Hendra and Nipah Virus Infection in Cultured Human Olfactory Epithelial Cells

ABSTRACT Henipaviruses are emerging zoonotic viruses and causative agents of encephalitis in humans. However, the mechanisms of entry into the central nervous system (CNS) in humans are not known. Here, we evaluated the possible role of olfactory epithelium in virus entry into the CNS. We characteri...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Viktoriya Borisevich, Mehmet Hakan Ozdener, Bilal Malik, Barry Rockx
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	Henipavirus neuroinvasion olfactory epithelium Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f3a1648980274c38a7f100e653088cdc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f3a1648980274c38a7f100e653088cdc
record_format	dspace
spelling	oai:doaj.org-article:f3a1648980274c38a7f100e653088cdc2021-11-15T15:21:47ZHendra and Nipah Virus Infection in Cultured Human Olfactory Epithelial Cells10.1128/mSphere.00252-172379-5042https://doaj.org/article/f3a1648980274c38a7f100e653088cdc2017-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00252-17https://doaj.org/toc/2379-5042ABSTRACT Henipaviruses are emerging zoonotic viruses and causative agents of encephalitis in humans. However, the mechanisms of entry into the central nervous system (CNS) in humans are not known. Here, we evaluated the possible role of olfactory epithelium in virus entry into the CNS. We characterized Hendra virus (HeV) and Nipah virus (NiV) infection of primary human olfactory epithelial cultures. We show that henipaviruses can infect mature olfactory sensory neurons. Henipaviruses replicated efficiently, resulting in cytopathic effect and limited induction of host responses. These results show that human olfactory epithelium is susceptible to infection with henipaviruses, suggesting that this could be a pathway for neuroinvasion in humans. IMPORTANCE Henipaviruses are emerging zoonotic pathogens that can cause acute and severe respiratory and neurological disease in humans. The pathways by which henipaviruses enter the central nervous system (CNS) in humans are still unknown. The observation that human olfactory neurons are highly susceptible to infection with henipaviruses demonstrates that the olfactory epithelium can serve as a site of Henipavirus entry into the CNS.Viktoriya BorisevichMehmet Hakan OzdenerBilal MalikBarry RockxAmerican Society for MicrobiologyarticleHenipavirusneuroinvasionolfactory epitheliumMicrobiologyQR1-502ENmSphere, Vol 2, Iss 3 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Henipavirus neuroinvasion olfactory epithelium Microbiology QR1-502
spellingShingle	Henipavirus neuroinvasion olfactory epithelium Microbiology QR1-502 Viktoriya Borisevich Mehmet Hakan Ozdener Bilal Malik Barry Rockx Hendra and Nipah Virus Infection in Cultured Human Olfactory Epithelial Cells
description	ABSTRACT Henipaviruses are emerging zoonotic viruses and causative agents of encephalitis in humans. However, the mechanisms of entry into the central nervous system (CNS) in humans are not known. Here, we evaluated the possible role of olfactory epithelium in virus entry into the CNS. We characterized Hendra virus (HeV) and Nipah virus (NiV) infection of primary human olfactory epithelial cultures. We show that henipaviruses can infect mature olfactory sensory neurons. Henipaviruses replicated efficiently, resulting in cytopathic effect and limited induction of host responses. These results show that human olfactory epithelium is susceptible to infection with henipaviruses, suggesting that this could be a pathway for neuroinvasion in humans. IMPORTANCE Henipaviruses are emerging zoonotic pathogens that can cause acute and severe respiratory and neurological disease in humans. The pathways by which henipaviruses enter the central nervous system (CNS) in humans are still unknown. The observation that human olfactory neurons are highly susceptible to infection with henipaviruses demonstrates that the olfactory epithelium can serve as a site of Henipavirus entry into the CNS.
format	article
author	Viktoriya Borisevich Mehmet Hakan Ozdener Bilal Malik Barry Rockx
author_facet	Viktoriya Borisevich Mehmet Hakan Ozdener Bilal Malik Barry Rockx
author_sort	Viktoriya Borisevich
title	Hendra and Nipah Virus Infection in Cultured Human Olfactory Epithelial Cells
title_short	Hendra and Nipah Virus Infection in Cultured Human Olfactory Epithelial Cells
title_full	Hendra and Nipah Virus Infection in Cultured Human Olfactory Epithelial Cells
title_fullStr	Hendra and Nipah Virus Infection in Cultured Human Olfactory Epithelial Cells
title_full_unstemmed	Hendra and Nipah Virus Infection in Cultured Human Olfactory Epithelial Cells
title_sort	hendra and nipah virus infection in cultured human olfactory epithelial cells
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/f3a1648980274c38a7f100e653088cdc
work_keys_str_mv	AT viktoriyaborisevich hendraandnipahvirusinfectioninculturedhumanolfactoryepithelialcells AT mehmethakanozdener hendraandnipahvirusinfectioninculturedhumanolfactoryepithelialcells AT bilalmalik hendraandnipahvirusinfectioninculturedhumanolfactoryepithelialcells AT barryrockx hendraandnipahvirusinfectioninculturedhumanolfactoryepithelialcells
_version_	1718428167575699456

Hendra and Nipah Virus Infection in Cultured Human Olfactory Epithelial Cells

Ejemplares similares