Directed evolution of Metarhizium fungus improves its biocontrol efficacy against Varroa mites in honey bee colonies

Abstract Entomopathogenic fungi show great promise as pesticides in terms of their relatively high target specificity, low non-target toxicity, and low residual effects in agricultural fields and the environment. However, they also frequently have characteristics that limit their use, especially con...

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Autores principales:	Jennifer O. Han, Nicholas L. Naeger, Brandon K. Hopkins, David Sumerlin, Paul E. Stamets, Lori M. Carris, Walter S. Sheppard
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f37cb6f499cc405fa9ac9fe98f9744b3
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spelling	oai:doaj.org-article:f37cb6f499cc405fa9ac9fe98f9744b32021-12-02T16:51:20ZDirected evolution of Metarhizium fungus improves its biocontrol efficacy against Varroa mites in honey bee colonies10.1038/s41598-021-89811-22045-2322https://doaj.org/article/f37cb6f499cc405fa9ac9fe98f9744b32021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89811-2https://doaj.org/toc/2045-2322Abstract Entomopathogenic fungi show great promise as pesticides in terms of their relatively high target specificity, low non-target toxicity, and low residual effects in agricultural fields and the environment. However, they also frequently have characteristics that limit their use, especially concerning tolerances to temperature, ultraviolet radiation, or other abiotic factors. The devastating ectoparasite of honey bees, Varroa destructor, is susceptible to entomopathogenic fungi, but the relatively warm temperatures inside honey bee hives have prevented these fungi from becoming effective control measures. Using a combination of traditional selection and directed evolution techniques developed for this system, new strains of Metarhizium brunneum were created that survived, germinated, and grew better at bee hive temperatures (35 °C). Field tests with full-sized honey bee colonies confirmed that the new strain JH1078 is more virulent against Varroa mites and controls the pest comparable to current treatments. These results indicate that entomopathogenic fungi are evolutionarily labile and capable of playing a larger role in modern pest management practices.Jennifer O. HanNicholas L. NaegerBrandon K. HopkinsDavid SumerlinPaul E. StametsLori M. CarrisWalter S. SheppardNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jennifer O. Han Nicholas L. Naeger Brandon K. Hopkins David Sumerlin Paul E. Stamets Lori M. Carris Walter S. Sheppard Directed evolution of Metarhizium fungus improves its biocontrol efficacy against Varroa mites in honey bee colonies
description	Abstract Entomopathogenic fungi show great promise as pesticides in terms of their relatively high target specificity, low non-target toxicity, and low residual effects in agricultural fields and the environment. However, they also frequently have characteristics that limit their use, especially concerning tolerances to temperature, ultraviolet radiation, or other abiotic factors. The devastating ectoparasite of honey bees, Varroa destructor, is susceptible to entomopathogenic fungi, but the relatively warm temperatures inside honey bee hives have prevented these fungi from becoming effective control measures. Using a combination of traditional selection and directed evolution techniques developed for this system, new strains of Metarhizium brunneum were created that survived, germinated, and grew better at bee hive temperatures (35 °C). Field tests with full-sized honey bee colonies confirmed that the new strain JH1078 is more virulent against Varroa mites and controls the pest comparable to current treatments. These results indicate that entomopathogenic fungi are evolutionarily labile and capable of playing a larger role in modern pest management practices.
format	article
author	Jennifer O. Han Nicholas L. Naeger Brandon K. Hopkins David Sumerlin Paul E. Stamets Lori M. Carris Walter S. Sheppard
author_facet	Jennifer O. Han Nicholas L. Naeger Brandon K. Hopkins David Sumerlin Paul E. Stamets Lori M. Carris Walter S. Sheppard
author_sort	Jennifer O. Han
title	Directed evolution of Metarhizium fungus improves its biocontrol efficacy against Varroa mites in honey bee colonies
title_short	Directed evolution of Metarhizium fungus improves its biocontrol efficacy against Varroa mites in honey bee colonies
title_full	Directed evolution of Metarhizium fungus improves its biocontrol efficacy against Varroa mites in honey bee colonies
title_fullStr	Directed evolution of Metarhizium fungus improves its biocontrol efficacy against Varroa mites in honey bee colonies
title_full_unstemmed	Directed evolution of Metarhizium fungus improves its biocontrol efficacy against Varroa mites in honey bee colonies
title_sort	directed evolution of metarhizium fungus improves its biocontrol efficacy against varroa mites in honey bee colonies
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f37cb6f499cc405fa9ac9fe98f9744b3
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Directed evolution of Metarhizium fungus improves its biocontrol efficacy against Varroa mites in honey bee colonies

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