Exploring the nasopharyngeal microbiota composition in infants with whooping cough: A test-negative case-control study

<h4>Purpose</h4> The purpose of this study was to characterize the nasopharyngeal microbiota of infants with possible and confirmed pertussis compared to healthy controls. <h4>Methods</h4> This prospective study included all infants <1 year with microbiologically confirmed...

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Autores principales:	Muntsa Rocafort, Desiree Henares, Pedro Brotons, Irene Barrabeig, Cristian Launes, Lore Merdrignac, Marta Valenciano, Angela Domínguez, Pere Godoy, Carmen Muñoz-Almagro
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f39a40c1fefe4973883f5f65c36060ed
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spelling	oai:doaj.org-article:f39a40c1fefe4973883f5f65c36060ed2021-11-04T07:42:10ZExploring the nasopharyngeal microbiota composition in infants with whooping cough: A test-negative case-control study1932-6203https://doaj.org/article/f39a40c1fefe4973883f5f65c36060ed2021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8555781/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Purpose</h4> The purpose of this study was to characterize the nasopharyngeal microbiota of infants with possible and confirmed pertussis compared to healthy controls. <h4>Methods</h4> This prospective study included all infants <1 year with microbiologically confirmed diagnosis of pertussis attended at a University Hospital over a 12-month period. For each confirmed case, up to 2 consecutive patients within the same age range and meeting the clinical case definition of pertussis but testing PCR-negative were included as possible cases. A third group of asymptomatic infants (healthy controls) were also included. Nasopharyngeal microbiota was characterized by sequencing the V3-V4 region of the 16S rRNA gene. Common respiratory DNA/RNA viral co-infection was tested by multiplex PCR. <h4>Results</h4> Twelve confirmed cases, 21 possible cases and 9 healthy controls were included. Confirmed whooping cough was primarily driven by detection of Bordetella with no other major changes on nasopharyngeal microbiota. Possible cases had limited abundance or absence of Bordetella and a distinctive microbiota with lower bacterial richness and diversity and higher rates of viral co-infection than both confirmed cases and healthy controls. Bordetella reads determined by 16S rRNA gene sequencing were found in all 12 confirmed cases (100%), 3 out of the 21 possible cases (14.3%) but in any healthy control. <h4>Conclusion</h4> This study supports the usefulness of 16S rRNA gene sequencing for improved sensitivity on pertussis diagnosis compared to real-time PCR and to understand other microbial changes occurring in the nasopharynx in children <1 year old with suspected whooping cough compared to healthy controls.Muntsa RocafortDesiree HenaresPedro BrotonsIrene BarrabeigCristian LaunesLore MerdrignacMarta ValencianoAngela DomínguezPere GodoyCarmen Muñoz-AlmagroPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Muntsa Rocafort Desiree Henares Pedro Brotons Irene Barrabeig Cristian Launes Lore Merdrignac Marta Valenciano Angela Domínguez Pere Godoy Carmen Muñoz-Almagro Exploring the nasopharyngeal microbiota composition in infants with whooping cough: A test-negative case-control study
description	<h4>Purpose</h4> The purpose of this study was to characterize the nasopharyngeal microbiota of infants with possible and confirmed pertussis compared to healthy controls. <h4>Methods</h4> This prospective study included all infants <1 year with microbiologically confirmed diagnosis of pertussis attended at a University Hospital over a 12-month period. For each confirmed case, up to 2 consecutive patients within the same age range and meeting the clinical case definition of pertussis but testing PCR-negative were included as possible cases. A third group of asymptomatic infants (healthy controls) were also included. Nasopharyngeal microbiota was characterized by sequencing the V3-V4 region of the 16S rRNA gene. Common respiratory DNA/RNA viral co-infection was tested by multiplex PCR. <h4>Results</h4> Twelve confirmed cases, 21 possible cases and 9 healthy controls were included. Confirmed whooping cough was primarily driven by detection of Bordetella with no other major changes on nasopharyngeal microbiota. Possible cases had limited abundance or absence of Bordetella and a distinctive microbiota with lower bacterial richness and diversity and higher rates of viral co-infection than both confirmed cases and healthy controls. Bordetella reads determined by 16S rRNA gene sequencing were found in all 12 confirmed cases (100%), 3 out of the 21 possible cases (14.3%) but in any healthy control. <h4>Conclusion</h4> This study supports the usefulness of 16S rRNA gene sequencing for improved sensitivity on pertussis diagnosis compared to real-time PCR and to understand other microbial changes occurring in the nasopharynx in children <1 year old with suspected whooping cough compared to healthy controls.
format	article
author	Muntsa Rocafort Desiree Henares Pedro Brotons Irene Barrabeig Cristian Launes Lore Merdrignac Marta Valenciano Angela Domínguez Pere Godoy Carmen Muñoz-Almagro
author_facet	Muntsa Rocafort Desiree Henares Pedro Brotons Irene Barrabeig Cristian Launes Lore Merdrignac Marta Valenciano Angela Domínguez Pere Godoy Carmen Muñoz-Almagro
author_sort	Muntsa Rocafort
title	Exploring the nasopharyngeal microbiota composition in infants with whooping cough: A test-negative case-control study
title_short	Exploring the nasopharyngeal microbiota composition in infants with whooping cough: A test-negative case-control study
title_full	Exploring the nasopharyngeal microbiota composition in infants with whooping cough: A test-negative case-control study
title_fullStr	Exploring the nasopharyngeal microbiota composition in infants with whooping cough: A test-negative case-control study
title_full_unstemmed	Exploring the nasopharyngeal microbiota composition in infants with whooping cough: A test-negative case-control study
title_sort	exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control study
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f39a40c1fefe4973883f5f65c36060ed
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Exploring the nasopharyngeal microbiota composition in infants with whooping cough: A test-negative case-control study

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