Oxalate-metabolising genes of the white-rot fungus Dichomitus squalens are differentially induced on wood and at high proton concentration.

Oxalic acid is a prevalent fungal metabolite with versatile roles in growth and nutrition, including degradation of plant biomass. However, the toxicity of oxalic acid makes regulation of its intra- and extracellular concentration crucial. To increase the knowledge of fungal oxalate metabolism, a tr...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Miia R Mäkelä, Outi-Maaria Sietiö, Ronald P de Vries, Sari Timonen, Kristiina Hildén
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f3b1291b27f749bda549dd18f8d9a148
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f3b1291b27f749bda549dd18f8d9a148
record_format	dspace
spelling	oai:doaj.org-article:f3b1291b27f749bda549dd18f8d9a1482021-11-18T08:33:41ZOxalate-metabolising genes of the white-rot fungus Dichomitus squalens are differentially induced on wood and at high proton concentration.1932-620310.1371/journal.pone.0087959https://doaj.org/article/f3b1291b27f749bda549dd18f8d9a1482014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24505339/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Oxalic acid is a prevalent fungal metabolite with versatile roles in growth and nutrition, including degradation of plant biomass. However, the toxicity of oxalic acid makes regulation of its intra- and extracellular concentration crucial. To increase the knowledge of fungal oxalate metabolism, a transcriptional level study on oxalate-catabolising genes was performed with an effective lignin-degrading white-rot fungus Dichomitus squalens, which has demonstrated particular abilities in production and degradation of oxalic acid. The expression of oxalic-acid decomposing oxalate decarboxylase (ODC) and formic-acid decomposing formate dehydrogenase (FDH) encoding genes was followed during the growth of D. squalens on its natural spruce wood substrate. The effect of high proton concentration on the regulation of the oxalate-catabolising genes was determined after addition of organic acid (oxalic acid) and inorganic acid (hydrochloric acid) to the liquid cultures of D. squalens. In order to evaluate the co-expression of oxalate-catabolising and manganese peroxidase (MnP) encoding genes, the expression of one MnP encoding gene, mnp1, of D. squalens was also surveyed in the solid state and liquid cultures. Sequential action of ODC and FDH encoding genes was detected in the studied cultivations. The odc1, fdh2 and fdh3 genes of D. squalens showed constitutive expression, whereas ODC2 and FHD1 most likely are the main responsible enzymes for detoxification of high concentrations of oxalic and formic acids. The results also confirmed the central role of ODC1 when D. squalens grows on coniferous wood. Phylogenetic analysis revealed that fungal ODCs have evolved from at least two gene copies whereas FDHs have a single ancestral gene. As a conclusion, the multiplicity of oxalate-catabolising genes and their differential regulation on wood and in acid-amended cultures of D. squalens point to divergent physiological roles for the corresponding enzymes.Miia R MäkeläOuti-Maaria SietiöRonald P de VriesSari TimonenKristiina HildénPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e87959 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Miia R Mäkelä Outi-Maaria Sietiö Ronald P de Vries Sari Timonen Kristiina Hildén Oxalate-metabolising genes of the white-rot fungus Dichomitus squalens are differentially induced on wood and at high proton concentration.
description	Oxalic acid is a prevalent fungal metabolite with versatile roles in growth and nutrition, including degradation of plant biomass. However, the toxicity of oxalic acid makes regulation of its intra- and extracellular concentration crucial. To increase the knowledge of fungal oxalate metabolism, a transcriptional level study on oxalate-catabolising genes was performed with an effective lignin-degrading white-rot fungus Dichomitus squalens, which has demonstrated particular abilities in production and degradation of oxalic acid. The expression of oxalic-acid decomposing oxalate decarboxylase (ODC) and formic-acid decomposing formate dehydrogenase (FDH) encoding genes was followed during the growth of D. squalens on its natural spruce wood substrate. The effect of high proton concentration on the regulation of the oxalate-catabolising genes was determined after addition of organic acid (oxalic acid) and inorganic acid (hydrochloric acid) to the liquid cultures of D. squalens. In order to evaluate the co-expression of oxalate-catabolising and manganese peroxidase (MnP) encoding genes, the expression of one MnP encoding gene, mnp1, of D. squalens was also surveyed in the solid state and liquid cultures. Sequential action of ODC and FDH encoding genes was detected in the studied cultivations. The odc1, fdh2 and fdh3 genes of D. squalens showed constitutive expression, whereas ODC2 and FHD1 most likely are the main responsible enzymes for detoxification of high concentrations of oxalic and formic acids. The results also confirmed the central role of ODC1 when D. squalens grows on coniferous wood. Phylogenetic analysis revealed that fungal ODCs have evolved from at least two gene copies whereas FDHs have a single ancestral gene. As a conclusion, the multiplicity of oxalate-catabolising genes and their differential regulation on wood and in acid-amended cultures of D. squalens point to divergent physiological roles for the corresponding enzymes.
format	article
author	Miia R Mäkelä Outi-Maaria Sietiö Ronald P de Vries Sari Timonen Kristiina Hildén
author_facet	Miia R Mäkelä Outi-Maaria Sietiö Ronald P de Vries Sari Timonen Kristiina Hildén
author_sort	Miia R Mäkelä
title	Oxalate-metabolising genes of the white-rot fungus Dichomitus squalens are differentially induced on wood and at high proton concentration.
title_short	Oxalate-metabolising genes of the white-rot fungus Dichomitus squalens are differentially induced on wood and at high proton concentration.
title_full	Oxalate-metabolising genes of the white-rot fungus Dichomitus squalens are differentially induced on wood and at high proton concentration.
title_fullStr	Oxalate-metabolising genes of the white-rot fungus Dichomitus squalens are differentially induced on wood and at high proton concentration.
title_full_unstemmed	Oxalate-metabolising genes of the white-rot fungus Dichomitus squalens are differentially induced on wood and at high proton concentration.
title_sort	oxalate-metabolising genes of the white-rot fungus dichomitus squalens are differentially induced on wood and at high proton concentration.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/f3b1291b27f749bda549dd18f8d9a148
work_keys_str_mv	AT miiarmakela oxalatemetabolisinggenesofthewhiterotfungusdichomitussqualensaredifferentiallyinducedonwoodandathighprotonconcentration AT outimaariasietio oxalatemetabolisinggenesofthewhiterotfungusdichomitussqualensaredifferentiallyinducedonwoodandathighprotonconcentration AT ronaldpdevries oxalatemetabolisinggenesofthewhiterotfungusdichomitussqualensaredifferentiallyinducedonwoodandathighprotonconcentration AT saritimonen oxalatemetabolisinggenesofthewhiterotfungusdichomitussqualensaredifferentiallyinducedonwoodandathighprotonconcentration AT kristiinahilden oxalatemetabolisinggenesofthewhiterotfungusdichomitussqualensaredifferentiallyinducedonwoodandathighprotonconcentration
_version_	1718421657898450944

Oxalate-metabolising genes of the white-rot fungus Dichomitus squalens are differentially induced on wood and at high proton concentration.

Ejemplares similares