The transcriptome analysis on urea response mechanism in the process of ergosterol synthesis by Cordyceps cicadae

Abstract Nitrogen source is required for the growth of Cordyceps cicadae and involved in the regulation of metabolite synthesis. In order to further investigate the regulatory effects of nitrogen sources on the ergosterol synthesis by C. cicadae. We first confirmed that urea could significantly incr...

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Autores principales:	Qihui Su, Zhicai Zhang, Xiaocui Liu, Feng Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f39a1c1defe84c4e9ac5baa79ae574e9
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spelling	oai:doaj.org-article:f39a1c1defe84c4e9ac5baa79ae574e92021-12-02T16:53:02ZThe transcriptome analysis on urea response mechanism in the process of ergosterol synthesis by Cordyceps cicadae10.1038/s41598-021-90377-22045-2322https://doaj.org/article/f39a1c1defe84c4e9ac5baa79ae574e92021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90377-2https://doaj.org/toc/2045-2322Abstract Nitrogen source is required for the growth of Cordyceps cicadae and involved in the regulation of metabolite synthesis. In order to further investigate the regulatory effects of nitrogen sources on the ergosterol synthesis by C. cicadae. We first confirmed that urea could significantly increase the ergosterol synthesis. The transcriptome analysis showed that compared with biomass cultured in the control fermentation medium (CFM), 1340 differentially expressed genes (DEGs) were obtained by Gene Ontology (GO) annotation, and 312 DEGs were obtained by Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation from the biomass cultured in CFM + CO(NH2)2. Urea up-regulated D-3-phosphoglycerate dehydrogenase gene transcription level and down-regulated enolase and L-serine/L-threonine ammonialyase gene transcription level, increased serine synthesis, allosterically activate pyruvate kinase, to promote the synthesis of pyruvate and CH3CO ~ SCOA, the primer of ergosterol; Urea increase the genes transcription related with ergosterol synthesis by up-regulating the steroid regulatory element binding protein gene transcription levels. The transcriptome results were provided by those of qRT-PCR. Collectively, our finding provided valuable insights into the regulatory effect of nitrogen source on the ergosterol synthesis by C. cicadae.Qihui SuZhicai ZhangXiaocui LiuFeng WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qihui Su Zhicai Zhang Xiaocui Liu Feng Wang The transcriptome analysis on urea response mechanism in the process of ergosterol synthesis by Cordyceps cicadae
description	Abstract Nitrogen source is required for the growth of Cordyceps cicadae and involved in the regulation of metabolite synthesis. In order to further investigate the regulatory effects of nitrogen sources on the ergosterol synthesis by C. cicadae. We first confirmed that urea could significantly increase the ergosterol synthesis. The transcriptome analysis showed that compared with biomass cultured in the control fermentation medium (CFM), 1340 differentially expressed genes (DEGs) were obtained by Gene Ontology (GO) annotation, and 312 DEGs were obtained by Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation from the biomass cultured in CFM + CO(NH2)2. Urea up-regulated D-3-phosphoglycerate dehydrogenase gene transcription level and down-regulated enolase and L-serine/L-threonine ammonialyase gene transcription level, increased serine synthesis, allosterically activate pyruvate kinase, to promote the synthesis of pyruvate and CH3CO ~ SCOA, the primer of ergosterol; Urea increase the genes transcription related with ergosterol synthesis by up-regulating the steroid regulatory element binding protein gene transcription levels. The transcriptome results were provided by those of qRT-PCR. Collectively, our finding provided valuable insights into the regulatory effect of nitrogen source on the ergosterol synthesis by C. cicadae.
format	article
author	Qihui Su Zhicai Zhang Xiaocui Liu Feng Wang
author_facet	Qihui Su Zhicai Zhang Xiaocui Liu Feng Wang
author_sort	Qihui Su
title	The transcriptome analysis on urea response mechanism in the process of ergosterol synthesis by Cordyceps cicadae
title_short	The transcriptome analysis on urea response mechanism in the process of ergosterol synthesis by Cordyceps cicadae
title_full	The transcriptome analysis on urea response mechanism in the process of ergosterol synthesis by Cordyceps cicadae
title_fullStr	The transcriptome analysis on urea response mechanism in the process of ergosterol synthesis by Cordyceps cicadae
title_full_unstemmed	The transcriptome analysis on urea response mechanism in the process of ergosterol synthesis by Cordyceps cicadae
title_sort	transcriptome analysis on urea response mechanism in the process of ergosterol synthesis by cordyceps cicadae
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f39a1c1defe84c4e9ac5baa79ae574e9
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The transcriptome analysis on urea response mechanism in the process of ergosterol synthesis by Cordyceps cicadae

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