Whole body potassium as a biomarker for potassium uptake using a mouse model

Abstract Potassium is known for its effect on modifiable chronic diseases like hypertension, cardiac disease, diabetes (type-2), and bone health. In this study, a new method, neutron generator based neutron activation analysis (NAA), was utilized to measure potassium (K) in mouse carcasses. A DD110...

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Autores principales:	Sana Tabbassum, Pinjing Cheng, Frank M. Yanko, Rekha Balachandran, Michael Aschner, Aaron B. Bowman, Linda H. Nie
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f3810f8064d1477eae0af8d2b253f9e0
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spelling	oai:doaj.org-article:f3810f8064d1477eae0af8d2b253f9e02021-12-02T13:17:42ZWhole body potassium as a biomarker for potassium uptake using a mouse model10.1038/s41598-021-85233-22045-2322https://doaj.org/article/f3810f8064d1477eae0af8d2b253f9e02021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85233-2https://doaj.org/toc/2045-2322Abstract Potassium is known for its effect on modifiable chronic diseases like hypertension, cardiac disease, diabetes (type-2), and bone health. In this study, a new method, neutron generator based neutron activation analysis (NAA), was utilized to measure potassium (K) in mouse carcasses. A DD110 neutron generator based NAA assembly was used for irradiation.Thirty-two postmortem mice (n= 16 males and 16 females, average weight $$22.02\pm 1.3$$ 22.02 ± 1.3 and $$17.9\pm 1.1$$ 17.9 ± 1.1 g) were employed for this study. Soft-tissue equivalent mouse phantoms were prepared for the calibration. All mice were irradiated for 10 minutes, and the gamma spectrum with 42K was collected using a high efficiency, high purity germanium (HPGe) detector. A lead shielding assembly was designed and developed around the HPGe detector to obtain an improved detection limit. Each mouse sample was irradiated and measured twice to reduce uncertainty. The average potassium concentration was found to be significantly higher in males $$(2846 \pm 525 \upmu g/g)$$ ( 2846 ± 525 μ g / g ) compared to females $$(2116.2 \pm 432 \upmu g/g)$$ ( 2116.2 ± 432 μ g / g ) . We also observed a significant correlation between potassium concentration and the weight of the mice. The detection limit for potassium quantification with the NAA system was 46 ppm. The radiation dose to the mouse was approximately 56 $${ \pm 1.6 }$$ ± 1.6 mSv for 10-min irradiation. In conclusion, this method is suitable for estimating individual potassium concentration in small animals. The direct evaluation of total body potassium in small animals provides a new way to estimate potassium uptake in animal models. This method can be adapted later to quantify potassium in the human hand and small animals in vivo. When used in vivo, it is also expected to be a valuable tool for longitudinal assessment, kinetics, and health outcomes.Sana TabbassumPinjing ChengFrank M. YankoRekha BalachandranMichael AschnerAaron B. BowmanLinda H. NieNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sana Tabbassum Pinjing Cheng Frank M. Yanko Rekha Balachandran Michael Aschner Aaron B. Bowman Linda H. Nie Whole body potassium as a biomarker for potassium uptake using a mouse model
description	Abstract Potassium is known for its effect on modifiable chronic diseases like hypertension, cardiac disease, diabetes (type-2), and bone health. In this study, a new method, neutron generator based neutron activation analysis (NAA), was utilized to measure potassium (K) in mouse carcasses. A DD110 neutron generator based NAA assembly was used for irradiation.Thirty-two postmortem mice (n= 16 males and 16 females, average weight $$22.02\pm 1.3$$ 22.02 ± 1.3 and $$17.9\pm 1.1$$ 17.9 ± 1.1 g) were employed for this study. Soft-tissue equivalent mouse phantoms were prepared for the calibration. All mice were irradiated for 10 minutes, and the gamma spectrum with 42K was collected using a high efficiency, high purity germanium (HPGe) detector. A lead shielding assembly was designed and developed around the HPGe detector to obtain an improved detection limit. Each mouse sample was irradiated and measured twice to reduce uncertainty. The average potassium concentration was found to be significantly higher in males $$(2846 \pm 525 \upmu g/g)$$ ( 2846 ± 525 μ g / g ) compared to females $$(2116.2 \pm 432 \upmu g/g)$$ ( 2116.2 ± 432 μ g / g ) . We also observed a significant correlation between potassium concentration and the weight of the mice. The detection limit for potassium quantification with the NAA system was 46 ppm. The radiation dose to the mouse was approximately 56 $${ \pm 1.6 }$$ ± 1.6 mSv for 10-min irradiation. In conclusion, this method is suitable for estimating individual potassium concentration in small animals. The direct evaluation of total body potassium in small animals provides a new way to estimate potassium uptake in animal models. This method can be adapted later to quantify potassium in the human hand and small animals in vivo. When used in vivo, it is also expected to be a valuable tool for longitudinal assessment, kinetics, and health outcomes.
format	article
author	Sana Tabbassum Pinjing Cheng Frank M. Yanko Rekha Balachandran Michael Aschner Aaron B. Bowman Linda H. Nie
author_facet	Sana Tabbassum Pinjing Cheng Frank M. Yanko Rekha Balachandran Michael Aschner Aaron B. Bowman Linda H. Nie
author_sort	Sana Tabbassum
title	Whole body potassium as a biomarker for potassium uptake using a mouse model
title_short	Whole body potassium as a biomarker for potassium uptake using a mouse model
title_full	Whole body potassium as a biomarker for potassium uptake using a mouse model
title_fullStr	Whole body potassium as a biomarker for potassium uptake using a mouse model
title_full_unstemmed	Whole body potassium as a biomarker for potassium uptake using a mouse model
title_sort	whole body potassium as a biomarker for potassium uptake using a mouse model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f3810f8064d1477eae0af8d2b253f9e0
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Whole body potassium as a biomarker for potassium uptake using a mouse model

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