Molecular and Morphological Data Confirmed First Record of Abbreviata kazakhstanica Markov and Paraskiv, 1956 (Spirurida: Physalopteridea) in Iran

Background: The genus Abbreviata (Spirurida: Physalopteridea) currently contains 47 species. Physalopteridae nematodes infect a large number of vertebrates, including mammals, birds, reptiles and amphibians. The current study is a report of the first morphological and molecular identification of A....

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Autores principales:	Morteza Ghanbari Johkool, Aida Vafae Eslahi, Milad Badri, Elham Hooshmand, Majid Pirestani, Daniel Jablonski, Rasool Jafari, Abdolhossein Dalimi
Formato:	article
Lenguaje:	EN
Publicado:	Tehran University of Medical Sciences 2021
Materias:	abbreviata kazakhstanica molecular identification pseudopus apodus physalopteridea iran Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/f38760580aa247878461e6c39b071057
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spelling	oai:doaj.org-article:f38760580aa247878461e6c39b0710572021-11-29T07:18:54ZMolecular and Morphological Data Confirmed First Record of Abbreviata kazakhstanica Markov and Paraskiv, 1956 (Spirurida: Physalopteridea) in Iran1735-70202008-238Xhttps://doaj.org/article/f38760580aa247878461e6c39b0710572021-11-01T00:00:00Zhttps://ijpa.tums.ac.ir/index.php/ijpa/article/view/2895https://doaj.org/toc/1735-7020https://doaj.org/toc/2008-238XBackground: The genus Abbreviata (Spirurida: Physalopteridea) currently contains 47 species. Physalopteridae nematodes infect a large number of vertebrates, including mammals, birds, reptiles and amphibians. The current study is a report of the first morphological and molecular identification of A. kazakhstanica (Spirurida: Physalopteridea) in Pseudopus apodus in Iran. Methods: Eleven road-killed P. apodus, were collected from, Iran during 2016-2018. The nematodes were isolated from stomach. After morphological study, the genomic DNA of the parasites was extracted using CTAB method. The DNA was used for PCR amplification of cytochrome c oxidase subunit I (cox1). The PCR products were sequenced, the sequence data were analyzed and multiple alignments were conducted using the Clustal Omega. Results: After detailed microscopic examination, the A. kazakhstanica was identified. The cox1 sequences confirmed the species of helminth. The new sequences of A. kazakhstanica were submitted to GenBank under the accession number MK578751-2. Conclusion: Regarding the limited data on parasitological status of Iranian reptiles, more specific and comprehensive investigations are needed to identify the parasitic fauna.Morteza Ghanbari JohkoolAida Vafae EslahiMilad BadriElham HooshmandMajid PirestaniDaniel JablonskiRasool JafariAbdolhossein DalimiTehran University of Medical Sciencesarticleabbreviata kazakhstanicamolecular identificationpseudopus apodusphysalopterideairanInfectious and parasitic diseasesRC109-216ENIranian Journal of Parasitology, Vol 16, Iss 4, Pp 686-691 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	abbreviata kazakhstanica molecular identification pseudopus apodus physalopteridea iran Infectious and parasitic diseases RC109-216
spellingShingle	abbreviata kazakhstanica molecular identification pseudopus apodus physalopteridea iran Infectious and parasitic diseases RC109-216 Morteza Ghanbari Johkool Aida Vafae Eslahi Milad Badri Elham Hooshmand Majid Pirestani Daniel Jablonski Rasool Jafari Abdolhossein Dalimi Molecular and Morphological Data Confirmed First Record of Abbreviata kazakhstanica Markov and Paraskiv, 1956 (Spirurida: Physalopteridea) in Iran
description	Background: The genus Abbreviata (Spirurida: Physalopteridea) currently contains 47 species. Physalopteridae nematodes infect a large number of vertebrates, including mammals, birds, reptiles and amphibians. The current study is a report of the first morphological and molecular identification of A. kazakhstanica (Spirurida: Physalopteridea) in Pseudopus apodus in Iran. Methods: Eleven road-killed P. apodus, were collected from, Iran during 2016-2018. The nematodes were isolated from stomach. After morphological study, the genomic DNA of the parasites was extracted using CTAB method. The DNA was used for PCR amplification of cytochrome c oxidase subunit I (cox1). The PCR products were sequenced, the sequence data were analyzed and multiple alignments were conducted using the Clustal Omega. Results: After detailed microscopic examination, the A. kazakhstanica was identified. The cox1 sequences confirmed the species of helminth. The new sequences of A. kazakhstanica were submitted to GenBank under the accession number MK578751-2. Conclusion: Regarding the limited data on parasitological status of Iranian reptiles, more specific and comprehensive investigations are needed to identify the parasitic fauna.
format	article
author	Morteza Ghanbari Johkool Aida Vafae Eslahi Milad Badri Elham Hooshmand Majid Pirestani Daniel Jablonski Rasool Jafari Abdolhossein Dalimi
author_facet	Morteza Ghanbari Johkool Aida Vafae Eslahi Milad Badri Elham Hooshmand Majid Pirestani Daniel Jablonski Rasool Jafari Abdolhossein Dalimi
author_sort	Morteza Ghanbari Johkool
title	Molecular and Morphological Data Confirmed First Record of Abbreviata kazakhstanica Markov and Paraskiv, 1956 (Spirurida: Physalopteridea) in Iran
title_short	Molecular and Morphological Data Confirmed First Record of Abbreviata kazakhstanica Markov and Paraskiv, 1956 (Spirurida: Physalopteridea) in Iran
title_full	Molecular and Morphological Data Confirmed First Record of Abbreviata kazakhstanica Markov and Paraskiv, 1956 (Spirurida: Physalopteridea) in Iran
title_fullStr	Molecular and Morphological Data Confirmed First Record of Abbreviata kazakhstanica Markov and Paraskiv, 1956 (Spirurida: Physalopteridea) in Iran
title_full_unstemmed	Molecular and Morphological Data Confirmed First Record of Abbreviata kazakhstanica Markov and Paraskiv, 1956 (Spirurida: Physalopteridea) in Iran
title_sort	molecular and morphological data confirmed first record of abbreviata kazakhstanica markov and paraskiv, 1956 (spirurida: physalopteridea) in iran
publisher	Tehran University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/f38760580aa247878461e6c39b071057
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Molecular and Morphological Data Confirmed First Record of Abbreviata kazakhstanica Markov and Paraskiv, 1956 (Spirurida: Physalopteridea) in Iran

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