Prophage-Dependent Neighbor Predation Fosters Horizontal Gene Transfer by Natural Transformation

ABSTRACT Natural transformation is a broadly conserved mechanism of horizontal gene transfer (HGT) in bacteria that can shape their evolution through the acquisition of genes that promote virulence, antibiotic resistance, and other traits. Recent work has established that neighbor predation via type...

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Autores principales:	Roberto C. Molina-Quiroz, Triana N. Dalia, Andrew Camilli, Ankur B. Dalia, Cecilia A. Silva-Valenzuela
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	HGT Vibrio cholerae bacteriophages chitin natural transformation neighbor predation Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f3adafb05b0b4fa78a51d6e7bd7819aa
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spelling	oai:doaj.org-article:f3adafb05b0b4fa78a51d6e7bd7819aa2021-11-15T15:31:13ZProphage-Dependent Neighbor Predation Fosters Horizontal Gene Transfer by Natural Transformation10.1128/mSphere.00975-202379-5042https://doaj.org/article/f3adafb05b0b4fa78a51d6e7bd7819aa2020-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00975-20https://doaj.org/toc/2379-5042ABSTRACT Natural transformation is a broadly conserved mechanism of horizontal gene transfer (HGT) in bacteria that can shape their evolution through the acquisition of genes that promote virulence, antibiotic resistance, and other traits. Recent work has established that neighbor predation via type VI secretion systems, bacteriocins, and virulent phages plays an important role in promoting HGT. Here, we demonstrate that in chitin estuary microcosms, Vibrio cholerae K139 lysogens exhibit prophage-dependent neighbor predation of nonlysogens to enhance HGT. Through predation of nonlysogens, K139 lysogens also have a fitness advantage under these microcosm conditions. The ecological strategy revealed by our work provides a better understanding of the evolutionary mechanisms used by bacteria to adapt in their natural setting and contributes to our understanding of the selective pressures that may drive prophage maintenance in bacterial genomes. IMPORTANCE Prophages are nearly ubiquitous in bacterial species. These integrated phage elements have previously been implicated in horizontal gene transfer (HGT) largely through their ability to carry out transduction (generalized or specialized). Here, we show that prophage-encoded viral particles promote neighbor predation leading to enhanced HGT by natural transformation in the waterborne pathogen Vibrio cholerae. Our findings contribute to a comprehensive understanding of the dynamic forces involved in prophage maintenance which ultimately drive the evolution of naturally competent bacteria in their natural environment.Roberto C. Molina-QuirozTriana N. DaliaAndrew CamilliAnkur B. DaliaCecilia A. Silva-ValenzuelaAmerican Society for MicrobiologyarticleHGTVibrio choleraebacteriophageschitinnatural transformationneighbor predationMicrobiologyQR1-502ENmSphere, Vol 5, Iss 6 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	HGT Vibrio cholerae bacteriophages chitin natural transformation neighbor predation Microbiology QR1-502
spellingShingle	HGT Vibrio cholerae bacteriophages chitin natural transformation neighbor predation Microbiology QR1-502 Roberto C. Molina-Quiroz Triana N. Dalia Andrew Camilli Ankur B. Dalia Cecilia A. Silva-Valenzuela Prophage-Dependent Neighbor Predation Fosters Horizontal Gene Transfer by Natural Transformation
description	ABSTRACT Natural transformation is a broadly conserved mechanism of horizontal gene transfer (HGT) in bacteria that can shape their evolution through the acquisition of genes that promote virulence, antibiotic resistance, and other traits. Recent work has established that neighbor predation via type VI secretion systems, bacteriocins, and virulent phages plays an important role in promoting HGT. Here, we demonstrate that in chitin estuary microcosms, Vibrio cholerae K139 lysogens exhibit prophage-dependent neighbor predation of nonlysogens to enhance HGT. Through predation of nonlysogens, K139 lysogens also have a fitness advantage under these microcosm conditions. The ecological strategy revealed by our work provides a better understanding of the evolutionary mechanisms used by bacteria to adapt in their natural setting and contributes to our understanding of the selective pressures that may drive prophage maintenance in bacterial genomes. IMPORTANCE Prophages are nearly ubiquitous in bacterial species. These integrated phage elements have previously been implicated in horizontal gene transfer (HGT) largely through their ability to carry out transduction (generalized or specialized). Here, we show that prophage-encoded viral particles promote neighbor predation leading to enhanced HGT by natural transformation in the waterborne pathogen Vibrio cholerae. Our findings contribute to a comprehensive understanding of the dynamic forces involved in prophage maintenance which ultimately drive the evolution of naturally competent bacteria in their natural environment.
format	article
author	Roberto C. Molina-Quiroz Triana N. Dalia Andrew Camilli Ankur B. Dalia Cecilia A. Silva-Valenzuela
author_facet	Roberto C. Molina-Quiroz Triana N. Dalia Andrew Camilli Ankur B. Dalia Cecilia A. Silva-Valenzuela
author_sort	Roberto C. Molina-Quiroz
title	Prophage-Dependent Neighbor Predation Fosters Horizontal Gene Transfer by Natural Transformation
title_short	Prophage-Dependent Neighbor Predation Fosters Horizontal Gene Transfer by Natural Transformation
title_full	Prophage-Dependent Neighbor Predation Fosters Horizontal Gene Transfer by Natural Transformation
title_fullStr	Prophage-Dependent Neighbor Predation Fosters Horizontal Gene Transfer by Natural Transformation
title_full_unstemmed	Prophage-Dependent Neighbor Predation Fosters Horizontal Gene Transfer by Natural Transformation
title_sort	prophage-dependent neighbor predation fosters horizontal gene transfer by natural transformation
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/f3adafb05b0b4fa78a51d6e7bd7819aa
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Prophage-Dependent Neighbor Predation Fosters Horizontal Gene Transfer by Natural Transformation

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