NOF1 encodes an Arabidopsis protein involved in the control of rRNA expression.

The control of ribosomal RNA biogenesis is essential for the regulation of protein synthesis in eukaryotic cells. Here, we report the characterization of NOF1 that encodes a putative nucleolar protein involved in the control of rRNA expression in Arabidopsis. The gene has been isolated by T-DNA tagg...

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Autores principales:	Erwana Harscoët, Bertrand Dubreucq, Jean-Christophe Palauqui, Loïc Lepiniec
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f3a5e73316d84bd6ad5c3be8e3a2abd8
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spelling	oai:doaj.org-article:f3a5e73316d84bd6ad5c3be8e3a2abd82021-11-18T06:34:59ZNOF1 encodes an Arabidopsis protein involved in the control of rRNA expression.1932-620310.1371/journal.pone.0012829https://doaj.org/article/f3a5e73316d84bd6ad5c3be8e3a2abd82010-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20877469/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The control of ribosomal RNA biogenesis is essential for the regulation of protein synthesis in eukaryotic cells. Here, we report the characterization of NOF1 that encodes a putative nucleolar protein involved in the control of rRNA expression in Arabidopsis. The gene has been isolated by T-DNA tagging and its function verified by the characterization of a second allele and genetic complementation of the mutants. The nof1 mutants are affected in female gametogenesis and embryo development. This result is consistent with the detection of NOF1 mRNA in all tissues throughout plant life's cycle, and preferentially in differentiating cells. Interestingly, the closely related proteins from zebra fish and yeast are also necessary for cell division and differentiation. We showed that the nof1-1 mutant displays higher rRNA expression and hypomethylation of rRNA promoter. Taken together, the results presented here demonstrated that NOF1 is an Arabidopsis gene involved in the control of rRNA expression, and suggested that it encodes a putative nucleolar protein, the function of which may be conserved in eukaryotes.Erwana HarscoëtBertrand DubreucqJean-Christophe PalauquiLoïc LepiniecPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 9, p e12829 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Erwana Harscoët Bertrand Dubreucq Jean-Christophe Palauqui Loïc Lepiniec NOF1 encodes an Arabidopsis protein involved in the control of rRNA expression.
description	The control of ribosomal RNA biogenesis is essential for the regulation of protein synthesis in eukaryotic cells. Here, we report the characterization of NOF1 that encodes a putative nucleolar protein involved in the control of rRNA expression in Arabidopsis. The gene has been isolated by T-DNA tagging and its function verified by the characterization of a second allele and genetic complementation of the mutants. The nof1 mutants are affected in female gametogenesis and embryo development. This result is consistent with the detection of NOF1 mRNA in all tissues throughout plant life's cycle, and preferentially in differentiating cells. Interestingly, the closely related proteins from zebra fish and yeast are also necessary for cell division and differentiation. We showed that the nof1-1 mutant displays higher rRNA expression and hypomethylation of rRNA promoter. Taken together, the results presented here demonstrated that NOF1 is an Arabidopsis gene involved in the control of rRNA expression, and suggested that it encodes a putative nucleolar protein, the function of which may be conserved in eukaryotes.
format	article
author	Erwana Harscoët Bertrand Dubreucq Jean-Christophe Palauqui Loïc Lepiniec
author_facet	Erwana Harscoët Bertrand Dubreucq Jean-Christophe Palauqui Loïc Lepiniec
author_sort	Erwana Harscoët
title	NOF1 encodes an Arabidopsis protein involved in the control of rRNA expression.
title_short	NOF1 encodes an Arabidopsis protein involved in the control of rRNA expression.
title_full	NOF1 encodes an Arabidopsis protein involved in the control of rRNA expression.
title_fullStr	NOF1 encodes an Arabidopsis protein involved in the control of rRNA expression.
title_full_unstemmed	NOF1 encodes an Arabidopsis protein involved in the control of rRNA expression.
title_sort	nof1 encodes an arabidopsis protein involved in the control of rrna expression.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/f3a5e73316d84bd6ad5c3be8e3a2abd8
work_keys_str_mv	AT erwanaharscoet nof1encodesanarabidopsisproteininvolvedinthecontrolofrrnaexpression AT bertranddubreucq nof1encodesanarabidopsisproteininvolvedinthecontrolofrrnaexpression AT jeanchristophepalauqui nof1encodesanarabidopsisproteininvolvedinthecontrolofrrnaexpression AT loiclepiniec nof1encodesanarabidopsisproteininvolvedinthecontrolofrrnaexpression
_version_	1718424515350888448

NOF1 encodes an Arabidopsis protein involved in the control of rRNA expression.

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