Effects of atrial natriuretic peptide on bicarbonate transport in long- and short-looped medullary thick ascending limbs of rats.

Atrial natriuretic peptide (ANP) is known to influence NaCl transport in the medullary thick ascending limbs (MAL), where the largest NaCl reabsorption occurs among distal nephron segments in response to arginine vasopressin (AVP). In the present study, we investigated the effect of ANP on bicarbona...

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Autores principales:	Hiroshi Nonoguchi, Yuichiro Izumi, Yushi Nakayama, Takanobu Matsuzaki, Yukiko Yasuoka, Takeaki Inoue, Hideki Inoue, Tomohiko Mouri, Katsumasa Kawahara, Hideyuki Saito, Kimio Tomita
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f3aa19cdced24004a64f30917646aa0a
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spelling	oai:doaj.org-article:f3aa19cdced24004a64f30917646aa0a2021-11-18T08:40:38ZEffects of atrial natriuretic peptide on bicarbonate transport in long- and short-looped medullary thick ascending limbs of rats.1932-620310.1371/journal.pone.0083146https://doaj.org/article/f3aa19cdced24004a64f30917646aa0a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24376658/?tool=EBIhttps://doaj.org/toc/1932-6203Atrial natriuretic peptide (ANP) is known to influence NaCl transport in the medullary thick ascending limbs (MAL), where the largest NaCl reabsorption occurs among distal nephron segments in response to arginine vasopressin (AVP). In the present study, we investigated the effect of ANP on bicarbonate (HCO3 (-)) transport in the MAL using an isolated tubule perfusion technique. The HCO3 (-) concentration was measured using free-flow ultramicro-fluorometer. We first observed basal HCO3 (-) reabsorption in both long- and short-looped MALs (lMALs, and sMALs, respectively). AVP inhibited HCO3 (-) reabsorption in both lMALs and sMALs, whereas ANP did not change HCO3 (-) transport. However, in the presence of AVP, ANP restored the HCO3 (-) reabsorption inhibited by AVP both in lMAL and sMAL. The effects of ANP on HCO3 (-) transport was mimicked by cyclic GMP. The mRNA expression level of the vasopressin V2 receptor in lMALs was significantly higher than in sMALs, whereas expression of the V1a receptor was unchanged. In summary, AVP inhibits HCO3 (-) transport, and ANP counteracts the action of AVP on HCO3 (-) transport both in lMALs and sMALs.Hiroshi NonoguchiYuichiro IzumiYushi NakayamaTakanobu MatsuzakiYukiko YasuokaTakeaki InoueHideki InoueTomohiko MouriKatsumasa KawaharaHideyuki SaitoKimio TomitaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e83146 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hiroshi Nonoguchi Yuichiro Izumi Yushi Nakayama Takanobu Matsuzaki Yukiko Yasuoka Takeaki Inoue Hideki Inoue Tomohiko Mouri Katsumasa Kawahara Hideyuki Saito Kimio Tomita Effects of atrial natriuretic peptide on bicarbonate transport in long- and short-looped medullary thick ascending limbs of rats.
description	Atrial natriuretic peptide (ANP) is known to influence NaCl transport in the medullary thick ascending limbs (MAL), where the largest NaCl reabsorption occurs among distal nephron segments in response to arginine vasopressin (AVP). In the present study, we investigated the effect of ANP on bicarbonate (HCO3 (-)) transport in the MAL using an isolated tubule perfusion technique. The HCO3 (-) concentration was measured using free-flow ultramicro-fluorometer. We first observed basal HCO3 (-) reabsorption in both long- and short-looped MALs (lMALs, and sMALs, respectively). AVP inhibited HCO3 (-) reabsorption in both lMALs and sMALs, whereas ANP did not change HCO3 (-) transport. However, in the presence of AVP, ANP restored the HCO3 (-) reabsorption inhibited by AVP both in lMAL and sMAL. The effects of ANP on HCO3 (-) transport was mimicked by cyclic GMP. The mRNA expression level of the vasopressin V2 receptor in lMALs was significantly higher than in sMALs, whereas expression of the V1a receptor was unchanged. In summary, AVP inhibits HCO3 (-) transport, and ANP counteracts the action of AVP on HCO3 (-) transport both in lMALs and sMALs.
format	article
author	Hiroshi Nonoguchi Yuichiro Izumi Yushi Nakayama Takanobu Matsuzaki Yukiko Yasuoka Takeaki Inoue Hideki Inoue Tomohiko Mouri Katsumasa Kawahara Hideyuki Saito Kimio Tomita
author_facet	Hiroshi Nonoguchi Yuichiro Izumi Yushi Nakayama Takanobu Matsuzaki Yukiko Yasuoka Takeaki Inoue Hideki Inoue Tomohiko Mouri Katsumasa Kawahara Hideyuki Saito Kimio Tomita
author_sort	Hiroshi Nonoguchi
title	Effects of atrial natriuretic peptide on bicarbonate transport in long- and short-looped medullary thick ascending limbs of rats.
title_short	Effects of atrial natriuretic peptide on bicarbonate transport in long- and short-looped medullary thick ascending limbs of rats.
title_full	Effects of atrial natriuretic peptide on bicarbonate transport in long- and short-looped medullary thick ascending limbs of rats.
title_fullStr	Effects of atrial natriuretic peptide on bicarbonate transport in long- and short-looped medullary thick ascending limbs of rats.
title_full_unstemmed	Effects of atrial natriuretic peptide on bicarbonate transport in long- and short-looped medullary thick ascending limbs of rats.
title_sort	effects of atrial natriuretic peptide on bicarbonate transport in long- and short-looped medullary thick ascending limbs of rats.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/f3aa19cdced24004a64f30917646aa0a
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Effects of atrial natriuretic peptide on bicarbonate transport in long- and short-looped medullary thick ascending limbs of rats.

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