Isolation, characterization, activity test and molecular identification of thermophilic bacteria producing proteases from Dolok Tinggi Raja Natural Hot Springs, North Sumatra, Indonesia

Abstract. Fachrial E, Krisdianilo V, Harmileni, Lister INE, Nugroho TT, Saryono. 2021. Isolation, characterization, activity test and molecular identification of thermophilic bacteria producing proteases from Dolok Tinggi Raja Natural Hot Springs, North Sumatra, Indonesia. Biodiversitas 22: 1725-173...

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Autores principales:	Edy Fachrial, VISENSIUS KRISDIANILO, Harmileni HARMILENI, I Nyoman Ehrich Lister, Titania T Nugroho, Saryono SARYONO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	bacteria, enzymes, molecular identification, protease ,thermophilic. Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f380d16797f842f39cd7e7d4fbad48fb
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spelling	oai:doaj.org-article:f380d16797f842f39cd7e7d4fbad48fb2021-11-22T00:57:38ZIsolation, characterization, activity test and molecular identification of thermophilic bacteria producing proteases from Dolok Tinggi Raja Natural Hot Springs, North Sumatra, Indonesia1412-033X2085-472210.13057/biodiv/d220416https://doaj.org/article/f380d16797f842f39cd7e7d4fbad48fb2021-03-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7832https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Fachrial E, Krisdianilo V, Harmileni, Lister INE, Nugroho TT, Saryono. 2021. Isolation, characterization, activity test and molecular identification of thermophilic bacteria producing proteases from Dolok Tinggi Raja Natural Hot Springs, North Sumatra, Indonesia. Biodiversitas 22: 1725-1732. Proteases are complex enzymes that can be applied in both commercial products and physiological fields. This enzyme accelerates the breakdown of peptide bonds in polypeptides and proteins using hydrolysis reactions, transforming them into simpler bonds. In recent years, the demand for protease enzymes in Indonesia is still dependent on imported products, and there has also been an increase in their use. This is because there are many advantages in terms of efficiency, environmental friendliness, and its ability to catalyze a reaction without by-products. Proteases dominate the enzyme market with a total of 70% sales. Furthermore, they are manufactured from thermophilic bacteria as a solution to the problem of enzyme use in industries, where enzymes are easily damaged at high temperatures. The thermophilic protease enzyme can be found in thermophilic microorganisms living in extreme temperatures such as hot springs. There are lots of mountainous areas in North Sumatra Province, Indonesia with many hot springs. For example, the Dolok Tinggi Raja Natural Hot Springs of Simalungun District which has a water temperature of 50oC with a pH of about 6, the potential of which still needs to be explored. This study aims to isolate and determine the activity and molecular structure of thermophilic bacteria producing protease in Dolok Tinggi Raja Natural Hot Springs. Protease activity was determined based on the formation of clear zones in Skim Milk Agar medium. In addition, a total of 20 isolates were successfully obtained from sediment and water. Each was tested biochemically and morphologically, where 19 isolates had the activity of proteases, with the highest in isolate UTMTR VK S9 of 23.67 U/mL. Molecular identification was performed by the amplification of 16SrRNA gene based on BLAST and phylogenic analysis with MEGA X. Phylogenic results showed that isolate UTMTR VK S9 possessed similarities to Bacillus paralicheniformis strain A30103. Meanwhile, more research is required to purify enzymes for application in the industrial world.Edy FachrialVISENSIUS KRISDIANILOHarmileni HARMILENII Nyoman Ehrich ListerTitania T NugrohoSaryono SARYONOMBI & UNS Soloarticlebacteria, enzymes, molecular identification, protease ,thermophilic.Biology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteria, enzymes, molecular identification, protease ,thermophilic. Biology (General) QH301-705.5
spellingShingle	bacteria, enzymes, molecular identification, protease ,thermophilic. Biology (General) QH301-705.5 Edy Fachrial VISENSIUS KRISDIANILO Harmileni HARMILENI I Nyoman Ehrich Lister Titania T Nugroho Saryono SARYONO Isolation, characterization, activity test and molecular identification of thermophilic bacteria producing proteases from Dolok Tinggi Raja Natural Hot Springs, North Sumatra, Indonesia
