H. Bjørn Nielsen S. Dusko Ehrlich Matej Orešič Oluf Pedersen Conjugated C-6 hydroxylated bile acids in serum relate to human metabolic health and gut Clostridia species |
| description |
Abstract Knowledge about in vivo effects of human circulating C-6 hydroxylated bile acids (BAs), also called muricholic acids, is sparse. It is unsettled if the gut microbiome might contribute to their biosynthesis. Here, we measured a range of serum BAs and related them to markers of human metabolic health and the gut microbiome. We examined 283 non-obese and obese Danish adults from the MetaHit study. Fasting concentrations of serum BAs were quantified using ultra-performance liquid chromatography-tandem mass-spectrometry. The gut microbiome was characterized with shotgun metagenomic sequencing and genome-scale metabolic modeling. We find that tauro- and glycohyocholic acid correlated inversely with body mass index (P = 4.1e-03, P = 1.9e-05, respectively), waist circumference (P = 0.017, P = 1.1e-04, respectively), body fat percentage (P = 2.5e-03, P = 2.3e-06, respectively), insulin resistance (P = 0.051, P = 4.6e-4, respectively), fasting concentrations of triglycerides (P = 0.06, P = 9.2e-4, respectively) and leptin (P = 0.067, P = 9.2e-4). Tauro- and glycohyocholic acids, and tauro-a-muricholic acid were directly linked with a distinct gut microbial community primarily composed of Clostridia species (P = 0.037, P = 0.013, P = 0.027, respectively). We conclude that serum conjugated C-6-hydroxylated BAs associate with measures of human metabolic health and gut communities of Clostridia species. The findings merit preclinical interventions and human feasibility studies to explore the therapeutic potential of these BAs in obesity and type 2 diabetes. |
| format |
article |
| author |
Anders Ø Petersen Hanna Julienne Tuulia Hyötyläinen Partho Sen Yong Fan Helle Krogh Pedersen Sirkku Jäntti Tue H. Hansen Trine Nielsen Torben Jørgensen Torben Hansen Pernille Neve Myers H. Bjørn Nielsen S. Dusko Ehrlich Matej Orešič Oluf Pedersen |
| author_facet |
Anders Ø Petersen Hanna Julienne Tuulia Hyötyläinen Partho Sen Yong Fan Helle Krogh Pedersen Sirkku Jäntti Tue H. Hansen Trine Nielsen Torben Jørgensen Torben Hansen Pernille Neve Myers H. Bjørn Nielsen S. Dusko Ehrlich Matej Orešič Oluf Pedersen |
| author_sort |
Anders Ø Petersen |
| title |
Conjugated C-6 hydroxylated bile acids in serum relate to human metabolic health and gut Clostridia species |
| title_short |
Conjugated C-6 hydroxylated bile acids in serum relate to human metabolic health and gut Clostridia species |
| title_full |
Conjugated C-6 hydroxylated bile acids in serum relate to human metabolic health and gut Clostridia species |
| title_fullStr |
Conjugated C-6 hydroxylated bile acids in serum relate to human metabolic health and gut Clostridia species |
| title_full_unstemmed |
Conjugated C-6 hydroxylated bile acids in serum relate to human metabolic health and gut Clostridia species |
| title_sort |
conjugated c-6 hydroxylated bile acids in serum relate to human metabolic health and gut clostridia species |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/f377899836e24ff2b751db0c73105041 |
| work_keys_str_mv |
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