RNA-seq analysis of host and viral gene expression highlights interaction between varicella zoster virus and keratinocyte differentiation.

Varicella zoster virus (VZV) is the etiological agent of chickenpox and shingles, diseases characterized by epidermal skin blistering. Using a calcium-induced keratinocyte differentiation model we investigated the interaction between epidermal differentiation and VZV infection. RNA-seq analysis show...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Meleri Jones, Inga R Dry, Dan Frampton, Manuraj Singh, Ravinder K Kanda, Michael B Yee, Paul Kellam, Michael Hollinshead, Paul R Kinchington, Edel A O'Toole, Judith Breuer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f3b0b5731ea14552a7d5e5a614b03c70
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f3b0b5731ea14552a7d5e5a614b03c70
record_format	dspace
spelling	oai:doaj.org-article:f3b0b5731ea14552a7d5e5a614b03c702021-11-18T06:07:03ZRNA-seq analysis of host and viral gene expression highlights interaction between varicella zoster virus and keratinocyte differentiation.1553-73661553-737410.1371/journal.ppat.1003896https://doaj.org/article/f3b0b5731ea14552a7d5e5a614b03c702014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24497829/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Varicella zoster virus (VZV) is the etiological agent of chickenpox and shingles, diseases characterized by epidermal skin blistering. Using a calcium-induced keratinocyte differentiation model we investigated the interaction between epidermal differentiation and VZV infection. RNA-seq analysis showed that VZV infection has a profound effect on differentiating keratinocytes, altering the normal process of epidermal gene expression to generate a signature that resembles patterns of gene expression seen in both heritable and acquired skin-blistering disorders. Further investigation by real-time PCR, protein analysis and electron microscopy revealed that VZV specifically reduced expression of specific suprabasal cytokeratins and desmosomal proteins, leading to disruption of epidermal structure and function. These changes were accompanied by an upregulation of kallikreins and serine proteases. Taken together VZV infection promotes blistering and desquamation of the epidermis, both of which are necessary to the viral spread and pathogenesis. At the same time, analysis of the viral transcriptome provided evidence that VZV gene expression was significantly increased following calcium treatment of keratinocytes. Using reporter viruses and immunohistochemistry we confirmed that VZV gene and protein expression in skin is linked with cellular differentiation. These studies highlight the intimate host-pathogen interaction following VZV infection of skin and provide insight into the mechanisms by which VZV remodels the epidermal environment to promote its own replication and spread.Meleri JonesInga R DryDan FramptonManuraj SinghRavinder K KandaMichael B YeePaul KellamMichael HollinsheadPaul R KinchingtonEdel A O'TooleJudith BreuerPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 10, Iss 1, p e1003896 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Meleri Jones Inga R Dry Dan Frampton Manuraj Singh Ravinder K Kanda Michael B Yee Paul Kellam Michael Hollinshead Paul R Kinchington Edel A O'Toole Judith Breuer RNA-seq analysis of host and viral gene expression highlights interaction between varicella zoster virus and keratinocyte differentiation.
description	Varicella zoster virus (VZV) is the etiological agent of chickenpox and shingles, diseases characterized by epidermal skin blistering. Using a calcium-induced keratinocyte differentiation model we investigated the interaction between epidermal differentiation and VZV infection. RNA-seq analysis showed that VZV infection has a profound effect on differentiating keratinocytes, altering the normal process of epidermal gene expression to generate a signature that resembles patterns of gene expression seen in both heritable and acquired skin-blistering disorders. Further investigation by real-time PCR, protein analysis and electron microscopy revealed that VZV specifically reduced expression of specific suprabasal cytokeratins and desmosomal proteins, leading to disruption of epidermal structure and function. These changes were accompanied by an upregulation of kallikreins and serine proteases. Taken together VZV infection promotes blistering and desquamation of the epidermis, both of which are necessary to the viral spread and pathogenesis. At the same time, analysis of the viral transcriptome provided evidence that VZV gene expression was significantly increased following calcium treatment of keratinocytes. Using reporter viruses and immunohistochemistry we confirmed that VZV gene and protein expression in skin is linked with cellular differentiation. These studies highlight the intimate host-pathogen interaction following VZV infection of skin and provide insight into the mechanisms by which VZV remodels the epidermal environment to promote its own replication and spread.
format	article
author	Meleri Jones Inga R Dry Dan Frampton Manuraj Singh Ravinder K Kanda Michael B Yee Paul Kellam Michael Hollinshead Paul R Kinchington Edel A O'Toole Judith Breuer
author_facet	Meleri Jones Inga R Dry Dan Frampton Manuraj Singh Ravinder K Kanda Michael B Yee Paul Kellam Michael Hollinshead Paul R Kinchington Edel A O'Toole Judith Breuer
author_sort	Meleri Jones
title	RNA-seq analysis of host and viral gene expression highlights interaction between varicella zoster virus and keratinocyte differentiation.
title_short	RNA-seq analysis of host and viral gene expression highlights interaction between varicella zoster virus and keratinocyte differentiation.
title_full	RNA-seq analysis of host and viral gene expression highlights interaction between varicella zoster virus and keratinocyte differentiation.
title_fullStr	RNA-seq analysis of host and viral gene expression highlights interaction between varicella zoster virus and keratinocyte differentiation.
title_full_unstemmed	RNA-seq analysis of host and viral gene expression highlights interaction between varicella zoster virus and keratinocyte differentiation.
title_sort	rna-seq analysis of host and viral gene expression highlights interaction between varicella zoster virus and keratinocyte differentiation.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/f3b0b5731ea14552a7d5e5a614b03c70
work_keys_str_mv	AT melerijones rnaseqanalysisofhostandviralgeneexpressionhighlightsinteractionbetweenvaricellazostervirusandkeratinocytedifferentiation AT ingardry rnaseqanalysisofhostandviralgeneexpressionhighlightsinteractionbetweenvaricellazostervirusandkeratinocytedifferentiation AT danframpton rnaseqanalysisofhostandviralgeneexpressionhighlightsinteractionbetweenvaricellazostervirusandkeratinocytedifferentiation AT manurajsingh rnaseqanalysisofhostandviralgeneexpressionhighlightsinteractionbetweenvaricellazostervirusandkeratinocytedifferentiation AT ravinderkkanda rnaseqanalysisofhostandviralgeneexpressionhighlightsinteractionbetweenvaricellazostervirusandkeratinocytedifferentiation AT michaelbyee rnaseqanalysisofhostandviralgeneexpressionhighlightsinteractionbetweenvaricellazostervirusandkeratinocytedifferentiation AT paulkellam rnaseqanalysisofhostandviralgeneexpressionhighlightsinteractionbetweenvaricellazostervirusandkeratinocytedifferentiation AT michaelhollinshead rnaseqanalysisofhostandviralgeneexpressionhighlightsinteractionbetweenvaricellazostervirusandkeratinocytedifferentiation AT paulrkinchington rnaseqanalysisofhostandviralgeneexpressionhighlightsinteractionbetweenvaricellazostervirusandkeratinocytedifferentiation AT edelaotoole rnaseqanalysisofhostandviralgeneexpressionhighlightsinteractionbetweenvaricellazostervirusandkeratinocytedifferentiation AT judithbreuer rnaseqanalysisofhostandviralgeneexpressionhighlightsinteractionbetweenvaricellazostervirusandkeratinocytedifferentiation
_version_	1718424577725431808

RNA-seq analysis of host and viral gene expression highlights interaction between varicella zoster virus and keratinocyte differentiation.

Ejemplares similares