Raman Imaging and Fluorescence Lifetime Imaging Microscopy for Diagnosis of Cancer State and Metabolic Monitoring

Hurdles for effective tumor therapy are delayed detection and limited effectiveness of systemic drug therapies by patient-specific multidrug resistance. Non-invasive bioimaging tools such as fluorescence lifetime imaging microscopy (FLIM) and Raman-microspectroscopy have evolved over the last decade...

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Autores principales:	Lucas Becker, Nicole Janssen, Shannon L. Layland, Thomas E. Mürdter, Anne T. Nies, Katja Schenke-Layland, Julia Marzi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Raman microspectroscopy fluorescence lifetime imaging microscopy tissue diagnostics tumor metabolism in situ imaging 3D in vitro models Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/f3783b53e972490ba242bdd84e494792
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spelling	oai:doaj.org-article:f3783b53e972490ba242bdd84e4947922021-11-25T17:02:36ZRaman Imaging and Fluorescence Lifetime Imaging Microscopy for Diagnosis of Cancer State and Metabolic Monitoring10.3390/cancers132256822072-6694https://doaj.org/article/f3783b53e972490ba242bdd84e4947922021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/22/5682https://doaj.org/toc/2072-6694Hurdles for effective tumor therapy are delayed detection and limited effectiveness of systemic drug therapies by patient-specific multidrug resistance. Non-invasive bioimaging tools such as fluorescence lifetime imaging microscopy (FLIM) and Raman-microspectroscopy have evolved over the last decade, providing the potential to be translated into clinics for early-stage disease detection, in vitro drug screening, and drug efficacy studies in personalized medicine. Accessing tissue- and cell-specific spectral signatures, Raman microspectroscopy has emerged as a diagnostic tool to identify precancerous lesions, cancer stages, or cell malignancy. In vivo Raman measurements have been enabled by recent technological advances in Raman endoscopy and signal-enhancing setups such as coherent anti-stokes Raman spectroscopy or surface-enhanced Raman spectroscopy. FLIM enables in situ investigations of metabolic processes such as glycolysis, oxidative stress, or mitochondrial activity by using the autofluorescence of co-enzymes NADH and FAD, which are associated with intrinsic proteins as a direct measure of tumor metabolism, cell death stages and drug efficacy. The combination of non-invasive and molecular-sensitive in situ techniques and advanced 3D tumor models such as patient-derived organoids or microtumors allows the recapitulation of tumor physiology and metabolism in vitro and facilitates the screening for patient-individualized drug treatment options.Lucas BeckerNicole JanssenShannon L. LaylandThomas E. MürdterAnne T. NiesKatja Schenke-LaylandJulia MarziMDPI AGarticleRaman microspectroscopyfluorescence lifetime imaging microscopytissue diagnosticstumor metabolismin situ imaging3D in vitro modelsNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5682, p 5682 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Raman microspectroscopy fluorescence lifetime imaging microscopy tissue diagnostics tumor metabolism in situ imaging 3D in vitro models Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Raman microspectroscopy fluorescence lifetime imaging microscopy tissue diagnostics tumor metabolism in situ imaging 3D in vitro models Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Lucas Becker Nicole Janssen Shannon L. Layland Thomas E. Mürdter Anne T. Nies Katja Schenke-Layland Julia Marzi Raman Imaging and Fluorescence Lifetime Imaging Microscopy for Diagnosis of Cancer State and Metabolic Monitoring
description	Hurdles for effective tumor therapy are delayed detection and limited effectiveness of systemic drug therapies by patient-specific multidrug resistance. Non-invasive bioimaging tools such as fluorescence lifetime imaging microscopy (FLIM) and Raman-microspectroscopy have evolved over the last decade, providing the potential to be translated into clinics for early-stage disease detection, in vitro drug screening, and drug efficacy studies in personalized medicine. Accessing tissue- and cell-specific spectral signatures, Raman microspectroscopy has emerged as a diagnostic tool to identify precancerous lesions, cancer stages, or cell malignancy. In vivo Raman measurements have been enabled by recent technological advances in Raman endoscopy and signal-enhancing setups such as coherent anti-stokes Raman spectroscopy or surface-enhanced Raman spectroscopy. FLIM enables in situ investigations of metabolic processes such as glycolysis, oxidative stress, or mitochondrial activity by using the autofluorescence of co-enzymes NADH and FAD, which are associated with intrinsic proteins as a direct measure of tumor metabolism, cell death stages and drug efficacy. The combination of non-invasive and molecular-sensitive in situ techniques and advanced 3D tumor models such as patient-derived organoids or microtumors allows the recapitulation of tumor physiology and metabolism in vitro and facilitates the screening for patient-individualized drug treatment options.
format	article
author	Lucas Becker Nicole Janssen Shannon L. Layland Thomas E. Mürdter Anne T. Nies Katja Schenke-Layland Julia Marzi
author_facet	Lucas Becker Nicole Janssen Shannon L. Layland Thomas E. Mürdter Anne T. Nies Katja Schenke-Layland Julia Marzi
author_sort	Lucas Becker
title	Raman Imaging and Fluorescence Lifetime Imaging Microscopy for Diagnosis of Cancer State and Metabolic Monitoring
title_short	Raman Imaging and Fluorescence Lifetime Imaging Microscopy for Diagnosis of Cancer State and Metabolic Monitoring
title_full	Raman Imaging and Fluorescence Lifetime Imaging Microscopy for Diagnosis of Cancer State and Metabolic Monitoring
title_fullStr	Raman Imaging and Fluorescence Lifetime Imaging Microscopy for Diagnosis of Cancer State and Metabolic Monitoring
title_full_unstemmed	Raman Imaging and Fluorescence Lifetime Imaging Microscopy for Diagnosis of Cancer State and Metabolic Monitoring
title_sort	raman imaging and fluorescence lifetime imaging microscopy for diagnosis of cancer state and metabolic monitoring
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f3783b53e972490ba242bdd84e494792
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Raman Imaging and Fluorescence Lifetime Imaging Microscopy for Diagnosis of Cancer State and Metabolic Monitoring

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