Nontuberculous Mycobacteria Prevalence in Bats’ Guano from Caves and Attics of Buildings Studied by Culture and qPCR Examinations

A total of 281 guano samples were collected from caves (N = 181) in eight European countries (Bulgaria, Czech Republic, France, Hungary, Italy, Romania, Slovakia and Slovenia) and attics in the Czech R. (N = 100). The correlation of detection of mycobacteria between Ziehl–Neelsen (ZN) microscopy and...

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Autores principales:	Ivo Pavlik, Vit Ulmann, Helena Modra, Milan Gersl, Barbora Rantova, Jan Zukal, Katerina Zukalova, Ondrej Konecny, Vlastislav Kana, Pavel Kubalek, Vladimir Babak, Ross Tim Weston
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	non-tuberculous mycobacteria (NTM) mycobacteria other than tuberculosis (MOTT) saprophytic environmental mycobacteria risk groups of microorganisms microchiroptera Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f383cc1df9694e8ca4dbc1a96eea48c5
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spelling	oai:doaj.org-article:f383cc1df9694e8ca4dbc1a96eea48c52021-11-25T18:24:27ZNontuberculous Mycobacteria Prevalence in Bats’ Guano from Caves and Attics of Buildings Studied by Culture and qPCR Examinations10.3390/microorganisms91122362076-2607https://doaj.org/article/f383cc1df9694e8ca4dbc1a96eea48c52021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2236https://doaj.org/toc/2076-2607A total of 281 guano samples were collected from caves (N = 181) in eight European countries (Bulgaria, Czech Republic, France, Hungary, Italy, Romania, Slovakia and Slovenia) and attics in the Czech R. (N = 100). The correlation of detection of mycobacteria between Ziehl–Neelsen (ZN) microscopy and culture examination and qPCR was strong. ZN microscopy was positive in guano from caves (58.6%) more than double than positivity in guano from attics (21.0%; <i>p</i> < 0.01). From 89 mycobacterial isolates (73 isolates from cave guano and 16 isolates from attics’ guano), 68 (76.4%) isolates of 19 sp., ssp. and complex were identified as members of three Groups (<i>M. fortuitum</i>, <i>M.</i><i>chelonae</i>, and <i>M. mucogenicum</i>) and four complexes (<i>M. avium</i>, <i>M. terrae</i>, <i>M.</i><i>vaccae</i>, and <i>M.</i><i>smegmatis</i>). A total of 20 isolates (22.5%) belonged to risk group 1 (environmental saprophytes), 48 isolates (53.9%) belonged to risk group 2 (potential pathogens), and none of the isolates belonged to risk group 3 (obligatory pathogens). When comparing bat guano collected from caves and attics, differences (<i>p</i> < 0.01; Mann–Whitney test) were observed for the electrical conductivity, total carbon, total organic, and total inorganic carbon. No difference (<i>p</i> > 0.05; Mann–Whitney test) was found for pH and oxidation-reduction potential parameters.Ivo PavlikVit UlmannHelena ModraMilan GerslBarbora RantovaJan ZukalKaterina ZukalovaOndrej KonecnyVlastislav KanaPavel KubalekVladimir BabakRoss Tim WestonMDPI AGarticlenon-tuberculous mycobacteria (NTM)mycobacteria other than tuberculosis (MOTT)saprophytic environmental mycobacteriarisk groups of microorganismsmicrochiropteraBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2236, p 2236 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	non-tuberculous mycobacteria (NTM) mycobacteria other than tuberculosis (MOTT) saprophytic environmental mycobacteria risk groups of microorganisms microchiroptera Biology (General) QH301-705.5
spellingShingle	non-tuberculous mycobacteria (NTM) mycobacteria other than tuberculosis (MOTT) saprophytic environmental mycobacteria risk groups of microorganisms microchiroptera Biology (General) QH301-705.5 Ivo Pavlik Vit Ulmann Helena Modra Milan Gersl Barbora Rantova Jan Zukal Katerina Zukalova Ondrej Konecny Vlastislav Kana Pavel Kubalek Vladimir Babak Ross Tim Weston Nontuberculous Mycobacteria Prevalence in Bats’ Guano from Caves and Attics of Buildings Studied by Culture and qPCR Examinations
description	A total of 281 guano samples were collected from caves (N = 181) in eight European countries (Bulgaria, Czech Republic, France, Hungary, Italy, Romania, Slovakia and Slovenia) and attics in the Czech R. (N = 100). The correlation of detection of mycobacteria between Ziehl–Neelsen (ZN) microscopy and culture examination and qPCR was strong. ZN microscopy was positive in guano from caves (58.6%) more than double than positivity in guano from attics (21.0%; <i>p</i> < 0.01). From 89 mycobacterial isolates (73 isolates from cave guano and 16 isolates from attics’ guano), 68 (76.4%) isolates of 19 sp., ssp. and complex were identified as members of three Groups (<i>M. fortuitum</i>, <i>M.</i><i>chelonae</i>, and <i>M. mucogenicum</i>) and four complexes (<i>M. avium</i>, <i>M. terrae</i>, <i>M.</i><i>vaccae</i>, and <i>M.</i><i>smegmatis</i>). A total of 20 isolates (22.5%) belonged to risk group 1 (environmental saprophytes), 48 isolates (53.9%) belonged to risk group 2 (potential pathogens), and none of the isolates belonged to risk group 3 (obligatory pathogens). When comparing bat guano collected from caves and attics, differences (<i>p</i> < 0.01; Mann–Whitney test) were observed for the electrical conductivity, total carbon, total organic, and total inorganic carbon. No difference (<i>p</i> > 0.05; Mann–Whitney test) was found for pH and oxidation-reduction potential parameters.
format	article
author	Ivo Pavlik Vit Ulmann Helena Modra Milan Gersl Barbora Rantova Jan Zukal Katerina Zukalova Ondrej Konecny Vlastislav Kana Pavel Kubalek Vladimir Babak Ross Tim Weston
author_facet	Ivo Pavlik Vit Ulmann Helena Modra Milan Gersl Barbora Rantova Jan Zukal Katerina Zukalova Ondrej Konecny Vlastislav Kana Pavel Kubalek Vladimir Babak Ross Tim Weston
author_sort	Ivo Pavlik
title	Nontuberculous Mycobacteria Prevalence in Bats’ Guano from Caves and Attics of Buildings Studied by Culture and qPCR Examinations
title_short	Nontuberculous Mycobacteria Prevalence in Bats’ Guano from Caves and Attics of Buildings Studied by Culture and qPCR Examinations
title_full	Nontuberculous Mycobacteria Prevalence in Bats’ Guano from Caves and Attics of Buildings Studied by Culture and qPCR Examinations
title_fullStr	Nontuberculous Mycobacteria Prevalence in Bats’ Guano from Caves and Attics of Buildings Studied by Culture and qPCR Examinations
title_full_unstemmed	Nontuberculous Mycobacteria Prevalence in Bats’ Guano from Caves and Attics of Buildings Studied by Culture and qPCR Examinations
title_sort	nontuberculous mycobacteria prevalence in bats’ guano from caves and attics of buildings studied by culture and qpcr examinations
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f383cc1df9694e8ca4dbc1a96eea48c5
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Nontuberculous Mycobacteria Prevalence in Bats’ Guano from Caves and Attics of Buildings Studied by Culture and qPCR Examinations

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