description	Abstract. Fachrial E, Krisdianilo V, Harmileni, Lister INE, Nugroho TT, Saryono. 2021. Isolation, characterization, activity test and molecular identification of thermophilic bacteria producing proteases from Dolok Tinggi Raja Natural Hot Springs, North Sumatra, Indonesia. Biodiversitas 22: 1725-1732. Proteases are complex enzymes that can be applied in both commercial products and physiological fields. This enzyme accelerates the breakdown of peptide bonds in polypeptides and proteins using hydrolysis reactions, transforming them into simpler bonds. In recent years, the demand for protease enzymes in Indonesia is still dependent on imported products, and there has also been an increase in their use. This is because there are many advantages in terms of efficiency, environmental friendliness, and its ability to catalyze a reaction without by-products. Proteases dominate the enzyme market with a total of 70% sales. Furthermore, they are manufactured from thermophilic bacteria as a solution to the problem of enzyme use in industries, where enzymes are easily damaged at high temperatures. The thermophilic protease enzyme can be found in thermophilic microorganisms living in extreme temperatures such as hot springs. There are lots of mountainous areas in North Sumatra Province, Indonesia with many hot springs. For example, the Dolok Tinggi Raja Natural Hot Springs of Simalungun District which has a water temperature of 50oC with a pH of about 6, the potential of which still needs to be explored. This study aims to isolate and determine the activity and molecular structure of thermophilic bacteria producing protease in Dolok Tinggi Raja Natural Hot Springs. Protease activity was determined based on the formation of clear zones in Skim Milk Agar medium. In addition, a total of 20 isolates were successfully obtained from sediment and water. Each was tested biochemically and morphologically, where 19 isolates had the activity of proteases, with the highest in isolate UTMTR VK S9 of 23.67 U/mL. Molecular identification was performed by the amplification of 16SrRNA gene based on BLAST and phylogenic analysis with MEGA X. Phylogenic results showed that isolate UTMTR VK S9 possessed similarities to Bacillus paralicheniformis strain A30103. Meanwhile, more research is required to purify enzymes for application in the industrial world.
format	article
author	Edy Fachrial VISENSIUS KRISDIANILO Harmileni HARMILENI I Nyoman Ehrich Lister Titania T Nugroho Saryono SARYONO
author_facet	Edy Fachrial VISENSIUS KRISDIANILO Harmileni HARMILENI I Nyoman Ehrich Lister Titania T Nugroho Saryono SARYONO
author_sort	Edy Fachrial
title	Isolation, characterization, activity test and molecular identification of thermophilic bacteria producing proteases from Dolok Tinggi Raja Natural Hot Springs, North Sumatra, Indonesia
title_short	Isolation, characterization, activity test and molecular identification of thermophilic bacteria producing proteases from Dolok Tinggi Raja Natural Hot Springs, North Sumatra, Indonesia
title_full	Isolation, characterization, activity test and molecular identification of thermophilic bacteria producing proteases from Dolok Tinggi Raja Natural Hot Springs, North Sumatra, Indonesia
title_fullStr	Isolation, characterization, activity test and molecular identification of thermophilic bacteria producing proteases from Dolok Tinggi Raja Natural Hot Springs, North Sumatra, Indonesia
title_full_unstemmed	Isolation, characterization, activity test and molecular identification of thermophilic bacteria producing proteases from Dolok Tinggi Raja Natural Hot Springs, North Sumatra, Indonesia
title_sort	isolation, characterization, activity test and molecular identification of thermophilic bacteria producing proteases from dolok tinggi raja natural hot springs, north sumatra, indonesia
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/f380d16797f842f39cd7e7d4fbad48fb
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Isolation, characterization, activity test and molecular identification of thermophilic bacteria producing proteases from Dolok Tinggi Raja Natural Hot Springs, North Sumatra, Indonesia

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