The interferon response inhibits HIV particle production by induction of TRIM22.

Treatment of human cells with Type 1 interferons restricts HIV replication. Here we report that the tripartite motif protein TRIM22 is a key mediator. We used transcriptional profiling to identify cellular genes that were induced by interferon treatment and identified TRIM22 as one of the most stron...

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Autores principales:	Stephen D Barr, James R Smiley, Frederic D Bushman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f3b3335734414ad680f757a4f2b52cf0
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spelling	oai:doaj.org-article:f3b3335734414ad680f757a4f2b52cf02021-11-25T05:46:42ZThe interferon response inhibits HIV particle production by induction of TRIM22.1553-73661553-737410.1371/journal.ppat.1000007https://doaj.org/article/f3b3335734414ad680f757a4f2b52cf02008-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18389079/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Treatment of human cells with Type 1 interferons restricts HIV replication. Here we report that the tripartite motif protein TRIM22 is a key mediator. We used transcriptional profiling to identify cellular genes that were induced by interferon treatment and identified TRIM22 as one of the most strongly up-regulated genes. We confirmed, as in previous studies, that TRIM22 over-expression inhibited HIV replication. To assess the role of TRIM22 expressed under natural inducing conditions, we compared the effects of interferon in cells depleted for TRIM22 using RNAi and found that HIV particle release was significantly increased in the knockdown, implying that TRIM22 acts as a natural antiviral effector. Further studies showed that TRIM22 inhibited budding of virus-like particles containing Gag only, indicating that Gag was the target of TRIM22. TRIM22 did not block the release of MLV or EIAV Gag particles. Inhibition was associated with diffuse cytoplasmic staining of HIV Gag rather than accumulation at the plasma membrane, suggesting TRIM22 disrupts proper trafficking. Mutational analyses of TRIM22 showed that the catalytic amino acids Cys15 and Cys18 of the RING domain are required for TRIM22 antiviral activity. These data disclose a pathway by which Type 1 interferons obstruct HIV replication.Stephen D BarrJames R SmileyFrederic D BushmanPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 4, Iss 2, p e1000007 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Stephen D Barr James R Smiley Frederic D Bushman The interferon response inhibits HIV particle production by induction of TRIM22.
description	Treatment of human cells with Type 1 interferons restricts HIV replication. Here we report that the tripartite motif protein TRIM22 is a key mediator. We used transcriptional profiling to identify cellular genes that were induced by interferon treatment and identified TRIM22 as one of the most strongly up-regulated genes. We confirmed, as in previous studies, that TRIM22 over-expression inhibited HIV replication. To assess the role of TRIM22 expressed under natural inducing conditions, we compared the effects of interferon in cells depleted for TRIM22 using RNAi and found that HIV particle release was significantly increased in the knockdown, implying that TRIM22 acts as a natural antiviral effector. Further studies showed that TRIM22 inhibited budding of virus-like particles containing Gag only, indicating that Gag was the target of TRIM22. TRIM22 did not block the release of MLV or EIAV Gag particles. Inhibition was associated with diffuse cytoplasmic staining of HIV Gag rather than accumulation at the plasma membrane, suggesting TRIM22 disrupts proper trafficking. Mutational analyses of TRIM22 showed that the catalytic amino acids Cys15 and Cys18 of the RING domain are required for TRIM22 antiviral activity. These data disclose a pathway by which Type 1 interferons obstruct HIV replication.
format	article
author	Stephen D Barr James R Smiley Frederic D Bushman
author_facet	Stephen D Barr James R Smiley Frederic D Bushman
author_sort	Stephen D Barr
title	The interferon response inhibits HIV particle production by induction of TRIM22.
title_short	The interferon response inhibits HIV particle production by induction of TRIM22.
title_full	The interferon response inhibits HIV particle production by induction of TRIM22.
title_fullStr	The interferon response inhibits HIV particle production by induction of TRIM22.
title_full_unstemmed	The interferon response inhibits HIV particle production by induction of TRIM22.
title_sort	interferon response inhibits hiv particle production by induction of trim22.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/f3b3335734414ad680f757a4f2b52cf0
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The interferon response inhibits HIV particle production by induction of TRIM22.

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