Specific IgG response against Mycobacterium avium paratuberculosis in children and adults with Crohn's disease.

<h4>Background and aims</h4>Presence of serum antibodies against Mycobacterium avium paratuberculosis (MAP) in Crohn's Disease (CD) as a disease characteristic remains controversial. In the present work, we assessed antibody reactivity of serum and intestinal fluid against four dist...

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Autores principales:	Julien Verdier, Louis Deroche, Matthieu Allez, Caroline Loy, Franck Biet, Christelle C Bodier, Sylvie Bay, Christelle Ganneau, Tamara Matysiak-Budnik, Jean Marc Reyrat, Martine Heyman, Nadine Cerf-Bensussan, Frank M Ruemmele, Sandrine Ménard
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f38a9c33d5ab418297616d5004233094
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spelling	oai:doaj.org-article:f38a9c33d5ab418297616d50042330942021-11-18T07:47:02ZSpecific IgG response against Mycobacterium avium paratuberculosis in children and adults with Crohn's disease.1932-620310.1371/journal.pone.0062780https://doaj.org/article/f38a9c33d5ab418297616d50042330942013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23658774/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background and aims</h4>Presence of serum antibodies against Mycobacterium avium paratuberculosis (MAP) in Crohn's Disease (CD) as a disease characteristic remains controversial. In the present work, we assessed antibody reactivity of serum and intestinal fluid against four distinct MAP-antigens, including the recently identified MAP-specific lipopentapeptide (L5P).<h4>Methods</h4>Immunoglobulin concentrations and specificity against 3 non MAP-specific antigens: glycosyl-transferase-d (GSD), purified protein derivative from MAP (Johnin-PPD), heparin binding haemagglutinin (MAP-HBHA) and one MAP-specific antigen: synthetic L5P were determined by ELISA in gut lavage fluids from adult controls or patients with CD, and in sera of children or adult controls or patients with CD, ulcerative colitis or celiac disease.<h4>Results</h4>Total IgA and IgG concentrations were increased in sera of children with CD but were decreased in sera of adults with CD, thereof specificity against MAP antigens was assessed by normalizing immunoglobulin concentrations between samples. In CD patients, IgG reactivity was increased against the four MAP antigens, including L5P in gut lavage fluids but it was only increased against L5P in sera. By contrast, anti-L5P IgG were not increased in patients with ulcerative colitis or celiac disease.<h4>Conclusions</h4>A significant increase in anti-L5P IgG is observed in sera of children and adults with CD but not in patients with other intestinal inflammatory diseases. Anti-L5P antibodies may serve as serological marker for CD.Julien VerdierLouis DerocheMatthieu AllezCaroline LoyFranck BietChristelle C BodierSylvie BayChristelle GanneauTamara Matysiak-BudnikJean Marc ReyratMartine HeymanNadine Cerf-BensussanFrank M RuemmeleSandrine MénardPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e62780 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Julien Verdier Louis Deroche Matthieu Allez Caroline Loy Franck Biet Christelle C Bodier Sylvie Bay Christelle Ganneau Tamara Matysiak-Budnik Jean Marc Reyrat Martine Heyman Nadine Cerf-Bensussan Frank M Ruemmele Sandrine Ménard Specific IgG response against Mycobacterium avium paratuberculosis in children and adults with Crohn's disease.
description	<h4>Background and aims</h4>Presence of serum antibodies against Mycobacterium avium paratuberculosis (MAP) in Crohn's Disease (CD) as a disease characteristic remains controversial. In the present work, we assessed antibody reactivity of serum and intestinal fluid against four distinct MAP-antigens, including the recently identified MAP-specific lipopentapeptide (L5P).<h4>Methods</h4>Immunoglobulin concentrations and specificity against 3 non MAP-specific antigens: glycosyl-transferase-d (GSD), purified protein derivative from MAP (Johnin-PPD), heparin binding haemagglutinin (MAP-HBHA) and one MAP-specific antigen: synthetic L5P were determined by ELISA in gut lavage fluids from adult controls or patients with CD, and in sera of children or adult controls or patients with CD, ulcerative colitis or celiac disease.<h4>Results</h4>Total IgA and IgG concentrations were increased in sera of children with CD but were decreased in sera of adults with CD, thereof specificity against MAP antigens was assessed by normalizing immunoglobulin concentrations between samples. In CD patients, IgG reactivity was increased against the four MAP antigens, including L5P in gut lavage fluids but it was only increased against L5P in sera. By contrast, anti-L5P IgG were not increased in patients with ulcerative colitis or celiac disease.<h4>Conclusions</h4>A significant increase in anti-L5P IgG is observed in sera of children and adults with CD but not in patients with other intestinal inflammatory diseases. Anti-L5P antibodies may serve as serological marker for CD.
format	article
author	Julien Verdier Louis Deroche Matthieu Allez Caroline Loy Franck Biet Christelle C Bodier Sylvie Bay Christelle Ganneau Tamara Matysiak-Budnik Jean Marc Reyrat Martine Heyman Nadine Cerf-Bensussan Frank M Ruemmele Sandrine Ménard
author_facet	Julien Verdier Louis Deroche Matthieu Allez Caroline Loy Franck Biet Christelle C Bodier Sylvie Bay Christelle Ganneau Tamara Matysiak-Budnik Jean Marc Reyrat Martine Heyman Nadine Cerf-Bensussan Frank M Ruemmele Sandrine Ménard
author_sort	Julien Verdier
title	Specific IgG response against Mycobacterium avium paratuberculosis in children and adults with Crohn's disease.
title_short	Specific IgG response against Mycobacterium avium paratuberculosis in children and adults with Crohn's disease.
title_full	Specific IgG response against Mycobacterium avium paratuberculosis in children and adults with Crohn's disease.
title_fullStr	Specific IgG response against Mycobacterium avium paratuberculosis in children and adults with Crohn's disease.
title_full_unstemmed	Specific IgG response against Mycobacterium avium paratuberculosis in children and adults with Crohn's disease.
title_sort	specific igg response against mycobacterium avium paratuberculosis in children and adults with crohn's disease.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/f38a9c33d5ab418297616d5004233094
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Specific IgG response against Mycobacterium avium paratuberculosis in children and adults with Crohn's disease.

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